L-Cysteic acid
Based on 1 Customer Validation
L-Cysteic acid is a non-proteinogenic amino acid. L-Cysteic acid modification enhances the inhibitory effects on ROS and MDA. The combination of L-Cysteic acid with Demecolcine (HY-N0282) maximizes CSADCase immunoreactivity. L-Cysteic acid modification enhances the antioxidant activity of collagen peptide CP. L-Cysteic acid can be used in studies of oxidative stress-induced damage.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 498-40-8
- Formula: C3H7NO5S
- Molecular Weight:169.16
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Storage:
RT, protect from light.
In solvent -80°C, 1 year , -20°C, 6 months
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
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Human Endogenous Metabolite |
In Vitro
The L-Cysteic acid-modified peptide SCP (0.2-1.0 mg/mL) exhibits potent hydroxyl radical scavenging activity in a cell-free system, significantly outperforming GSH and reference peptides[1].
L-Cysteic acid modification of the peptide (25-100 μM; 24 h) promotes the proliferation of Chang liver cells, particularly at concentrations of 50 and 100 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Chang liver cells
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Concentration:25-100 μM
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Incubation Time:24 h
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Result:Increased the cell vitality at concentrations of 50 and 100 μM compared to the control.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Albino rabbits (young adult, 1.5-2 kg)[4]
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Dosage:2 ng/kg
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Administration:i.v.; 1-2 hr before fixation
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Result:Increased specific CSADCase immunoreactivity above that in normal untreated animals.
Enhanced CSADCase immunoreactivity intensity without changing the number of reactive cells.
Revealed sagittal microbands with seven reactive bands in the anterior lobe vermis (one midline band of 12-16 Purkinje cells across and three pairs of lateral bands of 18-24 cells).
Showed at least two bands in the hemispheres of lobules VI and VII varying from 8 to 18 Purkinje cells.
Showed at least 60-65% of Purkinje cells were CSADCase positive in the paraflocculus and flocculus.
Chemical Information
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CAS No. 498-40-8
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Appearance Solid
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Molecular Weight 169.16
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Formula C3H7NO5S
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Color White to off-white
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SMILES
N[C@H](C(O)=O)CS(=O)(O)=O
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, protect from light
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (147.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.78 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.78 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
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Data Sheet (311 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 5.9116 mL | 29.5578 mL | 59.1156 mL | 147.7891 mL |
| 5 mM | 1.1823 mL | 5.9116 mL | 11.8231 mL | 29.5578 mL | |
| 10 mM | 0.5912 mL | 2.9558 mL | 5.9116 mL | 14.7789 mL | |
| 15 mM | 0.3941 mL | 1.9705 mL | 3.9410 mL | 9.8526 mL | |
| 20 mM | 0.2956 mL | 1.4779 mL | 2.9558 mL | 7.3895 mL | |
| 25 mM | 0.2365 mL | 1.1823 mL | 2.3646 mL | 5.9116 mL | |
| 30 mM | 0.1971 mL | 0.9853 mL | 1.9705 mL | 4.9263 mL | |
| 40 mM | 0.1478 mL | 0.7389 mL | 1.4779 mL | 3.6947 mL | |
| 50 mM | 0.1182 mL | 0.5912 mL | 1.1823 mL | 2.9558 mL | |
| 60 mM | 0.0985 mL | 0.4926 mL | 0.9853 mL | 2.4632 mL | |
| 80 mM | 0.0739 mL | 0.3695 mL | 0.7389 mL | 1.8474 mL | |
| 100 mM | 0.0591 mL | 0.2956 mL | 0.5912 mL | 1.4779 mL |
L-Cysteic acid Related Classifications
HY-124009 Related Classifications
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