trans-Chalcone

Cat. No.: HY-Y0598 Purity: 98.65%: FAQs
Data Sheet SDS COA Handling Instructions

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trans-Chalcone, isolated from Aronia melanocarpa skin, is a biphenolic core structure of flavonoids precursor. trans-Chalcone is a potent fatty acid synthase (FAS) and α-amylase inhibitor. trans-Chalcone causes cellcycle arrest and induces apoptosis in the breastcancer cell line MCF-7. trans-Chalcone has antifungal and anticancer activity.

For research use only. We do not sell to patients.

trans-Chalcone Chemical Structure

CAS No. : 614-47-1

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 44	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 44	In-stock	Estimated Time of Arrival: December 31
Solid
5 g	USD 40	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Other Forms of trans-Chalcone:

Chalcone In-stock

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Biological Activity
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Description

In Vitro

trans-Chalcone competitively inhibits porcine pancreatic α-amylase with a K_i of 48 μM^[2].
trans-Chalcone (30.23-98.03 μM; 24 hours) induces cell cycle arrest and apoptosis in MCF-7 cells^[1].
trans-Chalcone (20-80 μM; 24, 48 hours) reduces the expression of the apoptosis-related protein Bcl-2^[1].
trans-Chalcone (58.25 μM; 6, 24 hours) has greater inhibition of Bcl-2, induction of APAF1 and BAX, and strong induction of CIDEA in 24 hours^[1].
trans-Chalcone (24 hours) inhibits MCF-7 cell viability (IC₂₀=30.23 μM; IC₅₀=58.25 μM; IC₈₀=98.03 μM). trans-Chalcone (48 h) has IC₅₀s of 41.53 μM and 48.41 μM for MCF-7 and 3T3 cell lines, respectively. trans-Chalcone exhibits a pronounced cytotoxicity activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:	MCF-7 cell
Concentration:	30.23, 58.25, 98.03 μM
Incubation Time:	24 hours
Result:	Induced apoptosis of the breast cancer cell line.

Cell Cycle Analysis^[1]

Cell Line:	MCF-7 cell
Concentration:	30.23, 58.25, 98.03 μM
Incubation Time:	24 hours
Result:	Caused cell cycle arrest in G1.

Western Blot Analysis^[1]

Cell Line:	MCF-7 cell
Concentration:	20, 40, 80 μM
Incubation Time:	24, 48 hours
Result:	Reduced the expression of the apoptosis-related protein Bcl-2 and induced the expression of the CIDEA gene. There was marked degradation of cyclin D1 at 48 h.

RT-PCR^[1]

Cell Line:	MCF-7 cell
Concentration:	58.25 μM
Incubation Time:	6, 24 hours
Result:	Had greater inhibition of Bcl-2, induction of APAF1 and BAX, and strong induction of CIDEA in 24 hours.

Molecular Weight

208.26

Formula

C₁₅H₁₂O

CAS No.

614-47-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=CC=CC=C1)/C=C/C2=CC=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (480.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8017 mL	24.0085 mL	48.0169 mL
5 mM	0.9603 mL	4.8017 mL	9.6034 mL
10 mM	0.4802 mL	2.4008 mL	4.8017 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.65%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (248 KB) HNMR (120 KB) RP-HPLC (252 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.8017 mL	24.0085 mL	48.0169 mL	120.0423 mL
	5 mM	0.9603 mL	4.8017 mL	9.6034 mL	24.0085 mL
	10 mM	0.4802 mL	2.4008 mL	4.8017 mL	12.0042 mL
	15 mM	0.3201 mL	1.6006 mL	3.2011 mL	8.0028 mL
	20 mM	0.2401 mL	1.2004 mL	2.4008 mL	6.0021 mL
	25 mM	0.1921 mL	0.9603 mL	1.9207 mL	4.8017 mL
	30 mM	0.1601 mL	0.8003 mL	1.6006 mL	4.0014 mL
	40 mM	0.1200 mL	0.6002 mL	1.2004 mL	3.0011 mL
	50 mM	0.0960 mL	0.4802 mL	0.9603 mL	2.4008 mL
	60 mM	0.0800 mL	0.4001 mL	0.8003 mL	2.0007 mL
	80 mM	0.0600 mL	0.3001 mL	0.6002 mL	1.5005 mL
	100 mM	0.0480 mL	0.2401 mL	0.4802 mL	1.2004 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

trans-Chalcone614-47-1Inhibitorinhibitorinhibit

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