alpha-Cyperone
Based on 3 publication(s) in Google Scholar
alpha-Cyperone (α-Cyperone) is associated with the down-regulation of COX-2, IL-6, Nck-2, Cdc42 and Rac1, resulting in reduction of inflammation, which would be highly beneficial for treatment of inflammatory diseases such as AD.
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- Pureza : 99.68%
- No. CAS: 473-08-5
- Fòrmula: C15H22O
- Peso molecular:218.33
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Almacenamiento:
-20°C, protect from light
* The compound is unstable in solutions, freshly prepared is recommended.
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Publications Citing Use of MedChemExpress (MCE) alpha-Cyperone
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In Vitro
The anti-inflammatory activity of alpha-Cyperone (α-Cyperone) is associated with the down-regulation of COX-2 and IL-6 via the negative regulation of the NFκB pathway in LPS-stimulated RAW 264.7 cells[1].
alpha-Cyperone (α-Cyperone) binds and interacts with tubulin and is capable of distinctly destabilizing microtubule polymerization. The effect of this interaction could result in reduction of inflammation which would be highly beneficial for treatment of inflammatory diseases such as AD. One microliter of alpha-Cyperone was dissolved in DMSO (1:1 v/v) and it was further diluted in double distilled water (ddH2O) to a final volume of 20 microliter[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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No. CAS 473-08-5
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Appearance Liquid (Density: 0.9946 g/cm3)
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Peso molecular 218.33
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Fòrmula C15H22O
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Color Colorless to light yellow
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SMILES
O=C1C(C)=C2C[C@H](C(C)=C)CC[C@@]2(C)CC1
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Synonyms
α-Cyperone; (+)-α-Cyperone
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, protect from light
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (3)
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Journal Impact Factor
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Most Recent
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Immunopharmacol Immunotoxicol
Alpha-cyperone ameliorates renal fibrosis and inflammation in mice with chronic kidney disease via NF-κB and Akt/Nrf2/HO-1 pathways. [Abstract]2025 Aug;47(4):460-470. PMID: 40415449 -
Immunopharmacol Immunotoxicol
α-Cyperone ameliorates depression in mammary gland hyperplasia and chronic unpredictable mild stress rat by regulating hormone, inflammation, and oxidative stress. [Abstract]2023 Feb;45(1):73-82. PMID: 36053011 -
Aging (Albany NY)
α-Cyperone (CYP) down-regulates NF-κB and MAPKs signaling, attenuating inflammation and extracellular matrix degradation in chondrocytes, to ameliorate osteoarthritis in mice. [Abstract]2021 Jul 8;13(13):17690-17706. PMID: 34237707
alpha-Cyperone purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2021 Jul 8;13(13):17690-17706. [Abstract]
alpha-Cyperone (CYP)’s cytotoxicity of in an increasing concentration (0, 0.75, 1.5, 3, 6 μM) on chondrocytes as measured by CCK-8 assay after a 24 or 48-hour incubation.
alpha-Cyperone purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2021 Jul 8;13(13):17690-17706. [Abstract]
Expression of INOS, COX-2, TNF-α and IL-6 genes were determined by real-time PCR treated with alpha-Cyperone (CYP) (0, 0.75, 1.5, 3 μM).
alpha-Cyperone purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2021 Jul 8;13(13):17690-17706. [Abstract]
Levels of INOS, COX-2, TNF-α and IL-6 inflammatory mediators in rat chondrocytes were detected by western blot assay treated with alpha-Cyperone (CYP) (0, 0.75, 1.5, 3 μM).
alpha-Cyperone purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2021 Jul 8;13(13):17690-17706. [Abstract]
The type II collagen was assayed through cell immunofluorescence combined with DAPI staining for nuclei treated with alpha-Cyperone (CYP) (0, 0.75, 1.5, 3 μM).
alpha-Cyperone purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2021 Jul 8;13(13):17690-17706. [Abstract]
Immunohistochemical staining of collagen II and MMP-13 expression in the cartilage samples treated with alpha-Cyperone (CYP) (10 mg/kg, i.p.).
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (458.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * The compound is unstable in solutions, freshly prepared is recommended.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Jung, S. H. et al. alpha-Cyperone, isolated from the rhizomes of Cyperus rotundus, inhibits LPS-induced COX-2 expression and PGE2 production through the negative regulation of NFkappaB signalling in RAW 264.7 cells. Journal of ethnopharmacology 147, 208-214, doi:10.1016/j.jep.2013.02.034 (2013). [Content Brief]
[2]. Azimi, A. et al. alpha-Cyperone of Cyperus rotundus is an effective candidate for reduction of inflammation by destabilization of microtubule fibers in brain. Journal of ethnopharmacology, doi:10.1016/j.jep.2016.06.058 (2016). [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.5802 mL | 22.9011 mL | 45.8022 mL | 114.5056 mL |
| 5 mM | 0.9160 mL | 4.5802 mL | 9.1604 mL | 22.9011 mL | |
| 10 mM | 0.4580 mL | 2.2901 mL | 4.5802 mL | 11.4506 mL | |
| 15 mM | 0.3053 mL | 1.5267 mL | 3.0535 mL | 7.6337 mL | |
| 20 mM | 0.2290 mL | 1.1451 mL | 2.2901 mL | 5.7253 mL | |
| 25 mM | 0.1832 mL | 0.9160 mL | 1.8321 mL | 4.5802 mL | |
| 30 mM | 0.1527 mL | 0.7634 mL | 1.5267 mL | 3.8169 mL | |
| 40 mM | 0.1145 mL | 0.5725 mL | 1.1451 mL | 2.8626 mL | |
| 50 mM | 0.0916 mL | 0.4580 mL | 0.9160 mL | 2.2901 mL | |
| 60 mM | 0.0763 mL | 0.3817 mL | 0.7634 mL | 1.9084 mL | |
| 80 mM | 0.0573 mL | 0.2863 mL | 0.5725 mL | 1.4313 mL | |
| 100 mM | 0.0458 mL | 0.2290 mL | 0.4580 mL | 1.1451 mL |
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