Cat. No.: HY-121054

Chalcone

(Synonyms: (E/Z)-Benzylideneacetophenone)

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Based on 1 publication(s) in Google Scholar

Chalcone is isolated from Glycyrrhiza uralensis and used to synthesize chalcone derivatives. Chalcone derivatives possess varied biological and pharmacological activity, including anti-inflammatory, antioxidative, antibacterial, anticancer, and anti-parasitic activities.

For research use only. We do not sell to patients.

Purity: 99.98%
CAS No.: 94-41-7
Formula: C₁₅H₁₂O
Molecular Weight:208.26

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Chalcone Chemical Structure

CAS No.: 94-41-7

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Biological Activity
- Description
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Parasite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
2008	IC₅₀	33.4 μM Compound: 17	Cytotoxicity in human OV2008 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in human OV2008 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
A2780	IC₅₀	67.5 μM Compound: 17	Cytotoxicity in human A2780 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in human A2780 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
A2780	GI₅₀	7.3 μM Compound: Chalcone	Antiproliferative activity against human A2780 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay Antiproliferative activity against human A2780 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay	[PMID: 34262643]
A549	IC₅₀	>20 μM Compound: 1	Inhibition of TNFalpha induced NF-kappaB activation in human A549 cells by luciferase reporter gene assay Inhibition of TNFalpha induced NF-kappaB activation in human A549 cells by luciferase reporter gene assay	[PMID: 19883086]
A549	IC₅₀	69.19 μM Compound: Chalcone	Antiproliferative activity against human A549 cells assessed as cell growth inhibition incubated for 4 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition incubated for 4 hrs by MTT assay	[PMID: 32992255]
A549	IC₅₀	>10 μM Compound: 69	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by SRB assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by SRB assay	[PMID: 35026948]
A549	IC₅₀	12.2 μM Compound: 1	Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by CellTiter Aqueous One Solution MTS assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by CellTiter Aqueous One Solution MTS assay	[PMID: 37666364]
BT-20	IC₅₀	21.1 μM Compound: 17	Cytotoxicity in human BT20 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in human BT20 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
CAL-51	GI₅₀	1.85 μM Compound: SSE14101	Antiproliferative activity against human CAL51 cells assessed as growth inhibition after 3 days by SRB assay Antiproliferative activity against human CAL51 cells assessed as growth inhibition after 3 days by SRB assay	[PMID: 28743509]
CCRF-CEM	IC₅₀	12.5 μM Compound: 40	Concentration required to inhibit 50% growth of Human CEM T-lymphocytes cells Concentration required to inhibit 50% growth of Human CEM T-lymphocytes cells	[PMID: 9544201]
CHO	IC₅₀	23 μM Compound: 17	Cytotoxicity in CHO cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in CHO cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
DLD-1	EC₅₀	25 μM Compound: 4	Cytotoxicity against human DLD-1 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay Cytotoxicity against human DLD-1 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay	[PMID: 36356534]
DU-145	IC₅₀	50.1 μM Compound: 17	Cytotoxicity in human DU145 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in human DU145 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
Epithelial cell	IC₅₀	>50 μM Compound: 1	Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay Cytotoxicity against human kidney epithelial cells after 44 hrs by MTT assay	[PMID: 18378360]
FHC	CC₅₀	71 μM Compound: 4	Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay	[PMID: 36356534]
HCT-116	GI₅₀	15.7 μM Compound: C1	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 26994844]
HCT-116	GI₅₀	15.7 μM Compound: 18	Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 28177228]
HCT-116	GI₅₀	3.96 μM Compound: SSE14101	Antiproliferative activity against human HCT116 cells assessed as growth inhibition after 3 days by SRB assay Antiproliferative activity against human HCT116 cells assessed as growth inhibition after 3 days by SRB assay	[PMID: 28743509]
HCT-116	EC₅₀	11 μM Compound: 4	Cytotoxicity against p53-/- human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay Cytotoxicity against p53-/- human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay	[PMID: 36356534]
HCT-116	EC₅₀	9.5 μM Compound: 4	Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay	[PMID: 36356534]
HEK293	IC₅₀	>50 μM Compound: 1	Blockade of human K+ channel in HEK293 cells assessed as inhibition of outward delayed rectifying K+ current Blockade of human K+ channel in HEK293 cells assessed as inhibition of outward delayed rectifying K+ current	[PMID: 18032041]
HEK293	EC₅₀	11 μM Compound: 5d	Inhibition of Wnt3A/beta-catenin signaling in HEK293 cells after 24 hrs by firefly/renilla dual luciferase reporter gene assay Inhibition of Wnt3A/beta-catenin signaling in HEK293 cells after 24 hrs by firefly/renilla dual luciferase reporter gene assay	[PMID: 24275249]
HEK293	IC₅₀	10.7 μM Compound: 17	Cytotoxicity in HEK293 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in HEK293 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
HeLa	EC₅₀	>50000 nM Compound: 2a	Antimitotic activity in human HeLa cells assessed as cell density loss after 24 hrs by Hoechst 33342 staining Antimitotic activity in human HeLa cells assessed as cell density loss after 24 hrs by Hoechst 33342 staining	[PMID: 23524161]
HepG2	IC₅₀	>100 μM Compound: 1	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 22658085]
HK-2	IC₅₀	30.5 μM Compound: 1	Growth inhibition of HK2 cells by sulforhodamine assay Growth inhibition of HK2 cells by sulforhodamine assay	[PMID: 17383189]
HL-60	IC₅₀	55.4 μM Compound: 1	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 25091929]
HT-22	EC₅₀	>500 μM Compound: Chalcone	Anti-ferroptotic activity in erastin-induced ferroptosis in mouse HT-22 cells assessed as cell viability incubated for 24 hrs by MTT assay Anti-ferroptotic activity in erastin-induced ferroptosis in mouse HT-22 cells assessed as cell viability incubated for 24 hrs by MTT assay	[PMID: 37976709]
