Cat. No.: HY-114398

BAY-293

2 Cited Publications

Avis client

Based on 2 publication(s) in Google Scholar

BAY-293, a valuable chemical probe, blocks RAS activation via disruption of the KRAS-SOS1 interaction with an IC₅₀ of 21 nM. BAY-293 is a potent inhibitor of Son of Sevenless 1 (SOS1). SOS1 is the guanine nucleotide exchange factor (GEF) and activator of RAS.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

Pureté: 98.97%
CAS No.: 2244904-70-7
Formule: C₂₅H₂₈N₄O₂S
Masse moléculaire:448.58

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

BAY-293 Chemical Structure

CAS No.: 2244904-70-7

Size

Prix

Stock

Quantité

Amount: USD 0.00

* Veuillez sélectionner la quantité avant d'ajouter des articles.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (2)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target^[1]

KRAS-SOS1

21 nM (IC₅₀)

Voir tous les produits spécifiques à Isoform Ras :

Cellular Effect

Cell Line	Type	Value	Description	References
Calu-1	IC₅₀	3190 nM Compound: Chemical Probe: Compound 23, BAY-293	Antiproliferative activity against human Calu-1 cells assessed as inhibition of cell viability incubated for 72 hrs by Cell Titer Glo assay Antiproliferative activity against human Calu-1 cells assessed as inhibition of cell viability incubated for 72 hrs by Cell Titer Glo assay	[PMID: 30683722]
K562	IC₅₀	0.23 nM Compound: BAY-293	Antiproliferative activity against human K562 cells assessed as inhibition of cell proliferation Antiproliferative activity against human K562 cells assessed as inhibition of cell proliferation	[PMID: 37011767]
K562	IC₅₀	1090 nM Compound: Chemical Probe: Compound 23, BAY-293	Antiproliferative activity against human K562 cells assessed as inhibition of cell viability incubated for 72 hrs by Cell Titer Glo assay Antiproliferative activity against human K562 cells assessed as inhibition of cell viability incubated for 72 hrs by Cell Titer Glo assay	[PMID: 30683722]
MOLM-13	IC₅₀	995 nM Compound: Chemical Probe: Compound 23, BAY-293	Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell viability incubated for 72 hrs by Cell Titer Glo assay Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell viability incubated for 72 hrs by Cell Titer Glo assay	[PMID: 30683722]
NCI-H358	IC₅₀	3480 nM Compound: Chemical Probe: Compound 23, BAY-293	Antiproliferative activity against human NCI-H358 cells assessed as inhibition of cell viability incubated for 72 hrs by Cell Titer Glo assay Antiproliferative activity against human NCI-H358 cells assessed as inhibition of cell viability incubated for 72 hrs by Cell Titer Glo assay	[PMID: 30683722]

In Vitro

BAY-293 inhibits the activation of RAS in HeLa cells, with IC₅₀ values in the submicromolar range^[1].
BAY-293 (595 nM-3580 nM; 72 hours) shows efficient antiproliferative activity against wild-type KRAS cell lines (K-562, MOLM-13) and cell lines with KRAS^G12C mutation (NCI-H358, Calu-1)^[1].
BAY-293 efficiently inhibits pERK levels in K-562 cells after incubation for 60 min without affecting total protein levels of ERK^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

K-562, MOLM-13, H358 and Calu-1 cell lines
Concentration:

595-3580 nM
Incubation Time:

72 hours
Result:

IC₅₀s of 1,090±170 nM, 995±400 nM, 3,480±100 nM and 3,190±50 nM for K-562, MOLM-13, H358 and Calu-1 cells, respectively.

Chemical Information

CAS No. 2244904-70-7
Appearance Solid
Masse moléculaire 448.58
Formule C₂₅H₂₈N₄O₂S
Color White to light yellow
SMILES

COC1=C(OC)C=C(N=C(C)N=C2N[C@H](C)C3=CC(C4=C(CNC)C=CC=C4)=CS3)C2=C1
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

All

Sort by:

Journal Impact Factor

Signal Transduct Target Ther

Elaiophylin triggers paraptosis and preferentially kills ovarian cancer drug-resistant cells by inducing MAPK hyperactivation. [Abstract]

2022 Sep 12;7(1):317. PMID: 36097006
Nat Commun

Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State. [Abstract]

2026 Feb 12;17(1):1214. PMID: 41680175

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (222.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2293 mL

11.1463 mL

22.2926 mL

5 mM

0.4459 mL

2.2293 mL

4.4585 mL

10 mM

0.2229 mL

1.1146 mL

2.2293 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (276 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Hillig RC, et al. Discovery of potent SOS1 inhibitors that block RAS activation via disruption of the RAS-SOS1interaction. Proc Natl Acad Sci U S A. 2019 Feb 12;116(7):2551-2560. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2293 mL	11.1463 mL	22.2926 mL	55.7314 mL
	5 mM	0.4459 mL	2.2293 mL	4.4585 mL	11.1463 mL
	10 mM	0.2229 mL	1.1146 mL	2.2293 mL	5.5731 mL
	15 mM	0.1486 mL	0.7431 mL	1.4862 mL	3.7154 mL
	20 mM	0.1115 mL	0.5573 mL	1.1146 mL	2.7866 mL
	25 mM	0.0892 mL	0.4459 mL	0.8917 mL	2.2293 mL
	30 mM	0.0743 mL	0.3715 mL	0.7431 mL	1.8577 mL
	40 mM	0.0557 mL	0.2787 mL	0.5573 mL	1.3933 mL
	50 mM	0.0446 mL	0.2229 mL	0.4459 mL	1.1146 mL
	60 mM	0.0372 mL	0.1858 mL	0.3715 mL	0.9289 mL
	80 mM	0.0279 mL	0.1393 mL	0.2787 mL	0.6966 mL
	100 mM	0.0223 mL	0.1115 mL	0.2229 mL	0.5573 mL

Keywords

BAY-293

2 Cited Publications

100 mg Obtenir un devis

Subscribe to the

MedChemExpress

Newsletter

Nom du produit:
Cat. No.:
Quantité:
MCE Japan Authorized Agent:

Nom du produit:
CAS No.:
Species:
Pureté:
Quantité:

BAY-293

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Chemical Probe Library

Glutamine Metabolism Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Angiogenesis-Related Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Protein-protein Interaction Inhibitor Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

KRAS Targeted Compound Library

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

Anti-Cancer Compound Library

Publications Citing Use of MedChemExpress (MCE) BAY-293

Voir tous les produits spécifiques à Isoform Ras

Activité biologique

Chemical Information

Publications (2)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

BAY-293

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Chemical Probe Library

Glutamine Metabolism Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Angiogenesis-Related Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Protein-protein Interaction Inhibitor Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

KRAS Targeted Compound Library

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

Anti-Cancer Compound Library

Publications Citing Use of MedChemExpress (MCE) BAY-293

Voir tous les produits spécifiques à Isoform Ras

Activité biologique

Chemical Information

Publications (2)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

BAY-293 Related Classifications

HY-114398 Related Classifications