DMPO
Based on 8 publication(s) in Google Scholar
DMPO is a cell permeable hydrophillic spin trap agent for superoxide detection.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 3317-61-1
- Formula: C6H11NO
- Molecular Weight:113.16
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) DMPO
More- Redox Biol. 2025 Feb 21:81:103561. [Abstract]
- J Colloid Interface Sci. 2025 Apr 15:693:137611. [Abstract]
- Adv Healthc Mater. 2025 Oct 15:e03594. [Abstract]
- Sep Purif Technol. 2025 Dec 31.
- Phytomedicine. 2025 Oct 20:148:157423. [Abstract]
- ACS Appl Mater Interfaces. 2025 Mar 12;17(10):15333-15346. [Abstract]
- Int J Food Microbiol. 2025 May 21:440:111276. [Abstract]
- Chem Bio Eng. 2025 Dec 18.
Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
Biological Activity
DMPO (100 μM, 24 h) attenuates SIN-1 (3-Morpholinosydnonimine (HY-126849)) (500 μM, 2 h)-mediated cytotoxicity and ROS generation in BAEC or HEK293 cells[3].
DMPO (100 μM, 24 h) increases NO levels via increasing eNOS activity and phospho-eNOS levels in BAEC cells[3].
DMPO (1-100 mM) inhibits FMLP and concanavalin A (HY-P2149) induced ·O2- secretion in neutrophils[4].
DMPO (50 mM, 24 h) inhibits Lipopolysaccharides (HY-D1056)-triggered M1-linked pro-inflammatory cytokine (IL-1β, IL-6 and TNF-α) and NO production in RAW 264,6 cells[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BAEC cells
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Concentration:100 μM
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Incubation Time:12 and 24 h
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Result:Increased p-eNOS and p-Akt level.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3317-61-1
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Appearance Solid-Liquid Mixture
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Molecular Weight 113.16
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Formula C6H11NO
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Color Colorless to off-white
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SMILES
CC1(C)[N+]([O-])=CCC1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (8)
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Journal Impact Factor
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Most Recent
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Redox Biol
2025 Feb 21:81:103561. PMID: 40020452 -
J Colloid Interface Sci
Redox-driven hybrid nanoenzyme dynamically activating ferroptosis and disulfidptosis for hepatocellular carcinoma theranostics. [Abstract]2025 Apr 15:693:137611. PMID: 40253866 -
Adv Healthc Mater
Microenvironment-Responsive Cu-MOF Nanoplatform Activates Disulfiram for Synergistic Bacterial Killing and Enhanced Infected Wound Healing. [Abstract]2025 Oct 15:e03594. PMID: 41097868 -
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Phytomedicine
Arnicolide C induces ROS-mediated modulation of PI3K/Akt and MAPK pathways to suppress MYC in hepatocellular carcinoma. [Abstract]2025 Oct 20:148:157423. PMID: 41138574 -
ACS Appl Mater Interfaces
Engineering Hierarchically Grooved Structures for Enhancing Interfacial Solar Thermal Water Purification. [Abstract]2025 Mar 12;17(10):15333-15346. PMID: 39999438 -
Int J Food Microbiol
Inactivation mechanisms of carvacrol on Salmonella Typhimurium and its combined inhibitory effects with blue light-405 nm for chicken meat preservation. [Abstract]2025 May 21:440:111276. PMID: 40409141 -
DMPO purchased from MedChemExpress. Usage Cited in: Chem Bio Eng. 2025 Dec 18.
DMPO (5 μL, 10 M). ESR spectra of ·OH for ZnO2 and SNP/ZnO2 at various pH conditions.
DMPO purchased from MedChemExpress. Usage Cited in: Chem Bio Eng. 2025 Dec 18.
DMPO (5 μL, 10 M). ESR spectra of ·O2 for ZnO2 and SNP/ZnO2 at various pH conditions.
Solvent & Solubility
H2O : 125 mg/mL (1104.63 mM; Need ultrasonic)
DMSO : 100 mg/mL (883.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (22.09 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (22.09 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Ranguelova K, Mason RP. The fidelity of spin trapping with DMPO in biological systems. Magn Reson Chem. 2011;49(4):152-158. [Content Brief]
[2]. Konaka R, Kawai M, Noda H, Kohno M, Niwa R. Synthesis and evaluation of DMPO-type spin traps. Free Radic Res. 1995;23(1):15-25. [Content Brief]
[3]. Das A, et al. Reversal of SIN-1-induced eNOS dysfunction by the spin trap, DMPO, in bovine aortic endothelial cells via eNOS phosphorylation. Br J Pharmacol. 2014 May;171(9):2321-34. [Content Brief]
[4]. Britigan BE, Hamill DR. Effect of the spin trap 5,5 dimethyl-1-pyrroline-N-oxide (DMPO) on human neutrophil function: novel inhibition of neutrophil stimulus-response coupling? Free Radic Biol Med. 1990;8(5):459-70. [Content Brief]
[5]. Zhai Z, et al. The spin trap 5,5-dimethyl-1-pyrroline N-oxide inhibits lipopolysaccharide-induced inflammatory response in RAW 264.7 cells. Life Sci. 2012 Mar 10;90(11-12):432-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 8.8370 mL | 44.1852 mL | 88.3704 mL | 220.9261 mL |
| 5 mM | 1.7674 mL | 8.8370 mL | 17.6741 mL | 44.1852 mL | |
| 10 mM | 0.8837 mL | 4.4185 mL | 8.8370 mL | 22.0926 mL | |
| 15 mM | 0.5891 mL | 2.9457 mL | 5.8914 mL | 14.7284 mL | |
| 20 mM | 0.4419 mL | 2.2093 mL | 4.4185 mL | 11.0463 mL | |
| 25 mM | 0.3535 mL | 1.7674 mL | 3.5348 mL | 8.8370 mL | |
| 30 mM | 0.2946 mL | 1.4728 mL | 2.9457 mL | 7.3642 mL | |
| 40 mM | 0.2209 mL | 1.1046 mL | 2.2093 mL | 5.5232 mL | |
| 50 mM | 0.1767 mL | 0.8837 mL | 1.7674 mL | 4.4185 mL | |
| 60 mM | 0.1473 mL | 0.7364 mL | 1.4728 mL | 3.6821 mL | |
| 80 mM | 0.1105 mL | 0.5523 mL | 1.1046 mL | 2.7616 mL | |
| 100 mM | 0.0884 mL | 0.4419 mL | 0.8837 mL | 2.2093 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
DMPO Related Classifications
HY-107690 Related Classifications
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