HT-29	IC₅₀	70 μM Compound: 1	Growth inhibition of HT29 cells by sulforhodamine assay Growth inhibition of HT29 cells by sulforhodamine assay	[PMID: 17383189]
HT-29	IC₅₀	45.9 μM Compound: Chalcone	Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition incubated for 4 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition incubated for 4 hrs by MTT assay	[PMID: 32992255]
HT-29	GI₅₀	>20 μM Compound: Chalcone	Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay	[PMID: 34262643]
HT-29	EC₅₀	14 μM Compound: 4	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay	[PMID: 36356534]
Jurkat	IC₅₀	17.1 μM Compound: 2a	Cytotoxicity against human Jurkat cells after 48 hrs by MTT assay Cytotoxicity against human Jurkat cells after 48 hrs by MTT assay	[PMID: 23524161]
K562	IC₅₀	3.8 μM Compound: 2ee	Antiproliferative activity against human K562 cells after 5 days by MTT assay Antiproliferative activity against human K562 cells after 5 days by MTT assay	[PMID: 19837593]
K562	IC₅₀	4.3 mM Compound: Chalcone	Induction of apoptosis in human K562 Induction of apoptosis in human K562	[PMID: 31336310]
KB	IC₅₀	>10 μM Compound: 69	Antiproliferative activity against human KB cells assessed as reduction in cell viability after 72 hrs by SRB assay Antiproliferative activity against human KB cells assessed as reduction in cell viability after 72 hrs by SRB assay	[PMID: 35026948]
L1210	IC₅₀	>100 μM Compound: 2a	Cytotoxicity against mouse L1210 cells after 24 hrs by MTT assay Cytotoxicity against mouse L1210 cells after 24 hrs by MTT assay	[PMID: 23524161]
L1210	IC₅₀	41.4 μM Compound: 40	Concentration required to inhibit 50% growth of L1210 leukemia cells Concentration required to inhibit 50% growth of L1210 leukemia cells	[PMID: 9544201]
L929	IC₅₀	6.92 μg/mL Compound: 52	Cytotoxicity against mouse L929 cells after 72 hrs by resazurin assay Cytotoxicity against mouse L929 cells after 72 hrs by resazurin assay	[PMID: 22360533]
L929	CC₅₀	89.3 μM Compound: 12	Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31000155]
MCF7	IC₅₀	63 μM Compound: 1	Growth inhibition of MCF7 cells by sulforhodamine assay Growth inhibition of MCF7 cells by sulforhodamine assay	[PMID: 17383189]
MCF7	GI₅₀	12.4 μM Compound: C1	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 26994844]
MCF7	GI₅₀	12.4 μM Compound: 18	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 28177228]
MCF7	IC₅₀	12.6 μM Compound: 17	Cytotoxicity in human MCF7 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in human MCF7 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
MCF7	IC₅₀	>10 μM Compound: 69	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by SRB assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by SRB assay	[PMID: 35026948]
MDA-MB-231	IC₅₀	6.7 μM Compound: 17	Cytotoxicity in human MDA-MB-231 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in human MDA-MB-231 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
MDA-MB-231	IC₅₀	42.8 μM Compound: Chalcone	Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 4 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 4 hrs by MTT assay	[PMID: 32992255]
MDA-MB-231	IC₅₀	>10 μM Compound: 69	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by SRB assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by SRB assay	[PMID: 35026948]
MSTO-211H	GI₅₀	8.2 μM Compound: Chalcone	Antiproliferative activity against human MSTO-211H cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay Antiproliferative activity against human MSTO-211H cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay	[PMID: 34262643]
NALM-6	IC₅₀	23.9 μM Compound: 1	Cytotoxicity against human NALM6 cells by MTT assay Cytotoxicity against human NALM6 cells by MTT assay	[PMID: 25091929]
Neutrophil	IC₅₀	13 μM Compound: 12	Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 5.5 mM glucose by luminol-amplified chemiluminescence method Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 5.5 mM glucose by luminol-amplified chemiluminescence method	[PMID: 33006891]
Neutrophil	IC₅₀	27 μM Compound: 12	Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 30 mM glucose by luminol-amplified chemiluminescence method Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 30 mM glucose by luminol-amplified chemiluminescence method	[PMID: 33006891]
NIH3T3	IC₅₀	>20 μM Compound: 1a	Inhibition of cobalt chloride-induced HIF-1 activation expressed in mouse NIH3T3 cells after 8 hrs by luciferase reporter gene assay Inhibition of cobalt chloride-induced HIF-1 activation expressed in mouse NIH3T3 cells after 8 hrs by luciferase reporter gene assay	[PMID: 21112783]
P388	IC₅₀	9.63 μM Compound: 40	Inhibition of proliferation of murine P388 cells Inhibition of proliferation of murine P388 cells	[PMID: 9544201]
PANC-1	IC₅₀	26 μM Compound: 17	Cytotoxicity in human PANC1 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in human PANC1 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
PBMC	IC₅₀	27 μM Compound: 2a	Cytotoxicity against human PBMC cells after 24 hrs by MTT assay Cytotoxicity against human PBMC cells after 24 hrs by MTT assay	[PMID: 23524161]
Peritoneal macrophage	EC₅₀	9.5 μM Compound: 10	Cytotoxicity against BALB/c mouse peritoneal macrophages incubated for 24 hrs by MTT assay Cytotoxicity against BALB/c mouse peritoneal macrophages incubated for 24 hrs by MTT assay	[PMID: 26055530]
RBL-1	IC₅₀	43 μM Compound: 18	Inhibition of 5-lipoxygenase in rat RBL1 cells Inhibition of 5-lipoxygenase in rat RBL1 cells	10.1007/s00044-013-0745-7
RBL-1	IC₅₀	43 μM Compound: 18	Inhibition of cyclooxygenase in rat RBL1 cells Inhibition of cyclooxygenase in rat RBL1 cells	10.1007/s00044-013-0745-7
TK-10	IC₅₀	42 μM Compound: 1	Growth inhibition of TK10 cells by sulforhodamine assay Growth inhibition of TK10 cells by sulforhodamine assay	[PMID: 17383189]
Tumor Cell line	IC₅₀	8.32 μM Compound: 40	Evaluation for cytotoxicity to human tumor cell lines. Evaluation for cytotoxicity to human tumor cell lines.	[PMID: 9544201]
WM-115	IC₅₀	43.1 μM Compound: 1	Cytotoxicity against human WM115 cells by MTT assay Cytotoxicity against human WM115 cells by MTT assay	[PMID: 25091929]
ZR-75-1	IC₅₀	13.7 μM Compound: 17	Cytotoxicity in human ZR-75-1 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in human ZR-75-1 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]

