Cat. No.: HY-P1439A

RS 09 TFA

19 Cited Publications

Customer Review

Based on 19 publication(s) in Google Scholar

RS 09 TFA is an LPS (HY-D1056) peptide mimic and TLR4 agonist. RS 09 TFA can bind to TLR-4 and activate NF-κB. RS 09 TFA can function as an adjuvant in vivo, enhancing the antigen-specific immune response.

For research use only. We do not sell to patients.

Purity: 99.87%
Formula: C₃₃H₅₀F₃N₉O₁₁
Molecular Weight:805.80

Storage:

Sealed storage, away from moisture.

Powder -80°C, 2 years , -20°C, 1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

RS 09 TFA Chemical Structure

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* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (19)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

TLR4

All Toll-like Receptor (TLR) Isoforms :

All NF-κB Isoforms :

In Vitro

RS 09 (1-10 μg/mL; 24 h) TFA can stimulate TLR-4 and activate NF-κB in HEK-BLUETM-4 cells^[1].
RS 09 (5 μg/mL; 15 min-24 h) TFA can bind to TLR-4 and induce nuclear translocation of NF-κB as well as the secretion of inflammatory cytokines in RAW264.7 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

RAW264.7
Concentration:

5 μg/mL
Incubation Time:

15, 30, 60 and 120 min
Result:

Increased nuclear NF-kB levels.

In Vivo

RS 09 TFA is used as an adjuvant to co-immunize BALB/c mice with the prostate-cancer-specific antigen X-15 (conjugated to KLH). The results showed that RS 09 TFA can significantly increase the concentration of X-15-specific antibodies in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Appearance Solid
Molecular Weight 805.80
Formula C₃₃H₅₀F₃N₉O₁₁
Color White to off-white
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (19)

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Journal Impact Factor

Bioact Mater

Macrophage membrane-coated nanovesicles for dual-targeted drug delivery to inhibit tumor and induce macrophage polarization. [Abstract]

2022 Nov 9:23:69-79. PMID: 36406251
Hepatology

GPC3-based circular RNA vaccine suppresses hepatocellular carcinoma progression by activating adaptive immune responses. [Abstract]

2025 Nov 7. PMID: 41201153
J Adv Res

2'-Fucosyllactose ameliorates aging-related osteoporosis by restoring gut microbial and innate immune homeostasis. [Abstract]

2024 Nov 14:S2090-1232(24)00536-8. PMID: 39550028
Phytomedicine

Phillygenin improves diabetic nephropathy by inhibiting inflammation and apoptosis via regulating TLR4/MyD88/NF-κB and PI3K/AKT/GSK3β signaling pathways. [Abstract]

2024 Dec 3:136:156314. PMID: 39647467
Phytomedicine

A discovery of clinically approved Panlongqi Tablet for repositioning to treat osteoarthritis by inhibiting PI3K/AKT activation. [Abstract]

2022 Oct:105:154360. PMID: 35964457
NPJ Parkinsons Dis

GDNF signaling modulation by Akkermansia muciniphila ameliorates constipation-depression comorbidity in Parkinson's disease. [Abstract]

2025 Nov 28;11(1):342. PMID: 41315295
Phytother Res

Punicalagin Promotes Peripheral Nerve Repair by Enhancing Mitophagy via the TLR4/MAPK Signaling Pathway and Facilitating Angiogenesis. [Abstract]

2026 May;40(5):2632-2650. PMID: 41732027
J Ethnopharmacol

Shuxuetong injection promotes skin flap survival by inhibiting pyroptosis mediated by the TLR4/NF-κB/NLRP3 signaling pathway. [Abstract]

2025 Oct 15;355(Pt B):120749. PMID: 41106569
Clin Exp Hypertens

Cinnamaldehyde alleviates pulmonary hypertension by affecting vascular remodeling through the TLR4/NF-kB/HIF-1a pathway. [Abstract]

2025 Dec;47(1):2486829. PMID: 40171680
Ind Crops Prod

2026 Jun 18;249:123720.
Int Immunopharmacol

Eleutheroside B alleviates LPS-induced depressive-like behaviors by suppressing the TLR4/MyD88/NF-κB signaling pathway in the dentate gyrus. [Abstract]

