cPP12-2-Lys
cPP12-2-Lys is a cyclic cell-penetrating peptide that serves as a negative control, i.e., it lacks any NatD bisubstrate moiety. cPP12-2-Lys is not a NatD inhibitor (IC50 = 9500 nM), but it possesses excellent cell-penetrating ability and can mediate the entry of conjugated bisubstrate inhibitors into non-small cell lung cancer (NSCLC) cells. cPP12-2-Lys can be used in NSCLC research.
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- Formule: C64H95N23O10S
- Masse moléculaire:1378.65
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
cPP12-2-Lys (0.15 nM-30 μM; 30 min) alone shows very weak inhibitory activity against purified NatD enzyme with an IC50 of 9500 nM[1].
cPP12-2-Lys (5 μM; 2 h) is taken up by A549 non-small cell lung cancer cells, but at lower levels than its bisubstrate inhibitor-conjugated counterpart[1].
cPP12-2-Lys (up to 10 μM; 48 h) does not reduce viability of A549 and H1299 non-small cell lung cancer cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Masse moléculaire 1378.65
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Formule C64H95N23O10S
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Sequence
Cyclo(Phe-d{Phe}-{Bta}-Arg-d{Arg}-Arg-d{Arg}-Gln)-Lys
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Sequence Shortening
Cyclo(F-d{Phe}-{Bta}-R-d{Arg}-R-d{Arg}-Q)-K
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
cPP12-2-Lys Related Classifications
HY-P11856 Related Classifications
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