Cat. No.: HY-15122

Sinomenine

19 Cited Publications

Customer Review

Based on 19 publication(s) in Google Scholar

Sinomenine, an alkaloid extracted from Sinomenium acutum, is a blocker of the NF-κB activation. Sinomenine also is an activator of μ-opioid receptor.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 115-53-7
Formula: C₁₉H₂₃NO₄
Molecular Weight:329.39

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Sinomenine Chemical Structure

CAS No.: 115-53-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (19)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Sinomenine, an alkaloid extracted from Sinomenium acutum, is a blocker of the NF-κB activation^[1]. Sinomenine also is an activator of μ-opioid receptor^[2].

IC₅₀ & Target

μ Opioid Receptor/MOR

All NF-κB Isoforms :

All Opioid Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human A549 cells by CCK8 assay Antiproliferative activity against human A549 cells by CCK8 assay	[PMID: 37939862]
HCT-116	IC₅₀	>50 μM Compound: SIN	Cytotoxicity against human HCT-116 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Cytotoxicity against human HCT-116 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 38417218]
HepG2	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human HepG2 cells by CCK8 assay Antiproliferative activity against human HepG2 cells by CCK8 assay	[PMID: 37939862]
HT-29	IC₅₀	>50 μM Compound: SIN	Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 38417218]
K562	IC₅₀	37.24 μM Compound: 1	Antiproliferative activity against human K562 cells by CCK8 assay Antiproliferative activity against human K562 cells by CCK8 assay	[PMID: 37939862]
KB	IC₅₀	>759 μM Compound: Sinomenine	Cytotoxicity against human KB cells after 72 hrs Cytotoxicity against human KB cells after 72 hrs	[PMID: 11141105]
MCF7	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human MCF7 cells by CCK8 assay Antiproliferative activity against human MCF7 cells by CCK8 assay	[PMID: 37939862]
MDA-MB-231	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells by CCK8 assay Antiproliferative activity against human MDA-MB-231 cells by CCK8 assay	[PMID: 37939862]
NCM460	IC₅₀	>50 μM Compound: SIN	Cytotoxicity against human NCM460 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Cytotoxicity against human NCM460 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 38417218]
NIH3T3	IC₅₀	>100 μM Compound: 1	Cytotoxicity against mouse NIH/3T3 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 23357309]
PANC-1	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human PANC-1 cells by CCK8 assay Antiproliferative activity against human PANC-1 cells by CCK8 assay	[PMID: 37939862]
RAW264.7	IC₅₀	>100 μM Compound: 1	Cytotoxicity against mouse RAW264.7 cells after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells after 24 hrs by MTT assay	[PMID: 21530276]
RAW264.7	IC₅₀	>200 μM Compound: 1	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method	[PMID: 22325804]
RAW264.7	IC₅₀	>200 μM Compound: 1	Cytotoxicity against mouse RAW264.7 cells after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells after 24 hrs by MTT assay	[PMID: 22325804]

In Vitro

Cell viability gradually decreased with increasing Sinomenine concentration. The migration ability of MDA-MB-231 cells is significantly weakened by 0.25, 0.5, and 1 mM of Sinomenine treatment. The wound-healing assay reveals that 0.25 and 0.5 mM Sinomenine significantly suppress the healing of the wound. When the MDA-MB-231 cells are treated with 0.5 mM Sinomenine, the healing progress is about 50%, but in the group treated with 0.25 mM Sinomenine and the untreated control, the healing is about 80% and nearly 95%, respectively. The IB assay following inhibitor of NF-κB (IκB) antibody IP shows that the binding of NF-κB to IκB is inhibited by Sinomenine treatment in a dose-dependent manne^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Sinomenine (i.p.) produces antinociception in the hot plate and tail flick tests in male rats at 40 mg/kg, but not at lower doses (10 or 20 mg/kg). At 10 to 40 mg/kg Sinomenine does not produce any observable side effect such as sedation, allergy or motor impairments. Antinociception is also seen mice at 60 min following 80 mg/kg i.p. Sinomenine, but not at lower doses (20 or 40 mg/kg), in the tail flick test. Sinomenine at 80 mg/kg i.p. does not produce any observable side effects in mice. I.p or p.o. Sinomenine at 40 or 80 mg/kg dose-dependently reduces mechanical hypersensitivity in nerve injured mice. I.p. Sinomenine at 40 mg/kg, but not lower doses or vehicle, significantly decreases mechanical and cold allodynia for up to 240 min without producing motor deficits or sedation^[3]. At doses of 10 to 40 mg/kg, Sinomenine dose-dependently increases the paw withdrawal threshold. In non-chronic constriction injury (CCI) healthy rats, Sinomenine at the dose range of 10 to 40 mg/kg does not change the immobility behavior in the forced swimming test^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 115-53-7
Appearance Solid
Molecular Weight 329.39
Formula C₁₉H₂₃NO₄
Color White to off-white
SMILES

