Caspase Substrate

Ac-DEVD-pNA is a ligand for caspase-3/-7 and related cysteine proteases.

Ac-Trp-Glu-His-Asp-AMC (AC-WEHD-AMC) is a fluorescent peptide caspase-1 substrate. Ac-Trp-Glu-His-Asp-AMC is cleaved by caspase-1, enabling the measurement of caspase-1 proteolytic activity. Ac-Trp-Glu-His-Asp-AMC is cleaved by proinflammatory caspases but not by proapoptotic caspases. Ac-Trp-Glu-His-Asp-AMC requires higher enzyme concentrations to detect active caspase-1.

Ac-NKFD-AMC is a synthetic fluorescent peptide substrate for detecting non-apoptotic caspase activity. Ac-NKFD-AMC is cleaved by non-canonical activated caspases produced during monocyte-macrophage differentiation and anti-inflammatory macrophage polarization, and is not cleaved by recombinant apoptotic caspase-8 or caspase-3. Ac-NKFD-AMC can specifically distinguish non-apoptotic caspase enzymatic activity under macrophage polarization states.

Mca-VDQVDGW-Lys(Dnp)-NH2 is a fluorogenic substrate of caspase-7. Mca-VDQVDGW-K(Dnp)-NH2 can be used to quantify caspase-7 activity.

Ac-Asp-Glu-Val-Asp-pNA is a peptidic substrate of caspase-3/7.