CK156
Based on 1 Customer Validation
CK156, a chemical probe, is a highly selective death-associated protein kinase (DAPK) inhibitor with IC50s of 182 nM,34 μM, and 39 μM in the DRAK1 NanoBRET assay for DRAK1, CK2a1, and CK2a2, respectively. CK156 can be used for the research of autoimmune and inflammatory diseases.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.60%
- No. CAS: 2910938-59-7
- Fòrmula: C21H25N5O3
- Peso molecular:395.45
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| D-247MG | EC50 |
2.75 μM
Compound: 34; CK156
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Antiproliferative activity against human D-247MG cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis
Antiproliferative activity against human D-247MG cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis
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[PMID: 35608370] |
| HEK-293T | EC50 |
>10 μM
Compound: 34; CK156
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Cytotoxicity against human HEK293T cells assessed as increase in total apoptotic cells incubated for 36 hrs by Annexin V staining based method
Cytotoxicity against human HEK293T cells assessed as increase in total apoptotic cells incubated for 36 hrs by Annexin V staining based method
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[PMID: 35608370] |
| LN-18 | EC50 |
11.23 μM
Compound: 34; CK156
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Antiproliferative activity against human LN-18 cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis
Antiproliferative activity against human LN-18 cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis
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[PMID: 35608370] |
| LN-229 | EC50 |
9.2 μM
Compound: 34; CK156
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Antiproliferative activity against human LN-229 cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis
Antiproliferative activity against human LN-229 cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis
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[PMID: 35608370] |
| T98G | EC50 |
10.41 μM
Compound: 34; CK156
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Antiproliferative activity against human T98G cells assessed as reduction in cell viability measured after 1 to 7 days by resazurin dye based fluorimetric analysis
Antiproliferative activity against human T98G cells assessed as reduction in cell viability measured after 1 to 7 days by resazurin dye based fluorimetric analysis
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[PMID: 35608370] |
Chemical Information
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No. CAS 2910938-59-7
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Appearance Solid
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Peso molecular 395.45
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Fòrmula C21H25N5O3
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Color White to light yellow
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SMILES
O=C(C1=CC=C2C=C1OCCOCCNC3=NC4=C2C=NN4C=C3)NC(C)(C)C
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (252.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.32 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.32 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5288 mL | 12.6438 mL | 25.2876 mL | 63.2191 mL |
| 5 mM | 0.5058 mL | 2.5288 mL | 5.0575 mL | 12.6438 mL | |
| 10 mM | 0.2529 mL | 1.2644 mL | 2.5288 mL | 6.3219 mL | |
| 15 mM | 0.1686 mL | 0.8429 mL | 1.6858 mL | 4.2146 mL | |
| 20 mM | 0.1264 mL | 0.6322 mL | 1.2644 mL | 3.1610 mL | |
| 25 mM | 0.1012 mL | 0.5058 mL | 1.0115 mL | 2.5288 mL | |
| 30 mM | 0.0843 mL | 0.4215 mL | 0.8429 mL | 2.1073 mL | |
| 40 mM | 0.0632 mL | 0.3161 mL | 0.6322 mL | 1.5805 mL | |
| 50 mM | 0.0506 mL | 0.2529 mL | 0.5058 mL | 1.2644 mL | |
| 60 mM | 0.0421 mL | 0.2107 mL | 0.4215 mL | 1.0537 mL | |
| 80 mM | 0.0316 mL | 0.1580 mL | 0.3161 mL | 0.7902 mL | |
| 100 mM | 0.0253 mL | 0.1264 mL | 0.2529 mL | 0.6322 mL |
CK156 Related Classifications
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