cPP12-2-Lys 

cPP12-2-Lys is a cyclic cell-penetrating peptide that serves as a negative control, i.e., it lacks any NatD bisubstrate moiety. cPP12-2-Lys is not a NatD inhibitor (IC₅₀ = 9500 nM), but it possesses excellent cell-penetrating ability and can mediate the entry of conjugated bisubstrate inhibitors into non-small cell lung cancer (NSCLC) cells. cPP12-2-Lys can be used in NSCLC research^[1].

cPP12-2-Lys (0.15 nM-30 μM; 30 min) alone shows very weak inhibitory activity against purified NatD enzyme with an IC₅₀ of 9500 nM^[1].
cPP12-2-Lys (5 μM; 2 h) is taken up by A549 non-small cell lung cancer cells, but at lower levels than its bisubstrate inhibitor-conjugated counterpart^[1].
cPP12-2-Lys (up to 10 μM; 48 h) does not reduce viability of A549 and H1299 non-small cell lung cancer cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1378.65

C₆₄H₉₅N₂₃O₁₀S

Cyclo(Phe-d{Phe}-{Bta}-Arg-d{Arg}-Arg-d{Arg}-Gln)-Lys

Cyclo(F-d{Phe}-{Bta}-R-d{Arg}-R-d{Arg}-Q)-K

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ho YH, et al.. A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase Activity. ACS chemical biology. 2025 Oct 17;20(10):2512-2520.  [Content Brief]