AMARA peptide
AMARA peptide is a synthetic peptide substrate of sucrose non-fermenting-1-related protein kinase 1 (SnRK1). AMARA peptide contains residues required for SnRK1 phosphorylation, retains the minimal motif recognized by SnRK1, and when this substrate is recognized by cauliflower SnRK1 HRK-A, the hydrophobic residues at the relative positions P-5 and P+4 of the phosphorylated serine require a critical spacing. AMARA peptide can serve as a control substrate in peptide phosphorylation assays for the detection of SnRK1 activity in Arabidopsis thaliana, potato tuber tissues, and during SnRK1 purification.
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- No. CAS: 163560-19-8
- Fòrmula: C62H115N27O17S
- Peso molecular:1542.81
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
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SnRK1 |
AMARA peptide is potently phosphorylated by purified spinach SnRK1 in vitro with a specific activity of 142 nmol/min/mg[1].
AMARA peptide is phosphorylated by crude soluble protein extracts from liquid-cultured Arabidopsis thaliana (Col-4) seedlings with a calcium-independent activity of 2.49 nmol/min/mg, 90% of which is attributable to SnRK1, plus a small additional calcium-dependent activity[1].
The AMARA peptide functions as an improved synthetic substrate for plant SnRK1 compared to the SAMS peptide[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 163560-19-8
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Peso molecular 1542.81
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Fòrmula C62H115N27O17S
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Sequence
Ala-Met-Ala-Arg-Ala-Ala-Ser-Ala-Ala-Ala-Leu-Ala-Arg-Arg-Arg
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Sequence Shortening
AMARAASAAALARRR
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvente y solubilidad
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
|---|---|---|
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
|---|---|
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Pureza y Documentación
Referencias
[2]. Halford NG, et al. Carbon metabolite sensing and signalling. Plant biotechnology journal. 2003 Nov;1(6):381-98. [Content Brief]
[3]. Halford NG, et al.. SNF1-related protein kinases: global regulators of carbon metabolism in plants? Plant Mol Biol. 1998 Jul;37(5):735-48. [Content Brief]
[4]. Halford NG, et al. Metabolic signalling and carbon partitioning: role of Snf1-related (SnRK1) protein kinase. Journal of experimental botany. 2003 Jan;54(382):467-75. [Content Brief]
[5]. Wu X, et al. Hydrogen sulfide promotes lateral root formation in peach through persulfidation of SnRK1α kinase. Plant Biotechnol J. 2025 Oct;23(10):4395-4411. [Content Brief]
[6]. McKibbin RS, et al. Production of high-starch, low-glucose potatoes through over-expression of the metabolic regulator SnRK1. Plant Biotechnol J. 2006 Jul;4(4):409-18. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AMARA peptide Related Classifications
HY-P1576 Related Classifications
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