Referencia número: HY-102048

STAT5-IN-2

2 Cited Publications

Revisión del cliente

Based on 2 publication(s) in Google Scholar

STAT5-IN-2 is a STAT5 inhibitor, extracted from reference 1, example 17f. STAT5-IN-2 has potent antileukemic effect.

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Pureza: 98.90%
No. CAS: 2111834-61-6
Fòrmula: C₂₆H₂₇N₃O
Peso molecular:397.51

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

STAT5-IN-2 Chemical Structure

No. CAS: 2111834-61-6

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Actividad biológica
- Descripciòn
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (2)
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

STAT5-IN-2 is a STAT5 inhibitor, extracted from reference 1, example 17f. STAT5-IN-2 has potent antileukemic effect^[1].

IC₅₀ & Target^[1]

STAT5

9 μM (EC₅₀, in K562 cells)

STAT5

5 μM (EC₅₀, in KU812 cells )

Ver todos los productos específicos de isoformas STAT :

In Vitro

STAT5-IN-2 has EC₅₀ values of 9 μM and 5 μM in K562 and KU812 cells, respectively^[1].
STAT5-IN-2 (100 nM-50 μM; 48 hours) has EC₅₀ values of 2.6 μM and 3.5 μM in KG1a and MV-4-11 cells, respectively^[1].
STAT5-IN-2 (10 μM; 48 hours) induces apoptosis in all cell lines (K562, KU812, KG1a and MV-4-11 cells)^[1].
STAT5-IN-2 (10 μM; 24 hours) blocks phosphorylation of STAT5^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

KG1a and MV-4-11 cell lines
Concentration:

100 nM, 10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time:

48 hours
Result:

Inhibited cell growth in KG1a and MV-4-11 cell lines

Apoptosis Analysis^[1]

Cell Line:

K562, KU812, KG1a and MV-4-11 cells
Concentration:

10 µM
Incubation Time:

48 hours
Result:

Significantly increased the number of apoptotic cells.

Western Blot Analysis^[1]

Cell Line:

KG1a, MV-4-11 and KU812 cells
Concentration:

10 µM
Incubation Time:

24 hours
Result:

Inhibited phosphorylation of STAT5 compared to no influence on phosphorylation level of STAT3, Akt and Erk1/2.

Chemical Information

No. CAS 2111834-61-6
Appearance Solid
Peso molecular 397.51
Fòrmula C₂₆H₂₇N₃O
Color Off-white to light yellow
SMILES

CC(CCN1CCOC2=CC(C=CN3)=C3C=C2)(C)C4=C1C=CC(C5=CC=CN=C5)=C4
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

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Journal Impact Factor

J Exp Med

Transcription factor Zhx2 restricts NK cell maturation and suppresses their antitumor immunity. [Abstract]

2021 Sep 6;218(9):e20210009. PMID: 34279541
J Immunol

Tumor Environment Promotes Lnc57Rik-Mediated Suppressive Function of Myeloid-Derived Suppressor Cells. [Abstract]

2022 Oct 1;209(7):1401-1413. PMID: 36038289

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (251.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5157 mL

12.5783 mL

25.1566 mL

5 mM

0.5031 mL

2.5157 mL

5.0313 mL

10 mM

0.2516 mL

1.2578 mL

2.5157 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (275 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Ludovic Juen, et al. New Inhibitor Targeting Signal Transducer and Activator of Transcription 5 (STAT5) Signaling in Myeloid Leukemias. J Med Chem. 2017 Jul 27;60(14):6119-6136. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5157 mL	12.5783 mL	25.1566 mL	62.8915 mL
	5 mM	0.5031 mL	2.5157 mL	5.0313 mL	12.5783 mL
	10 mM	0.2516 mL	1.2578 mL	2.5157 mL	6.2891 mL
	15 mM	0.1677 mL	0.8386 mL	1.6771 mL	4.1928 mL
	20 mM	0.1258 mL	0.6289 mL	1.2578 mL	3.1446 mL
	25 mM	0.1006 mL	0.5031 mL	1.0063 mL	2.5157 mL
	30 mM	0.0839 mL	0.4193 mL	0.8386 mL	2.0964 mL
	40 mM	0.0629 mL	0.3145 mL	0.6289 mL	1.5723 mL
	50 mM	0.0503 mL	0.2516 mL	0.5031 mL	1.2578 mL
	60 mM	0.0419 mL	0.2096 mL	0.4193 mL	1.0482 mL
	80 mM	0.0314 mL	0.1572 mL	0.3145 mL	0.7861 mL
	100 mM	0.0252 mL	0.1258 mL	0.2516 mL	0.6289 mL

Keywords

STAT5-IN-2

2 Cited Publications

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STAT5-IN-2

Apoptosis Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Targeted Diversity Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) STAT5-IN-2

Ver todos los productos específicos de isoformas STAT

Actividad biológica

Chemical Information

Publications (2)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

STAT5-IN-2

Apoptosis Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Targeted Diversity Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) STAT5-IN-2

Ver todos los productos específicos de isoformas STAT

Actividad biológica

Chemical Information

Publications (2)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

STAT5-IN-2 Related Classifications

HY-102048 Related Classifications