1. Immunology/Inflammation
    Stem Cell/Wnt
    JAK/STAT Signaling
  2. IFNAR
    STAT
  3. Cirsilineol

Cirsilineol 

Cat. No.: HY-119347
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Cirsilineol, a natural flavone compound, selectively inhibits IFN-γ/STAT1/T-bet signaling in intestinal CD4+ T cells. Cirsilineol has potent immunosuppressive and anti-tumor properties. Cirsilineol significantly ameliorates trinitro-benzene sulfonic acid (TNBS)-induced T-cell-mediated experimental colitis in mice.

For research use only. We do not sell to patients.

Cirsilineol Chemical Structure

Cirsilineol Chemical Structure

CAS No. : 41365-32-6

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Description

Cirsilineol, a natural flavone compound, selectively inhibits IFN-γ/STAT1/T-bet signaling in intestinal CD4+ T cells. Cirsilineol has potent immunosuppressive and anti-tumor properties. Cirsilineol significantly ameliorates trinitro-benzene sulfonic acid (TNBS)-induced T-cell-mediated experimental colitis in mice[1].

In Vitro

Cirsilineol (0.1-10 μM; 96 hours) inhibits single mixed lymphocyte reaction and Concanava A-induced T-cell proliferation in a dose-dependent manner. Cirsilineol (10 μM) does not affect T lymphocyte's viability. The inhibition of cirsilineol (10 μM) on T-cell proliferation is not due to a cytotoxic activity[1].
Cirsilineol (1-10 μM; pretreatment for 3 hours) completely inhibits IFN-γ-induced Tyr701 phosphorylation of STAT1 and JAK2 activation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: Splenocytes
Concentration: 0.1, 1, 10 μM
Incubation Time: 96 hours
Result: Inhibited single mixed lymphocyte reaction and Concanavalin A (Con A; 5 μg/mL; for 72 h)-induced T-cell proliferation in a dose-dependent manner.
Suppressed OVA323-339-specific CD4+ T-cell proliferation.

Western Blot Analysis[1]

Cell Line: Splenic CD4+ T cells
Concentration: 1, 5, 10 μM
Incubation Time: Pretreatment for 3 hours
Result: Completely inhibited IFN-γ-induced (25 ng; 30 mins) Tyr701 phosphorylation of STAT1 and JAK2 activation.
Suppressed IFN-γ-induced (25 ng; 12 hours) Th1-specific transcription factor T-bet.
In Vivo

cirsilineol (3, 10, and 30 mg/kg) significantly ameliorates TNBS-induced Th1-mediated colitis through inhibiting IFN-γ/STAT1/T-bet signaling in CD4+ T cells.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 8-10-week-old female C57BL/6, BALB/c and DO11.10 transgenic mice with TNBS (10 mg; 100 μL)[1]
Dosage: 3, 10, 30 mg/kg
Administration: IP; daily; for 11 days
Result: Showed a significant improved effect on the body weights and survival rate of mice.
Markedly reduced inflammatory infiltration, restoration of the destructive mucosal architecture and remission of edema.
Molecular Weight

344.32

Formula

C₁₈H₁₆O₇

CAS No.
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Cirsilineol
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