Cat. No.: HY-N0925

Tetrahydroberberine

(Synonyms: Canadine)

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Tetrahydroberberine is a different kind of living thing that can be extended and divided into parts. Tetrahydroberberine is a kind of effective D2 receptor antagonistic force. Tetrahydroberberine has the ability to strengthen the stomach and relieve the pressure on the stomach.

For research use only. We do not sell to patients.

Purity: 98.31%
CAS No.: 522-97-4
Formula: C₂₀H₂₁NO₄
Molecular Weight:339.39

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Tetrahydroberberine Chemical Structure

CAS No.: 522-97-4

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

5-HT_1A Receptor

All Dopamine Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>30 μM Compound: 9	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 20594848]
HCT-15	IC₅₀	>30 μM Compound: 9	Cytotoxicity against human HCT15 cells by SRB assay Cytotoxicity against human HCT15 cells by SRB assay	[PMID: 20594848]
PC-12	CC₅₀	>150 μM Compound: 15	Cytotoxicity against rat PC12 cells assessed as reduction in cell viability incubated for 24 hrs by WST-8 assay Cytotoxicity against rat PC12 cells assessed as reduction in cell viability incubated for 24 hrs by WST-8 assay	[PMID: 33611188]
SK-MEL-2	IC₅₀	27.94 μM Compound: 9	Cytotoxicity against human SK-MEL-2 cells by SRB assay Cytotoxicity against human SK-MEL-2 cells by SRB assay	[PMID: 20594848]
SK-OV-3	IC₅₀	>30 μM Compound: 9	Cytotoxicity against human SKOV3 cells by SRB assay Cytotoxicity against human SKOV3 cells by SRB assay	[PMID: 20594848]
THP-1	IC₅₀	8.35 nM Compound: 3	Inhibition of tissue factor procoagulant activity in LPS-stimulated human THP1 cells preincubated for 1 hr before LPS addition measured after 5 hrs Inhibition of tissue factor procoagulant activity in LPS-stimulated human THP1 cells preincubated for 1 hr before LPS addition measured after 5 hrs	[PMID: 23199480]

In Vitro

Tetrahydroberberine (100 μM) blocks K(ATP) channels in sharply isolated dopaminergic (DA) neurons in rat substantia nigra pars compacta (SNc) and restores membrane hyperpolarization induced by 1 μM rotenone (HY-B1756)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Tetrahydroberberine (30 μg/kg; IV) causes a dramatic increase in gastric regulatory capacity in beagle dogs, significantly enhances gastric contractility and shortens contraction intervals in rats, and enhances gastric motility function in the upper gastrointestinal tract^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 522-97-4
Appearance Solid
Molecular Weight 339.39
Formula C₂₀H₂₁NO₄
Color Light yellow to yellow
SMILES

COC1=CC=C(CC23)C(CN2CCC(C3=C4)=CC5=C4OCO5)=C1OC
Synonyms

Canadine
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

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Journal Impact Factor

J Agric Food Chem

Tetrahydroberberine Prevents Ovariectomy-Induced Bone Loss by Inhibiting RANKL-Induced Osteoclastogenesis and Promoting Osteoclast Apoptosis. [Abstract]

2024 Sep 18;72(37):20383-20395. PMID: 39238071
Mol Neurobiol

Activation of the Nrf2 Signaling Pathway by Tetrahydroberberine Suppresses Ferroptosis and Enhances Functional Recovery Following Spinal Cord Injury. [Abstract]

2025 Jul;62(7):8439-8456. PMID: 40011360

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (73.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9465 mL

14.7323 mL

29.4646 mL

5 mM

0.5893 mL

2.9465 mL

5.8929 mL

10 mM

0.2946 mL

1.4732 mL

2.9465 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lee TH, et al. Tetrahydroberberine, an isoquinoline alkaloid isolated from corydalis tuber, enhances gastrointestinal motor function. J Pharmacol Exp Ther. 2011 Sep;338(3):917-24. [Content Brief]

[2]. Niwa M, et al. Dopaminergic unique affinity of tetrahydroberberine and l-tetrahydroberberine-d-camphor sulfonate. Pharmacology. 1991;43(6):329-36. [Content Brief]

[3]. Wu C, et al. Tetrahydroberberine blocks ATP-sensitive potassium channels in dopamine neurons acutely-dissociated from rat substantia nigra pars compacta. Neuropharmacology. 2010 Dec;59(7-8):567-72. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9465 mL	14.7323 mL	29.4646 mL	73.6616 mL
	5 mM	0.5893 mL	2.9465 mL	5.8929 mL	14.7323 mL
	10 mM	0.2946 mL	1.4732 mL	2.9465 mL	7.3662 mL
	15 mM	0.1964 mL	0.9822 mL	1.9643 mL	4.9108 mL
	20 mM	0.1473 mL	0.7366 mL	1.4732 mL	3.6831 mL
	25 mM	0.1179 mL	0.5893 mL	1.1786 mL	2.9465 mL
	30 mM	0.0982 mL	0.4911 mL	0.9822 mL	2.4554 mL
	40 mM	0.0737 mL	0.3683 mL	0.7366 mL	1.8415 mL
	50 mM	0.0589 mL	0.2946 mL	0.5893 mL	1.4732 mL
	60 mM	0.0491 mL	0.2455 mL	0.4911 mL	1.2277 mL

Keywords

Tetrahydroberberine

2 Cited Publications

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Tetrahydroberberine

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Publications Citing Use of MedChemExpress (MCE) Tetrahydroberberine

All Dopamine Receptor Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Tetrahydroberberine Related Classifications

HY-N0925 Related Classifications