Cat. No.: HY-N0592A

Demethyleneberberine chloride

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Demethyleneberberine chloride is a blood-brain barrier-permeable antioxidant. Demethyleneberberine chloride exerts antioxidant effects by targeting mitochondria, activates the AMPK signaling pathway to regulate lipid metabolism, and inhibits inflammation-related pathways such as NF-κB and MAPK. Demethyleneberberine chloride can be used in the research of inflammatory and other diseases.

For research use only. We do not sell to patients.

Purity: 98.90%
CAS No.: 16705-03-6
Formula: C₁₉H₁₈ClNO₄
Molecular Weight:359.80

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Demethyleneberberine chloride Chemical Structure

CAS No.: 16705-03-6

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All NF-κB Isoforms :

All AMPK Isoforms :

All p38 MAPK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
PC-12	CC₅₀	>150 μM Compound: 12	Cytotoxicity against rat PC12 cells assessed as reduction in cell viability incubated for 24 hrs by WST-8 assay Cytotoxicity against rat PC12 cells assessed as reduction in cell viability incubated for 24 hrs by WST-8 assay	[PMID: 33611188]

In Vitro

Demethyleneberine (50 μM; 2 h) chloride neutralizes 55% of H₂O₂-induced ROS in HepG2 cells^[1].
Demethyleneberberine (50 μM; 1 week) chloride reverses ethanol-induced mitochondrial membrane potential depolarization in ethanol-exposed HepG2 cells^[1].
Demethyleneberberine (0-50 μM; 0-24 h) chloride shows no toxicity to HepG2 cells at concentrations up to 50 μM, reduces cellular lipid accumulation by 25% at 10 μM, and activates the AMPK pathway in a dose- and time-dependent manner^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Demethyleneberberine (40 mg/kg; i.p.; 1 hour after each ethanol exposure; 3 exposures total) chloride protects liver mitochondria of ICR mice against oxidative stress damage and ameliorates their acute alcoholic liver injury^[1].
Demethyleneberberine (40 mg/kg per day; i.p.; daily; 5 weeks) chloride prevents chronic alcohol intake-induced liver injury and steatosis in ICR mice by inhibiting oxidative stress pathways and restoring fatty acid oxidation^[1].
Demethyleneberberine (20-40 mg/kg; i.p.; daily administration; for 4 consecutive weeks) chloride alleviates MCD-induced non-alcoholic fatty liver disease (NAFLD) in ICR mice and spontaneous NAFLD in db/db mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

ICR mice (male, 8 weeks old, 24-26 g, acute alcoholic liver injury model)^[1]
Dosage:

40 mg/kg
Administration:

i.p.; 1 hour after each ethanol exposure; 3 exposures total
Result:

Attenuated ethanol-induced microsteatosis, swelling, and apoptosis in liver cells.
Blunted elevated serum ALT levels.
Blocked ethanol-induced 33% decline in mitochondrial GSH and GPx activity and 250% elevation in mitochondrial TBARS formation.
Alleviated ethanol-mediated mitochondrial swelling.
Significantly recovered ethanol-reduced mitochondrial AST activity by 50%.
Ameliorated ethanol-induced mitochondrial ultrastructural damages and reduced lipid droplet accumulation.

Animal Model:

C57BLKS/J-Leprdb/Leprdb (db/db) mice (8-week-old male; spontaneous NAFLD model due to leptin deficiency)^[2]
Dosage:

20 mg/kg
Administration:

i.p.; daily; 4 weeks
Result:

Reduced liver/body index.
Decreased hepatic TG and TC levels and improved liver function (reduced serum ALT).
Decreased hepatic MDA.
Reduced lipid droplet accumulation.
Activated AMPK phosphorylation and increased phosphorylation of ACC.
Reduced expression of lipogenic genes and inflammatory genes (IL-1β, TNFα).
Increased expression of fatty acid β-oxidation genes (ACO, MCAD, CPT1A) and lipid transport genes (MTTP, ApoB).

Chemical Information

CAS No. 16705-03-6
Appearance Solid
Molecular Weight 359.80
Formula C₁₉H₁₈ClNO₄
Color Light yellow to yellow
SMILES

COC1=C(OC)C2=C[N+]3=C(C4=CC(O)=C(O)C=C4CC3)C=C2C=C1.[Cl-]
Structure Classification
- Alkaloids
- Other Alkaloids
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (3)

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Journal Impact Factor

Phytomedicine

Huaier-derived arnicolide D induces ferroptosis in gastric cancer via SRC/TNF-mediated GPX4 inactivation. [Abstract]

2026 May:154:158029. PMID: 41818944
Molecules

Discovery of Nine Dipeptidyl Peptidase-4 Inhibitors from Coptis chinensis Using Virtual Screening, Bioactivity Evaluation, and Binding Studies. [Abstract]

2024 May 14;29(10):2304. PMID: 38792165
Chem Biol Drug Des

CD163 and TYROBP Are Two Therapeutic Targets for Hyperglycemia-Induced Mesangial Cell Stress. [Abstract]

2025 Dec;106(6):e70213. PMID: 41329505

Solvent & Solubility

In Vitro:

DMSO : 1.92 mg/mL (5.34 mM; ultrasonic and warming and adjust pH to 2 with 1 M HCl and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

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Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7793 mL

13.8966 mL

27.7932 mL

5 mM

0.5559 mL

2.7793 mL

5.5586 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhang P, et al. Demethyleneberberine, a natural mitochondria-targeted antioxidant, inhibits mitochondrial dysfunction, oxidative stress, and steatosis in alcoholic liver disease mouse model. J Pharmacol Exp Ther. 2015 Jan;352(1):139-47. [Content Brief]

[2]. Qiang X, et al. Demethyleneberberine attenuates non-alcoholic fatty liver disease with activation of AMPK and inhibition of oxidative stress. Biochem Biophys Res Commun. 2016 Apr 15;472(4):603-9. [Content Brief]

[3]. Li J, et al. Pharmacokinetics, Tissue Distribution and Excretion of Demethyleneberberine, a Metabolite of Berberine, in Rats and Mice. Molecules. 2023;28(23):7725. Published 2023 Nov 23. [Content Brief]

[4]. Saklani P, et al. Demethyleneberberine, a potential therapeutic agent in neurodegenerative disorders: a proposed mechanistic insight. Mol Biol Rep. 2022;49(10):10101-10113. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7793 mL	13.8966 mL	27.7932 mL	69.4830 mL
DMSO	5 mM	0.5559 mL	2.7793 mL	5.5586 mL	13.8966 mL

Keywords

Demethyleneberberine chloride

3 Cited Publications

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Demethyleneberberine chloride

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Publications Citing Use of MedChemExpress (MCE) Demethyleneberberine chloride

All NF-κB Isoforms

All AMPK Isoforms

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Demethyleneberberine chloride Related Classifications

HY-N0592A Related Classifications