OC(C1=C2C[C@@]3([H])[C@](C=C4OC)([H])[C@@]1(CCN3C)CC4=O)=C(C=C2)OC
Structure Classification
- Alkaloids
- Piperidine Alkaloids
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (19)

All Cell Proliferation/Viability AssayFlow CytometryRT-PCRWBHistological Imaging/Staining

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Journal Impact Factor

Acta Pharm Sin B

Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway. [Abstract]

2021 Nov;11(11):3465-3480. PMID: 34900530
Phytomedicine

Fangchinoline suppresses nasopharyngeal carcinoma progression by inhibiting SQLE to regulate the PI3K/AKT pathway dysregulation. [Abstract]

2025 May:140:156484. PMID: 40090046
Phytother Res

Cognitive protection of sinomenine in type 2 diabetes mellitus through regulating the EGF/Nrf2/HO-1 signaling, the microbiota-gut-brain axis, and hippocampal neuron ferroptosis. [Abstract]

2023 Aug;37(8):3323-3341. PMID: 37036428
Free Radic Biol Med

Cold atmospheric plasma conveys selectivity on triple negative breast cancer cells both in vitro and in vivo. [Abstract]

2018 Aug 20:124:205-213. PMID: 29870749

Sinomenine purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2018 Aug 20:124:205-213. [Abstract]

Total and phosphorylation levels of p65 in MCF7 and MDAMB231 after Sinomenine hydrochloride (SH) treatment at 24 h.
Anal Chem

Noninvasive Dynamic Cell Viability Monitoring Based on Diffraction Height Fingerprint Spectra. [Abstract]

2025 Dec 19. PMID: 41417584
PLoS Biol

A high-throughput behavioral screening platform for measuring chemotaxis by C. elegans. [Abstract]

2024 Jun 27;22(6):e3002672. PMID: 38935621
Life Sci

Sinomenine ameliorates myocardial ischemia/reperfusion injury by inhibiting mitochondrial oxidative stress-mediated PANoptosis and ferroptosis via α7nAChR. [Abstract]

2026 Mar 1:388:124199. PMID: 41520699
Drug Des Devel Ther

Sinomenine Alleviates Rheumatoid Arthritis by Suppressing the PI3K-Akt Signaling Pathway, as Demonstrated Through Network Pharmacology, Molecular Docking, and Experimental Validation. [Abstract]

2024 Aug 6:18:3523-3545. PMID: 39135759
Eur J Pharmacol

Sinomenine alleviates ulcerative colitis by targeting FXR to regulate arachidonic acid metabolism and Th17/Treg homeostasis. [Abstract]

2026 Jun 11:1030:178976. PMID: 42269904
Eur J Pharmacol

Sinomenine modulates autoantigen-specific immune responses by inhibiting dendritic cell activation in experimental arthritis. [Abstract]

2026 May 10:1023:178881. PMID: 42000020
World J Stem Cells

Sinomenine increases osteogenesis in mice with ovariectomy-induced bone loss by modulating autophagy. [Abstract]

2024 May 26;16(5):486-498. PMID: 38817333
J Dairy Sci

Sinomenine hydrochloride ameliorates fatty acid-induced bovine mammary epithelial cells' oxidative stress and inflammation via enhancing autophagy activity. [Abstract]

2025 Nov 7:S0022-0302(25)00911-7. PMID: 41207443
J Inflamm Res

Sinomenine Ameliorates IL-1β-Induced Intervertebral Disc Degeneration in Rats Through Suppressing Inflammation and Oxidative Stress via Keap1/Nrf2/NF-κB Signaling Pathways. [Abstract]

2023 Oct 20:16:4777-4791. PMID: 37881650

Sinomenine purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2023 Oct 20:16:4777-4791. [Abstract]

Effect of Sinomenine (SN) (1, 2, 4, 8, 16 μM) on the activity of NPCs.

Sinomenine purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2023 Oct 20:16:4777-4791. [Abstract]

Pretreatment with Sinomenine (SN) (4 μM) for 8 hours decreased ROS content and apoptosis.

Sinomenine purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2023 Oct 20:16:4777-4791. [Abstract]

Pre-treatment with Sinomenine (SN) (4 μM) for 8 hours reversed IL-1β induced the expression of DCN and COL2A1 by RT-PCR.

Sinomenine purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2023 Oct 20:16:4777-4791. [Abstract]

Pre-treatment with Sinomenine (SN) (4 μM) for 8 hours reversed IL-1β induced the expression of DCN and COL2A1 by Western Blot.

