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Cat. No.: HY-130723

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AMPK activator 2

AMPK activator 2 (compound 7a), a fluorine-containing proguanil derivative, up-regulates AMPK signal pathway and downregulates mTOR/4EBP1/p70S6K. AMPK activator 2 inhibits proliferation and migration of human cancer cell lines (UMUC3, T24, A549).

For research use only. We do not sell to patients.

CAS No.: 2410961-69-0
Formula: C₁₃H₁₈F₃N₅
Molecular Weight:301.31

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (264 KB)

AMPK activator 2 Chemical Structure

CAS No.: 2410961-69-0

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
Chemical Information
Protocols (2)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

AMPK

All AMPK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	2.1 μM Compound: 7a	Antiproliferative activity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31864776]
A549	IC₅₀	1.3 μM Compound: 7a	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31864776]
HCT-116	IC₅₀	3.5 μM Compound: 7a	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31864776]
HUVEC	IC₅₀	8.5 μM Compound: 7a	Cytotoxicity against human HUVEC cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human HUVEC cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31864776]
T-24	IC₅₀	3.1 μM Compound: 7a	Antiproliferative activity against human T24 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human T24 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31864776]
UMUC3	IC₅₀	2 μM Compound: 7a	Antiproliferative activity against human UM-UC-3 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human UM-UC-3 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31864776]

Chemical Information

CAS No. 2410961-69-0
Molecular Weight 301.31
Formula C₁₃H₁₈F₃N₅
SMILES

N=C(NC(NC1=CC=C(C(F)(F)F)C=C1)=N)NCC(C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

Cell migration
Cell migration is a method that plays an important role in wound healing, cell differentiation, embryonic development, etc.
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (264 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Di Xiao, et al. Synthesis, biological evaluation and anti-proliferative mechanism of fluorine-containing proguanil derivatives. Bioorg Med Chem. 2020 Jan 15;28(2):115258. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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100 mg

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