Dihydrolipoic Acid
Based on 2 publication(s) in Google Scholar
Dihydrolipoic Acid (DHLA) is an excellent antioxidant capable of scavenging almost any oxygen-centered radical. Dihydrolipoic acid exhibits anti-inflammatory properties in various diseases. Dihydrolipoic Acid exerts a preventive effect via ERK/Nrf2/HO-1/ROS/NLRP3 pathway in LPS-induced sickness behavior rats. Dihydrolipoic Acid can be used for the reaserch of depression.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 462-20-4
- Formula: C8H16O2S2
- Molecular Weight:208.34
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Storage:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Dihydrolipoic Acid
More
Biological Activity
Dihydrolipoic Acid is an antioxidant. DHLA is capable of scavenging ?OH radicals and scavenging superoxide radical anions with a rate constant of 3.3×105 M-1 s-1. Dihydrolipoic Acid is an excellent antioxidant, and that is very important because O2?- is a relatively mild oxidant that is therefore much more selective and can be useful to discriminate between the antioxidant capacities of different substrates[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult male Sprague-Dawley (SD) rats (weight, 200-220 g)[2]
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Dosage:15 mg/kg, 30 mg/kg, 60 mg/kg
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Administration:Injected intraperitoneally daily
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Result:Treatment with 30 mg/kg and 60 mg/kg improved the body weight gain as compared to the LPS group.
Chemical Information
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CAS No. 462-20-4
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Appearance Liquid (Density: 1.132 g/cm3)
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Molecular Weight 208.34
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Formula C8H16O2S2
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Color Colorless to light yellow
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SMILES
OC(CCCCC(CCS)S)=O
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Synonyms
DHLA
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Free Radic Biol Med
Dihydrolipoic acid suppresses ferroptosis in chondrocytes to ameliorate the progression of osteoarthritis by modulating the FOXO1/TXNIP signaling pathway. [Abstract]2026 Mar 16:246:169-180. PMID: 41539373 -
Eur J Pharmacol
Exploring the effect of chlorogenic acid on oxidative stress and autophagy in dry eye mice via the AMPK/ULK1 pathway. [Abstract]2025 Mar 15:991:177311. PMID: 39892448
Solvent & Solubility
DMSO : 100 mg/mL (479.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (3.98 mM); Clear solution
This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.83 mg/mL (3.98 mM); Clear solution
This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (758 KB)
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- Italian - IT (758 KB)
- Korean - KR (758 KB)
- Portuguese - PT (758 KB)
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Handling Instructions (2659 KB)
References
[1]. Romina Castañeda-Arriaga, et al. Lipoic acid and dihydrolipoic acid. A comprehensive theoretical study of their antioxidant activity supported by available experimental kinetic data. J Chem Inf Model. 2014 Jun 23;54(6):1642-52. [Content Brief]
[2]. Hetao Bian, et al. Dihydrolipoic acid protects against lipopolysaccharide-induced behavioral deficits and neuroinflammation via regulation of Nrf2/HO-1/NLRP3 signaling in rat. J Neuroinflammation. 2020 May 25;17(1):166. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.7998 mL | 23.9992 mL | 47.9985 mL | 119.9962 mL |
| 5 mM | 0.9600 mL | 4.7998 mL | 9.5997 mL | 23.9992 mL | |
| 10 mM | 0.4800 mL | 2.3999 mL | 4.7998 mL | 11.9996 mL | |
| 15 mM | 0.3200 mL | 1.5999 mL | 3.1999 mL | 7.9997 mL | |
| 20 mM | 0.2400 mL | 1.2000 mL | 2.3999 mL | 5.9998 mL | |
| 25 mM | 0.1920 mL | 0.9600 mL | 1.9199 mL | 4.7998 mL | |
| 30 mM | 0.1600 mL | 0.8000 mL | 1.5999 mL | 3.9999 mL | |
| 40 mM | 0.1200 mL | 0.6000 mL | 1.2000 mL | 2.9999 mL | |
| 50 mM | 0.0960 mL | 0.4800 mL | 0.9600 mL | 2.3999 mL | |
| 60 mM | 0.0800 mL | 0.4000 mL | 0.8000 mL | 1.9999 mL | |
| 80 mM | 0.0600 mL | 0.3000 mL | 0.6000 mL | 1.5000 mL | |
| 100 mM | 0.0480 mL | 0.2400 mL | 0.4800 mL | 1.2000 mL |
Dihydrolipoic Acid Related Classifications
HY-116807 Related Classifications
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