CDCA-Met
Based on 1 Customer Validation
CDCA-Met is a conjugate of CDCA (HY-76847) and Met. CDCA-Met can be produced by bacteria. CDCA-Met is an inducer that elevates IFNγ levels, with limited effect on interleukin-17. CDCA-Met can be used in the study of inflammatory bowel disease.
For research use only. We do not sell to patients.
- Purity : 99.92%
- CAS No.: 3067552-33-1
- Formula: C29H49NO5S
- Molecular Weight:523.77
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Description
IC50 & Target
[2]|
Microbial Metabolite |
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3067552-33-1
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Appearance Solid
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Molecular Weight 523.77
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Formula C29H49NO5S
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Color White to off-white
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SMILES
O[C@H]1[C@]2([H])[C@@](CC[C@]3([C@@]2([H])CC[C@]3([H])[C@H](C)CCC(N[C@H](C(O)=O)CCSC)=O)C)([H])[C@@]4([C@](C[C@@H](CC4)O)([H])C1)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (190.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.77 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.77 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.9092 mL | 9.5462 mL | 19.0923 mL | 47.7309 mL |
| 5 mM | 0.3818 mL | 1.9092 mL | 3.8185 mL | 9.5462 mL | |
| 10 mM | 0.1909 mL | 0.9546 mL | 1.9092 mL | 4.7731 mL | |
| 15 mM | 0.1273 mL | 0.6364 mL | 1.2728 mL | 3.1821 mL | |
| 20 mM | 0.0955 mL | 0.4773 mL | 0.9546 mL | 2.3865 mL | |
| 25 mM | 0.0764 mL | 0.3818 mL | 0.7637 mL | 1.9092 mL | |
| 30 mM | 0.0636 mL | 0.3182 mL | 0.6364 mL | 1.5910 mL | |
| 40 mM | 0.0477 mL | 0.2387 mL | 0.4773 mL | 1.1933 mL | |
| 50 mM | 0.0382 mL | 0.1909 mL | 0.3818 mL | 0.9546 mL | |
| 60 mM | 0.0318 mL | 0.1591 mL | 0.3182 mL | 0.7955 mL | |
| 80 mM | 0.0239 mL | 0.1193 mL | 0.2387 mL | 0.5966 mL | |
| 100 mM | 0.0191 mL | 0.0955 mL | 0.1909 mL | 0.4773 mL |
CDCA-Met Related Classifications
HY-188213 Related Classifications
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Keywords
- CDCA-Met
- 3067552-33-1
- Drug Derivative
- Amino Acid Derivatives
- Endogenous Metabolite
- IFNAR
- FXR binding
- IFN-γ
- RORγt+ CD4+ T cells
- deoxycholic acid conjugate isomer
- human inflammatory bowel disease cohort
- inflammatory bowel disease
- interferon-gamma
- interleukin seventeen
- microbiota-produced methionine-conjugated chenodeoxycholic acid bile acid amidate
- mouse BMDC-naive CD4+ T cell co-cultures
- Inhibitor
- inhibitor
- inhibit