In Vitro

Chalcone derivatives are synthesized by Claisen-Shimidt base-catalyzed condensation of appropriate aromatic ketones or substituted aromatic ketones with benzaldehydes or substituted benzaldehydes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 94-41-7
Appearance Solid
Molecular Weight 208.26
Formula C₁₅H₁₂O
Color Off-white to light yellow
SMILES

O=C(/C=C/C1=CC=CC=C1)C2=CC=CC=C2
Synonyms

(E/Z)-Benzylideneacetophenone
Structure Classification
- Flavonoids
- Chalcones
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 Oct 15:489:144992. PMID: 40466530

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (480.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.8017 mL

24.0085 mL

48.0169 mL

5 mM

0.9603 mL

4.8017 mL

9.6034 mL

10 mM

0.4802 mL

2.4008 mL

4.8017 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Shailendra Mandge,et al. Synthesis and Characterization of Some Chalcone Derivatives. Trends in Applied Sciences Research Volume 2 (1): 52-56, 2007

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.8017 mL	24.0085 mL	48.0169 mL	120.0423 mL
	5 mM	0.9603 mL	4.8017 mL	9.6034 mL	24.0085 mL
	10 mM	0.4802 mL	2.4008 mL	4.8017 mL	12.0042 mL
	15 mM	0.3201 mL	1.6006 mL	3.2011 mL	8.0028 mL
	20 mM	0.2401 mL	1.2004 mL	2.4008 mL	6.0021 mL
	25 mM	0.1921 mL	0.9603 mL	1.9207 mL	4.8017 mL
	30 mM	0.1601 mL	0.8003 mL	1.6006 mL	4.0014 mL
	40 mM	0.1200 mL	0.6002 mL	1.2004 mL	3.0011 mL
	50 mM	0.0960 mL	0.4802 mL	0.9603 mL	2.4008 mL
	60 mM	0.0800 mL	0.4001 mL	0.8003 mL	2.0007 mL
	80 mM	0.0600 mL	0.3001 mL	0.6002 mL	1.5005 mL
	100 mM	0.0480 mL	0.2401 mL	0.4802 mL	1.2004 mL

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Publications Citing Use of MedChemExpress (MCE) Chalcone

All Parasite Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Chalcone Related Classifications

HY-121054 Related Classifications