Bromelain (USP)

Bromelain USP completely prevents Thrombin (HY-114164)-induced human platelet aggregation and reduces Thrombin-activated human platelet adhesion to bovine aorta endothelial cells in vitro^[1].
Bromelain USP inhibits proliferation of various tumor cell lines in vitro in a concentration-dependent manner^[1].
Bromelain USP reduces the invasive potential of B16F10 mouse melanoma cells in vitro and abolishes the ability of human platelets to stimulate metastatic tumor cell invasiveness in vitro^[1].
Bromelain USP reduces CD44 expression on Molt 4/8 leukemia cells, SK-Mel 28 melanoma cells, and human peripheral blood lymphocytes in vitro, and decreases human lymphocyte adhesion to human umbilical vein endothelial cells, with fraction F9 showing the strongest CD44-modulating effect^[1].
Bromelain USP stimulates human mononuclear blood leukocytes, especially monocytes, to secrete TNF−α, Il-1β, and Il-6 in vitro^[1].
Bromelain USP is not cytotoxic to human umbilical vein endothelial cells (HUVECs)^[2].
Bromelain USP dose-dependently inhibits LPS (HY-D1056)-induced pro-inflammatory cytokine production and signaling pathway activation in human peripheral blood mononuclear cells (PBMC), THP-1 monocytic leukemia cells, human U937 macrophages, rat primary microglial cells, and mouse RAW 264.7 cells via its cysteine protease activity^[2].
Bromelain USP dose-dependently reduces expression of leukocyte surface markers involved in adhesion, migration, and activation in human peripheral blood leukocytes, mouse splenocytes, and activated mouse CD4⁺ T cells via its proteolytic activity^[2].
Bromelain USP stimulates increased production of pro-inflammatory cytokines in interferon gamma (INF-γ)-primed human peripheral blood mononuclear cells (PBMC) from healthy donors^[2].
Bromelain USP exhibits potent antifungal activity against Fusarium verticillioides, Fusarium oxysporum, and Fusarium proliferatum via its cysteine protease activity^[2].
Bromelain USP exhibits antibacterial activity against multidrug-resistant Pseudomonas aeruginosa, oral infection-associated bacteria (Enterococcus faecalis, Streptococcus mutans, Streptococcus sanguis, Staphylococcus aureus), and Alicyclobacillus species^[2].
Bromelain USP exhibits dual effects on blood coagulation, with anticoagulant and fibrinolytic activity at high concentrations, and procoagulant activity at low concentrations, and inhibits platelet aggregation in vitro^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.