Cyclosporin C
Based on 1 Customer Validation
Cyclosporin C is a fungal metabolite that has been found in T. inflatum and has diverse biological activities, including antifungal, antiviral, and immunosuppressant properties. Cyclosporin C shows no activity against bacteria or yeasts.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 59787-61-0
- Formula: C62H111N11O13
- Molecular Weight:1218.61
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Cyclosporin C is active against isolates of Fusarium fumigatum, Aspergillus Niger, Cyclospora, Mucor and Penicillium (MICs = 0.1-5 μg/mL)[1].
Cyclosporin C (15 μg/mL) inhibits vaccinia virus replication in infected BSC40 cells by 98.82%[2].
Cyclosporin C (100 ng/mL) inhibits lymphocyte proliferation induced by the mitogens concanavalin A (HY-P2149), phytohemagglutinin L (HY-N7038), and pokeweed mitogen (HY-NP187)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CBA (H-2k), BALB/c (H-2d), C57BL/6 (H-2d) and (CBA x BALB/c), (C57BL/6 x DBA/2) male mice (6-8 weeks, 5 x 106 parental spleen cells were injected into the footpads)[3]
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Dosage:100 ng/mL
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Administration:once
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Result:Efficient in inhibiting local GVHR.
Chemical Information
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CAS No. 59787-61-0
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Appearance Solid
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Molecular Weight 1218.61
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Formula C62H111N11O13
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Color White to off-white
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SMILES
O[C@@H]([C@](C(N[C@@](C(N(CC(N([C@H](C(N[C@H](C(N([C@H]1CC(C)C)C)=O)C(C)C)=O)CC(C)C)C)=O)C)=O)([H])[C@H](O)C)=O)([H])N(C([C@@H](N(C([C@@H](N(C([C@@H](N(C([C@H](NC([C@@H](NC1=O)C)=O)C)=O)C)CC(C)C)=O)C)CC(C)C)=O)C)C(C)C)=O)C)[C@H](C)C/C=C/C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (82.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.05 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Handling Instructions (2659 KB)
References
[1]. 1. Moussaïf M, et al. Cyclosporin C is the main antifungal compound produced by Acremonium luzulae. Appl Environ Microbiol. 1997 May;63(5):1739-43. [Content Brief]
[2]. 2. Damaso CR, et al. Inhibition of vaccinia virus replication by cyclosporin A analogues correlates with their affinity for cellular cyclophilins. J Gen Virol. 1998 Feb;79 ( Pt 2):339-46. [Content Brief]
[3]. 3. Sadeg N, et al. In vitro and in vivo comparative studies on immunosuppressive properties of cyclosporines A, C, D and metabolites M1, M17 and M21. Immunopharmacol Immunotoxicol. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.8206 mL | 4.1030 mL | 8.2061 mL | 20.5152 mL |
| 5 mM | 0.1641 mL | 0.8206 mL | 1.6412 mL | 4.1030 mL | |
| 10 mM | 0.0821 mL | 0.4103 mL | 0.8206 mL | 2.0515 mL | |
| 15 mM | 0.0547 mL | 0.2735 mL | 0.5471 mL | 1.3677 mL | |
| 20 mM | 0.0410 mL | 0.2052 mL | 0.4103 mL | 1.0258 mL | |
| 25 mM | 0.0328 mL | 0.1641 mL | 0.3282 mL | 0.8206 mL | |
| 30 mM | 0.0274 mL | 0.1368 mL | 0.2735 mL | 0.6838 mL | |
| 40 mM | 0.0205 mL | 0.1026 mL | 0.2052 mL | 0.5129 mL | |
| 50 mM | 0.0164 mL | 0.0821 mL | 0.1641 mL | 0.4103 mL | |
| 60 mM | 0.0137 mL | 0.0684 mL | 0.1368 mL | 0.3419 mL | |
| 80 mM | 0.0103 mL | 0.0513 mL | 0.1026 mL | 0.2564 mL |
Cyclosporin C Related Classifications
HY-N6027 Related Classifications
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