2. Immunology/Inflammation
  3. Interleukin Related
  4. F8-IL-4

F8-IL-4 is an immune cytokine targeting IL-4. F8-IL-4 specifically delivers IL-4 to inflammatory sites via binding to targets expressed on neovascular vessels. F8-IL-4 alleviates collagen-induced arthritis in mice by regulating T cell subsets and macrophage polarization. When combined with Dexamethasone (HY-14648), F8-IL-4 produces a synergistic and long-lasting therapeutic effect, and prevents arthritis recurrence after drug withdrawal by maintaining anti-inflammatory cell phenotypes and cytokine profiles. F8-IL-4 can be used in the research of collagen-induced arthritis.

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F8-IL-4

F8-IL-4 Chemical Structure

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F8-IL-4 Related Antibodies

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Description

F8-IL-4 is an immune cytokine targeting IL-4. F8-IL-4 specifically delivers IL-4 to inflammatory sites via binding to targets expressed on neovascular vessels. F8-IL-4 alleviates collagen-induced arthritis in mice by regulating T cell subsets and macrophage polarization. When combined with Dexamethasone (HY-14648), F8-IL-4 produces a synergistic and long-lasting therapeutic effect, and prevents arthritis recurrence after drug withdrawal by maintaining anti-inflammatory cell phenotypes and cytokine profiles. F8-IL-4 can be used in the research of collagen-induced arthritis[1].

Species Reactivity

Human
IC50 & Target

IL-4

 

In Vivo

F8-IL-4 (100 μg per mouse; intraperitoneally; on day 1, 4, and 7 post-disease onset; 10 days) alleviates collagen-induced arthritis in DBA/1 mice by regulating T cell subsets and macrophage polarization; when combined with Dexamethasone (HY-14648), it produces a synergistic and long-lasting therapeutic effect, and prevents arthritis recurrence after drug withdrawal by maintaining anti-inflammatory cell phenotypes and cytokine profiles[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: DBA/1 (10- to 12-week-old male; collagen-induced arthritis model)[1]
Dosage: 100 μg per mouse
Administration: intraperitoneally; on day 1, 4, and 7 post-disease onset; 10 days
Result: Reduced arthritis scores, increased IL-4 levels in joints and serum, decreased IL-1β, IL-17A, IL-6, and GM-CSF levels in the claws, and increased TNF-α levels in the claws.
Significantly increased the total number and percentage of IL-4-secreting T_H2 cells in the joints and significantly decreased the number of IL-17A-secreting CD4+ and TCRγδ T cells.
Increased the percentage of CD206+ macrophages in the claws and decreased the percentage of macrophages expressing MHC class II molecules in the claws.
Decreased the expression of Il12b (p40) mRNA in the spleen.
Produced a synergistic therapeutic effect when used in combination with dexamethasone.
Significantly increased serum IL-10 levels when used in combination with dexamethasone.
When used in combination with dexamethasone:
Significantly decreased the levels of IL-1β, IL-6, GM-CSF, and IL-12p70 in the claws.
Significantly increased the proportion of CD25+FoxP3+ Treg cells among CD4+ T cells in the joints and significantly decreased the T_H17/Treg cell ratio.
Significantly increased the percentage of CD206+ macrophages in the claws and significantly decreased the percentage of total macrophages and macrophages highly expressing MHC class II molecules in the claws.
Significantly increased the expression of arginase 1, IL-10, and Chil3 (Ym1) mRNA in the spleen and significantly decreased the expression of Nos2 and Il12b (p40) mRNA.
Significantly decreased the total number of cells, T_H17 cells, IL-17A+ TCRγδ T cells, and neutrophils in the claws.
Significantly decreased the levels of IL-17A, IL-1β, and IL-12 in the claws and significantly increased the serum IL-10 level.
Significantly increased the percentage of CD206+ macrophages in the claws and decreased the percentage of MHC class II positive macrophages.
Significantly increased the expression of arginase 1 and IL-10 mRNA in the spleen, and decreased the expression of IL-12b (p40) mRNA.
Gene ID

2335  [NCBI]

Accession
Target

Fibronectin
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
SMILES

[F8-IL-4]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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F8-IL-4 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

F8-IL-4Interleukin RelatedILimmune cytokinecollagen-induced arthritisDBA/1 miceInhibitorinhibitorinhibit

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