Sinomenine purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2023 Oct 20:16:4777-4791. [Abstract]

HE staining showed that Sinomenine (SN) improves IDD by suppressing the inflammatory response.
Naunyn Schmiedebergs Arch Pharmacol

Sinomenine alleviates gouty inflammation by inhibiting macrophage M1 polarization and neutrophil extracellular trap formation. [Abstract]

2025 May 17. PMID: 40381009
Immun Inflamm Dis

High-dose sinomenine attenuates ischemia/reperfusion-induced hepatic inflammation and oxidative stress in rats with diabetes mellitus. [Abstract]

2024 Jun;12(6):e1271. PMID: 38888355
Chem Biol Drug Des

Sinomenine attenuates lipopolysaccharide-induced inflammation and apoptosis of WI-38 cells by reducing GSTM1 expression. [Abstract]

2023 Sep;102(3):434-443. PMID: 36303295
Exp Ther Med

Sinomenine activation of Nrf2 signaling prevents inflammation and cerebral injury in a mouse model of ischemic stroke. [Abstract]

2021 Jun;21(6):647. PMID: 33968178
Heliyon

Sinomenine ameliorates bleomycin-induced pulmonary fibrosis by inhibiting the differentiation of fibroblast into myofibroblast. [Abstract]

2024 Jun 24;10(13):e33314. PMID: 39050413
bioRxiv

An efficient behavioral screening platform classifies natural products and other chemical cues according to their chemosensory valence in C. elegans. [Abstract]

2024 Apr 3:2023.06.02.542933. PMID: 37333363

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (151.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

1M HCl : 25 mg/mL (75.90 mM; ultrasonic and adjust pH to 1 with HCl)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0359 mL

15.1796 mL

30.3591 mL

5 mM

0.6072 mL

3.0359 mL

6.0718 mL

10 mM

0.3036 mL

1.5180 mL

3.0359 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[1]

The MDA-MB-231 human triple negative and 4T1 mouse breast cancer cell lines are used in this study. For the experiments, the cells are grown in 24-well plates at 3.5×10⁴/well. Following incubation for 24 or 48 h in medium containing different concentrations of Sinomenine, proliferation of the cells are detected with Cell Counting Kit-8 solution according to the manufacturer's instructions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[4]

Male Sprague-Dawley rats weighing of 250 to 300 g are used in this experiment. For the duration of action of acute Sinomenine study, different doses of Sinomenine (10 to 40 mg/kg) are administered 1 day after surgery and then paw withdrawal threshold is measured every 30 min for 4 hours. For the study involving daily Sinomenine treatment, mechanical hyperalgesia measure is performed 3 h after daily drug treatment. For antagonist studies, antagonists were given 10 min prior to 40 mg/kg Sinomenine administration^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Song L, et al. Sinomenine inhibits breast cancer cell invasion and migration by suppressing NF-κB activation mediated by IL-4/miR-324-5p/CUEDC2 axis. Biochem Biophys Res Commun. 2015 Aug 28;464(3):705-10. [Content Brief]

[2]. Wang MH, et al. Activation of opioid mu-receptor by sinomenine in cell and mice.Neurosci Lett. 2008 Oct 10;443(3):209-12. [Content Brief]

[3]. Gao T, et al. Analgesic effect of sinomenine in rodents after inflammation and nerve injury. Eur J Pharmacol. 2013 Dec 5;721(1-3):5-11. [Content Brief]

[4]. Zhu Q, et al. Antinociceptive effects of sinomenine in a rat model of neuropathic pain. Sci Rep. 2014 Dec 1;4:7270. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
1M HCl / DMSO	1 mM	3.0359 mL	15.1796 mL	30.3591 mL	75.8979 mL
	5 mM	0.6072 mL	3.0359 mL	6.0718 mL	15.1796 mL
	10 mM	0.3036 mL	1.5180 mL	3.0359 mL	7.5898 mL
	15 mM	0.2024 mL	1.0120 mL	2.0239 mL	5.0599 mL
	20 mM	0.1518 mL	0.7590 mL	1.5180 mL	3.7949 mL
	25 mM	0.1214 mL	0.6072 mL	1.2144 mL	3.0359 mL
	30 mM	0.1012 mL	0.5060 mL	1.0120 mL	2.5299 mL
	40 mM	0.0759 mL	0.3795 mL	0.7590 mL	1.8974 mL
	50 mM	0.0607 mL	0.3036 mL	0.6072 mL	1.5180 mL
	60 mM	0.0506 mL	0.2530 mL	0.5060 mL	1.2650 mL
DMSO	80 mM	0.0379 mL	0.1897 mL	0.3795 mL	0.9487 mL
DMSO	100 mM	0.0304 mL	0.1518 mL	0.3036 mL	0.7590 mL

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Sinomenine

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Publications Citing Use of MedChemExpress (MCE) Sinomenine

All NF-κB Isoforms

All Opioid Receptor Isoforms

Biological Activity

Chemical Information

Publications (19)