DDO-6513
Based on 1 Customer Validation
DDO-6513 is a STING molecular glue inhibitor with an IC50 of 1.93 μM. DDO-6513 selectively targets human STING but not murine STING, and exhibits a Kd value of 151 nM for human STING. DDO-6513 induces aberrant head-to-head STING oligomerization and disrupts the formation of normal signal-competent linear STING polymers. DDO-6513 inhibits STING-dependent downstream activation of TBK1 and IRF3, and suppresses the expression of proinflammatory cytokines. DDO-6513 can be used for research on STING-related autoinflammatory diseases.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.89%
- No. CAS: 2809367-71-1
- Fòrmula: C20H18ClNO4
- Peso molecular:371.81
-
Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
-
Help & FAQs
Actividad biológica
Descripciòn
IC50 & Target
[1]|
IL-6 |
In Vitro
DDO‑6513 (3.13‑50 μM; 12‑24 h) does not exhibit significant cytotoxicity in THP‑1 and WI‑38 cells[1].
DDO‑6513 (5-20 μM; 24 h) blocks the recruitment of TBK1 to STING in cGAMP-stimulated THP-1 and HFF cells, reduces the phosphorylation levels of TBK1 and IRF3, and inhibits IRF3 dimerization and nuclear translocation[1].
DDO‑6513 induces the formation of high-order abnormal oligomers in purified human STING-CTD protein in vitro[1].
DDO‑6513 (20 μM; 12-24 h) induces the formation of STING punctate aggregates and blocks the trafficking of STING from the endoplasmic reticulum to the Golgi apparatus in HeLa and WI‑38 cells overexpressing GFP‑STING[1].
DDO‑6513 (10 μM; 24 h) reduces the elevated expression levels of ISG-related genes in PBMCs derived from AGS, DM, and SLE donors[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:THP-1 and HFF cells
-
Concentration:5, 10, 15, 20 μM
-
Incubation Time:24 h
-
Result:Blocked the recruitment of TBK1 to STING in cGAMP-stimulated THP-1 and HFF cells, reduces the phosphorylation levels of TBK1 and IRF3, and inhibits IRF3 dimerization and nuclear translocation.
-
Cell Line:HeLa and WI‑38 cells
-
Concentration:20 μM
-
Incubation Time:12 h (Hela)
24 h (WI-38) -
Result:Induced the formation of STING punctate aggregates and blocks the trafficking of STING from the endoplasmic reticulum to the Golgi apparatus in HeLa and WI‑38 cells overexpressing GFP‑STING.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:C57BL/6J STING-Hu humanized STING transgenic mice (8-12 weeks, 15 mg/kg SR-717 induced)[1]
-
Dosage:50 mg/kg
-
Administration:i.p.; once daily; 3 consecutive days (Pharmacodynamic assessment)
i.p.; once daily; 14 days (Toxicological evaluation) -
Result:Attenuated SR-717-induced IFN-β secretion and mitigated multiple systemic inflammation indicators in treated mice.
Showed no detectable abnormal histological changes in heart, liver, spleen, lung, and kidney tissues after 14 days of daily 50 mg/kg intraperitoneal administration compared to control tissue.
Chemical Information
-
No. CAS 2809367-71-1
-
Appearance Solid
-
Peso molecular 371.81
-
Fòrmula C20H18ClNO4
-
Color White to off-white
-
SMILES
O=C(C1=C(NC(C2=CC=C(C(C)(C)C)C=C2)=O)C3=CC(Cl)=CC=C3O1)O
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (268.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (13.45 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
-
Ficha de datos (298 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6895 mL | 13.4477 mL | 26.8955 mL | 67.2386 mL |
| 5 mM | 0.5379 mL | 2.6895 mL | 5.3791 mL | 13.4477 mL | |
| 10 mM | 0.2690 mL | 1.3448 mL | 2.6895 mL | 6.7239 mL | |
| 15 mM | 0.1793 mL | 0.8965 mL | 1.7930 mL | 4.4826 mL | |
| 20 mM | 0.1345 mL | 0.6724 mL | 1.3448 mL | 3.3619 mL | |
| 25 mM | 0.1076 mL | 0.5379 mL | 1.0758 mL | 2.6895 mL | |
| 30 mM | 0.0897 mL | 0.4483 mL | 0.8965 mL | 2.2413 mL | |
| 40 mM | 0.0672 mL | 0.3362 mL | 0.6724 mL | 1.6810 mL | |
| 50 mM | 0.0538 mL | 0.2690 mL | 0.5379 mL | 1.3448 mL | |
| 60 mM | 0.0448 mL | 0.2241 mL | 0.4483 mL | 1.1206 mL | |
| 80 mM | 0.0336 mL | 0.1681 mL | 0.3362 mL | 0.8405 mL | |
| 100 mM | 0.0269 mL | 0.1345 mL | 0.2690 mL | 0.6724 mL |
DDO-6513 Related Classifications
HY-W1150738 Related Classifications
Powered by Bioz