Estradiol 3-glucuronide
Based on 1 publication(s) in Google Scholar
Estradiol 3-glucuronide is an estrogen metabolite, which is a glucuronide conjugate formed from Estradiol (HY-B0141) via catalysis by uridine diphosphate glucuronosyltransferases in tissues such as the liver. Estradiol 3-glucuronide is a potent substrate of Mrp2, with an S50 value of 55.7 μM. Estradiol 3-glucuronide achieves hepatobiliary transport in hepatocytes through basolateral uptake via OATP1B1, OATP1B3 and OATP2B1, as well as apical efflux via MRP2 and BCRP.
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- Pureté : 97.62%
- CAS No.: 15270-30-1
- Formule: C24H32O8
- Masse moléculaire:448.51
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Stockage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Estradiol 3-glucuronide
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Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| Sf9 | IC50 |
120 μM
Compound: Estradiol 3-glucuronide
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TP_TRANSPORTER: inhibition of E217betaG uptake in membrane vesicles from MRP4-expressing Sf9 cells
TP_TRANSPORTER: inhibition of E217betaG uptake in membrane vesicles from MRP4-expressing Sf9 cells
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[PMID: 12523936] |
| Sf9 | IC50 |
80 μM
Compound: Estradiol 3-glucuronide
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TP_TRANSPORTER: inhibition of DHEAS in membrane vesicles from MRP4-expressing Sf9 cells
TP_TRANSPORTER: inhibition of DHEAS in membrane vesicles from MRP4-expressing Sf9 cells
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[PMID: 12523936] |
In Vitro
Estradiol 3-glucuronide (1-5 μM; 2 minutes) is a substrate for OATP1B1, OATP1B3, and OATP2B1 in transfected HEK293T cells, with OATP2B1 exhibiting the highest transport efficiency (Vmax/Km = 33.2) and acting as the primary transporter at low concentrations[2].
Estradiol 3-glucuronide (5 μM; 2 minutes) uptake by OATP1B1, OATP1B3, and OATP2B1 in transfected HEK293T cells is differentially inhibited by known OATP substrates/inhibitors, with estrone-3-sulfate showing the strongest inhibitory effect on OATP1B1-mediated uptake (IC50 = 0.05 μM)[2].
Estradiol 3-glucuronide (50 μM; 2 minutes) weakly to moderately inhibits OATP1B3-, OATP2B1-mediated estrone-3-sulfate uptake and OATP1B1-, OATP1B3-, OATP2B1-mediated Rosuvastatin (HY-17504A) uptake in transfected HEK293T cells[2].
Estradiol 3-glucuronide (30-110 nM; up to 20 min) is a substrate of rat Mrp2 expressed in Sf9 cell membrane vesicles, with an S50 of 55.7 μM and slight negative cooperativity in transport[1].
Estradiol 3-glucuronide (0.4-400 μM) competitively and completely inhibits rat Mrp2-mediated transport of Estradiol 17-glucuronide in Sf9 cell membrane vesicles with an IC50 of 14.2 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 15270-30-1
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Appearance Solid
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Masse moléculaire 448.51
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Formule C24H32O8
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Color White to off-white
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SMILES
C[C@@]12[C@](CC[C@@H]2O)([H])[C@@]3([H])[C@@](CC1)([H])C4=CC=C(O[C@@H]5O[C@@H]([C@H]([C@@H]([C@H]5O)O)O)C(O)=O)C=C4CC3
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvant et solubilité
In Vitro:
H2O : 1 mg/mL (2.23 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Research Protocol for Endocrine Diseases
Endocrine diseases often arise from disrupted hormone production, hormone signaling, or target-tissue responsiveness; for diabetes-focused endocrine disease models, insulin signaling regulates glucose uptake, hepatic glucose output, lipid metabolism, and β-cell compensation. Type 2 diabetes develops through interacting defects in insulin resistance, β-cell dysfunction, adipose inflammation, hepatic glucose overproduction, altered incretin signaling, and ectopic lipid metabolism. A major unresolved question is whether endocrine dysfunction is driven primarily by target-tissue insulin resistance, intrinsic β-cell failure, immune/inflammatory stress, or combined multi-organ failure that differs by disease stage.
Pureté et documentation
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Fiche technique (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Gerk PM, et al. Estradiol 3-glucuronide is transported by the multidrug resistance-associated protein 2 but does not activate the allosteric site bound by estradiol 17-glucuronide. Drug Metab Dispos. 2004;32(10):1139-1145. [Content Brief]
[2].
Li L, et al. Hepatic uptake of estradiol-3-glucuronide and estradiol-3-sulfate-17β-glucuronide by human organic anion transporting polypeptides 1B1, 1B3, and 2B1. Drug Metab Dispos. 2025 Sep;53(9):100132.
[Content Brief]
[3].
Nambara T, et al. A direct radioimmunoassay of estradiol 3-glucuronide using specific antiserum. Chem Pharm Bull (Tokyo). 1983 Oct;31(10):3583-8.
[Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.2296 mL | 11.1480 mL | 22.2960 mL | 55.7401 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Estradiol 3-glucuronide Related Classifications
HY-W585842 Related Classifications
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