3-Hydroxyindole
Based on 1 Customer Validation
3-Hydroxyindole is a hydroxyindole compound. 3-Hydroxyindole can be isolated from the fruits of Aristotelia chilensis. 3-Hydroxyindole inhibits Ferroptosis and scavenges DPPH free radicals. 3-Hydroxyindole protects against β-amyloid-induced toxicity. 3-Hydroxyindole exhibits antioxidant activity. 3-Hydroxyindole can be used in research related to neurodegenerative diseases.
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- Pureza : 87.60%
- No. CAS: 480-93-3
- Fòrmula: C8H7NO
- Peso molecular:133.15
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Almacenamiento:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HT-22 | EC50 |
7.9 μM
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Anti-ferroptotic activity against mouse hippocampal HT-22 cells induced by erastin, assessed as cell viability via calcein AM assay after 24 h co-treatment.
Anti-ferroptotic activity against mouse hippocampal HT-22 cells induced by erastin, assessed as cell viability via calcein AM assay after 24 h co-treatment.
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40670345 |
| HT-22 | EC50 |
3.5 μM
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Anti-ferroptotic activity against mouse hippocampal HT-22 cells induced by RSL3, assessed as cell viability via calcein AM assay after 24 h co-treatment.
Anti-ferroptotic activity against mouse hippocampal HT-22 cells induced by RSL3, assessed as cell viability via calcein AM assay after 24 h co-treatment.
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40670345 |
| HT-22 | EC50 |
1.6 μM
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Anti-ferroptotic activity against mouse hippocampal HT-22 cells induced by FINO2, assessed as cell viability via calcein AM assay after 24 h co-treatment.
Anti-ferroptotic activity against mouse hippocampal HT-22 cells induced by FINO2, assessed as cell viability via calcein AM assay after 24 h co-treatment.
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40670345 |
| N27 | EC50 |
1.5 μM
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Anti-ferroptotic activity against rat dopaminergic N27 cells induced by RSL3, assessed as cell viability via calcein AM assay after 24 h co-treatment.
Anti-ferroptotic activity against rat dopaminergic N27 cells induced by RSL3, assessed as cell viability via calcein AM assay after 24 h co-treatment.
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40670345 |
| N27 | EC50 |
2.2 μM
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Anti-ferroptotic activity against rat dopaminergic N27 cells induced by FINO2, assessed as cell viability via calcein AM assay after 24 h co-treatment.
Anti-ferroptotic activity against rat dopaminergic N27 cells induced by FINO2, assessed as cell viability via calcein AM assay after 24 h co-treatment.
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40670345 |
In Vitro
3-Hydroxyindole potently scavenges DPPH radicals in a cell-free system with an IC50 of 0.87 ppm, showing greater activity than α-tocopherol[1].
3-Hydroxyindole potently scavenges crocin radicals in a cell-free system with an IC50 of 0.67 ppm[1].
3-Hydroxyindole potently inhibits lipid peroxidation in brain homogenate ex vivo with an IC50 of 1.29 ppm[1].
3-Hydroxyindole (20 μM; 22-24 h) potently inhibits ferroptosis induced by Erastin (HY-15763),RSL3 (HY-100218A), and FINO2 (HY-129457) in HT-22 mouse hippocampal neurons, with EC50 values of 7.9 μM, 3.5 μM, and 1.6 μM respectively[2].
3-Hydroxyindole (25 μM; 24 h) does not protect HT-22 mouse hippocampal neurons from non-ferroptotic toxicity induced by Rotenone (HY-B1756), indicating its protective effects are specific to ferroptosis[2].
3-Hydroxyindole (24 h) potently inhibits ferroptosis induced by RSL3 and FINO2 in N27 rat dopaminergic neurons, with EC50 values of 1.5 μM and 2.2 μM respectively[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT-22 mouse hippocampal neurons
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Concentration:20 μM (LDH assay; lipid peroxidation assay); dose-dependent concentrations (calcein AM viability assays)
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Incubation Time:22-24 h (LDH assay; lipid peroxidation assay); 24 h (calcein AM viability assays)
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Result:Significantly suppressed erastin-induced LDH release and lipid peroxidation at 20 μM.
Protected HT-22 cells from erastin-induced viability loss at 20 μM.
Had EC50 values of 7.9 μM against erastin, 3.5 μM against RSL3, and 1.6 μM against FINO2, representing the highest anti-ferroptotic potency among tested hydroxyindoles.
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Cell Line:HT-22 mouse hippocampal neurons
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Concentration:25 μM
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Incubation Time:24 h
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Result:Maintained cell viability when co-treated with erastin and Liproxstatin-1.
Did not preserve erastin-induced glutathione depletion.
Did not increase baseline glutathione levels.\nFailed to protect HT-22 cells from rotenone-induced viability loss.
Failed to prevent rotenone-induced ATP depletion.
Chemical Information
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No. CAS 480-93-3
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Appearance Solid
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Peso molecular 133.15
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Fòrmula C8H7NO
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Color Green to dark green
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SMILES
C1=CC=C2C(=C1)C(=CN2)O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvente y solubilidad
In Vitro:
DMSO : 2 mg/mL (15.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Céspedes CL, et al. Antioxidant activity of an unusual 3-hydroxyindole derivative isolated from fruits of Aristotelia chilensis (Molina) Stuntz. Zeitschrift fur Naturforschung. C, Journal of biosciences. 2009;64(9-10):759-62. [Content Brief]
[2]. Jakaria M, et al. The role of hydroxyindoles in protecting neuronal cultures from ferroptosis. Cell death discovery. 2025 Jul 16;11(1):329. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 7.5103 mL | 37.5516 mL | 75.1033 mL | 187.7582 mL |
| 5 mM | 1.5021 mL | 7.5103 mL | 15.0207 mL | 37.5516 mL | |
| 10 mM | 0.7510 mL | 3.7552 mL | 7.5103 mL | 18.7758 mL | |
| 15 mM | 0.5007 mL | 2.5034 mL | 5.0069 mL | 12.5172 mL |
3-Hydroxyindole Related Classifications
HY-W277951 Related Classifications
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Keywords
- 3-Hydroxyindole
- 480-93-3
- Ferroptosis
- Free Radical Scavengers
- Amyloid-β
- PC12 rat pheochromocytoma cells
- neurodegenerative diseases
- radical scavenger
- blood-brain barrier
- ferroptosis inhibitor
- Aristotelia chilensis
- antioxidant
- HT-22 mouse hippocampal neurons
- lipid peroxidation inhibitor
- N27 rat dopaminergic neurons
- Inhibitor
- inhibitor
- inhibit