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Hoechst 33342

(Synonyms: bisBenzimide H 33342; HOE 33342)
143 Cited Publications
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Based on 143 publication(s) in Google Scholar

Hoechst 33342 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.

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  • Pureté: 99.52%
  • CAS No.: 23491-52-3
  • Formule: C27H28N6O
  • Masse moléculaire:452.55
  • Stockage:

    4°C, protect from light

    * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

  • Hoechst 33342 Chemical Structure
    Hoechst 33342 Chemical Structure
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
  • Experimental Validation Results for Hoechst 33342
CAS No.: 23491-52-3
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  • Hoechst 33342 Chemical Structure
    Hoechst 33342 Chemical Structure
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    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Mar 8;15(3):200. [Abstract]

    Customer Validation
     C2C12 Cells were incubated with 1.25 μM MitoSOX in HBSS Ca/Mg for 10 min in dark at 37 °C with a 5% CO2 atmosphere. Then, cell nuclei were stained by incubating with 1 µg/mL Hoechst in HBSS, for 10 min at 37 °C in the dark, with a 5% CO2 atmosphere.

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    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Mar 17;18:91-103. [Abstract]

    Customer Validation
     The fluorescent images of HUVECs stained with NO fluorescent probe (DAF-FM DA) and Hoechst 33342 after being treated with UCATS under dark conditions or 808 nm NIR irradiation.

  • 1/16

    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Chem Eng J. 15 December 2022, 138004.

    Customer Validation
     Apoptotic cells in four groups were stained with Hoechst 33342 (for 10 min) reflected by bright blue nuclei, including hP-MSCs cultured on noncoated, L-Gel-coated (10 nM), and D-Gel coated (10 nM) plates under exposure of 500 μM H2O2 for 5 h except for the normal group, and PBS as control.

  • 1/16

    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Orthop Translat. 2022 Oct 17:38:23-31. [Abstract]

    Customer Validation
     OBs (red) and OCs were co-cultured, FAM-SGPA (green) were added to culture dish, incubated for 24 h, labelled the nucleus with Hoechst 33342 (blue, 15 min), and observed under a confocal laser scanning microscope (magnification: 630 × ).

  • 1/16

    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Orthop Translat. 2022 Oct 17:38:23-31. [Abstract]

    Customer Validation
     FAM-SGPA (green) interfered with OBs for 0.5 h, 1 h, 2 h and 4 h, labelled the nucleus with Hoechst 33342 (blue, 15 min), and observed under a confocal laser scanning microscope (magnification: 400 × ).

  • 1/16

    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2022 May 10:289:115039. [Abstract]

    Customer Validation
     MCF-7 cells were treated with QA root extract for 48 h and observed by fluorescence microscopy after Hoechst 33342 trihydrochloride (Hoechst, 1 μg/mL; 10 min) staining. Scale bar 100 μm.

  • 1/16

    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Lipid Res. 2022 May;63(5):100207. [Abstract]

    Customer Validation
     The labeling efficiency of BODIPY-loaded PLGA NPs in vitro. Fluorescence confocal images of labeled LDs in macrophages; LDs (green color, BODIPY) and nuclei (blue color, Hoechst 33342 (2 μg/mL; 2 min)); the scale bar represents 25 μm.

  • 1/16

    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Mar 4;11(10):4655-4671. [Abstract]

    Customer Validation
     Hoechst 33342 (10 µg/mL) is added to the medium of cultured megakaryocytes for 2 h at 37°C. Cells are stained with the directly coupled monoclonal antibodies PE-anti-CD41 and APC-anti-Mpl for 30 min at 4°C. Ploidy is measured in the CD41+Mpl+ cell population.

  • 1/16

    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2021 Jan 22;12(1):77. [Abstract]

    Customer Validation
     To evaluate the anti-apoptosis property of extracellular vesicles from hP-MSCs, the Hoechst 33342 (for 10 min) is used to distinguish the apoptotic cells in the three different groups.

  • 1/16

    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2021 Oct 4;220(10):e202011153. [Abstract]

    Customer Validation
     The released centromere parts are marked by arrows (blue: Hoechst; green: MajSAT-mClover; red: H2B-mCherry). Chromatin was briefly counterstained with Hoechst 33342 (5 µg/mL) before imaging. Scale bar: 5 µm (yellow) and 2 µm (white).

  • 1/16

    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2020 Sep 1;113:289-304. [Abstract]

    Customer Validation
     Hoechst 33342 staining (for 6 h) is used to distinguish apoptotic changes in H2O2-treated hP-MSCs.

  • 1/16

    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2018 Sep 12;10(36):30081-30091. [Abstract]

    Customer Validation
     Hoechst 33342 (10 min) staining shows the survival of HUVECs administrated 100 μg/mL exosomes or CS-Exo upon exposure to 400 μM H2O2.

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    Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2018;19(12):1193-1203. [Abstract]

    Customer Validation
     PC-3 stable subline cells as indicated (empty vector or CRMP4a) were seeded on cover glass in full culture media for 24 h and then stained with iFluor555-conjugated phalloidin (red) and Hoechst33342 (blue). Cells were also subjected to immunocytofluorescent staining with anti-vinculin antibodies (green) followed by visualization with AlexaFluor®488-labeld secondary antibodies. The representative microscopic images were shown from four independent experiments.

  • Hoechst 33342