2026 Jul 1:180:116727. PMID: 42044574
Int Immunopharmacol

Recombinant fibroblast growth factor 4 ameliorates axonal regeneration and functional recovery in acute spinal cord injury through altering microglia/macrophage phenotype. [Abstract]

2024 May 9:134:112188. PMID: 38728880
Neurochem Int

Temporal and spatial expression of Phosphodiesterase-4B after sciatic nerve compression in rats and its mechanism of action on sciatic nerve repair. [Abstract]

2025 Feb 4:105940. PMID: 39914666
Mol Neurobiol

Glycyrrhizin Attenuates White Matter Injury by Inhibiting Neuroinflammation through the HMGB1/TLR4 Pathway. [Abstract]

2025 May;62(5):6070-6087. PMID: 39707121
Biochim Biophys Acta Mol Basis Dis

Pinocembrin reduces pyroptosis to improve flap survival by modulating the TLR4/NF-κB/NLRP3 signaling pathway. [Abstract]

2025 Feb 8;1871(3):167710. PMID: 39923325
Cell Signal

ANXA2 knockdown inhibits oxidative stress and pyroptosis to alleviate epilepsy by suppressing the toll-like receptor (TLR) pathway. [Abstract]

2025 Sep 18:136:112140. PMID: 40975503
ACS Chem Neurosci

Corilagin Attenuates Neuronal Apoptosis and Ferroptosis of Parkinson's Disease through Regulating the TLR4/Src/NOX2 Signaling Pathway. [Abstract]

2025 Mar 5;16(5):968-980. PMID: 39950827
Placenta

Regulation of macrophage polarization by metformin through inhibition of TLR4/NF-κB pathway to improve pre-eclampsia. [Abstract]

2025 Jan 3:160:89-99. PMID: 39787954
Mol Cell Biol

Qi Lian Jie Ning Ameliorates DSS-Induced Colitis in Rats by Inhibition of JAK2/STAT3 and TLR4/NF-kB Pathways. [Abstract]

2025 Nov 25:1-12. PMID: 41289504

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (124.10 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 50 mg/mL (62.05 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2410 mL

6.2050 mL

12.4100 mL

5 mM

0.2482 mL

1.2410 mL

2.4820 mL

10 mM

0.1241 mL

0.6205 mL

1.2410 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (3.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Arulkumaran Shanmugam,et al. Synthetic Toll Like receptor-4 (TLR-4) Agonist Peptides as a Novel Class of Adjuvants. PLoS One. 2012;7(2):e30839. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	1.2410 mL	6.2050 mL	12.4100 mL	31.0251 mL
	5 mM	0.2482 mL	1.2410 mL	2.4820 mL	6.2050 mL
	10 mM	0.1241 mL	0.6205 mL	1.2410 mL	3.1025 mL
	15 mM	0.0827 mL	0.4137 mL	0.8273 mL	2.0683 mL
	20 mM	0.0621 mL	0.3103 mL	0.6205 mL	1.5513 mL
	25 mM	0.0496 mL	0.2482 mL	0.4964 mL	1.2410 mL
	30 mM	0.0414 mL	0.2068 mL	0.4137 mL	1.0342 mL
	40 mM	0.0310 mL	0.1551 mL	0.3103 mL	0.7756 mL
	50 mM	0.0248 mL	0.1241 mL	0.2482 mL	0.6205 mL
	60 mM	0.0207 mL	0.1034 mL	0.2068 mL	0.5171 mL
DMSO	80 mM	0.0155 mL	0.0776 mL	0.1551 mL	0.3878 mL
DMSO	100 mM	0.0124 mL	0.0621 mL	0.1241 mL	0.3103 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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RS 09 TFA

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Small Molecule Immuno-Oncology Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Reprogramming Compound Library

Oxygen Sensing Compound Library

Pyroptosis Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Transcription Factor-Targeted Library

Peptide Library

Pain-Related Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Autoimmune Disease Compound Library

Highly Selective Activators Library

Immunopotentiator Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Peptide Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Pattern Recognition Receptors Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) RS 09 TFA

All Toll-like Receptor (TLR) Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (19)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

RS 09 TFA Related Classifications

HY-P1439A Related Classifications