Referencia número: HY-137984

Cnicin

Name: Cnicin
Brand: MedChemExpress
SKU: HY-137984
Price: 144.00 USD
Availability: InStock
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Cnicin is an orally bioavailable sesquiterpene lactone. Cnicin has antibacterial and antiproliferative properties and induces apoptosis in primary myeloma cells. Cnicin also exhibits activity against SARS-CoV-2. Cnicin inhibits the viral replication of SARS CoV-2 with an IC₅₀ of 1.18 μg/mL. Cnicin can promote functional nerve regeneration.

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Pureza : 99.14%
No. CAS: 24394-09-0
Fòrmula: C₂₀H₂₆O₇
Peso molecular:378.42

Almacenamiento:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Cnicin Chemical Structure

No. CAS: 24394-09-0

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Top Publications Citing

Use of MCE Products

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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Actividad biológica
- Descripciòn
- In Vitro
- In Vivo
Chemical Information
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

In Vitro

Cnicin (0-10 μM, 72 h) inhibits the proliferation of multiple myeloma cells, with an IC₅₀ of 3-13 μM, and induces apoptosis^[1].
Cnicin (2 μM, 0-12 h) induces cell death, partly depending on caspase^[1].
Cnicin (0.5-5 nM, 4 days) has significant effects on promoting nerve regeneration^[3].
Cnicin demonstrates strong inhibitory activity on NF-κB, SP-1, and iNOS in PMA (HY-18739) induced SW1353 cells, with IC₅₀ values of 1.8, 16, and 6.5 µM^[4].
Cnicin (50 μg) has a zone of inhibition for Gram-positive bacteria ranging from 14-17 mm, and for Gram-negative bacteria, it ranges from 10-20 mm^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

LP-1, NCI, OPM-2, RPMI, U266, MM1.S
Concentration:

0, 2, 4, 6, 8, 10 μM
Incubation Time:

72 h
Result:

Inhibited cell proliferation.

Western Blot Analysis^[1]

Cell Line:

NCI-H929
Concentration:

2 μM
Incubation Time:

0, 3, 6, 9, 12 h
Result:

Induced the activation of Caspase, downregulation of Caspase 3, cleavage of Caspase 3 and cleaved Caspases 8 and 9.

In Vivo

Cnicin (0.28-28 μg/kg, intravenous injection, once a day, 3-12 days) effectively promotes nerve regeneration and accelerates functional recovery in mice^[3].
Pharmacokinetic parameters of a single intravenous or oral administration of 0.8 mg Cnicin in rats^[3]

Parameters	Estimate	RES (%)
K_a (1/min)	0.0321	18.4
CL (1/min)	0.00101	10.7
V_C (l)	0.00745	8.9
F1 (%)	0.847	14.6
Q_p (1/min)	0.00298	5.0
V_p (l)	0.00985	12.5
Lag time (min)	5.02	13.6
CL (%)	17.2 (13.0)	31.6
V_c (%)	29.0 (32.0)	27.8
Additive error (%)	4.76 (6.4)	12.1

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Nerve injury mice^[3]
Dosage:

0.28, 2.8, 28 µg/kg; daily; 3-12 days
Administration:

Intravenous injection
Result:

Improved axon regeneration in sensory neurons and increased axon length in sensory, motor, and sympathetic neurons.

Related Protocols :

Chemical Information

No. CAS 24394-09-0
Appearance Solid
Peso molecular 378.42
Fòrmula C₂₀H₂₆O₇
Color White to off-white
SMILES

O=C(C([C@@H](O)CO)=C)O[C@@H](C/C(C)=C/CC1)[C@](C2=C)([H])[C@@](OC2=O)([H])/C=C1\CO
Structure Classification
- Terpenoids
- Sesquiterpenes
Initial Source
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (264.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6426 mL

13.2128 mL

26.4257 mL

5 mM

0.5285 mL

2.6426 mL

5.2851 mL

10 mM

0.2643 mL

1.3213 mL

2.6426 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (286 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Jöhrer K, et al. Antimyeloma activity of the sesquiterpene lactone cnicin: impact on Pim-2 kinase as a novel therapeutic target. J Mol Med (Berl). 2012 Jun;90(6):681-93. [Content Brief]

[2]. Alhadrami HA, et al. Cnicin as an Anti-SARS-CoV-2: An Integrated In Silico and In Vitro Approach for the Rapid Identification of Potential COVID-19 Therapeutics. Antibiotics (Basel). 2021 May 7;10(5):542. [Content Brief]

[3]. Philipp Gobrecht, et al. Cnicin promotes functional nerve regeneration. Phytomedicine. 2024 Jul:129:155641. [Content Brief]

[4]. Sura Baykan Erel, et al. Secondary metabolites of Centaurea calolepis and evaluation of cnicin for anti-inflammatory, antioxidant, and cytotoxic activities. Pharm Biol. 2011 Aug;49(8):840-9. [Content Brief]

[5]. Sandra M Bach, et al. Antibacterial and cytotoxic activities of the sesquiterpene lactones cnicin and onopordopicrin. Nat Prod Commun. 2011 Feb;6(2):163-6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6426 mL	13.2128 mL	26.4257 mL	66.0642 mL
	5 mM	0.5285 mL	2.6426 mL	5.2851 mL	13.2128 mL
	10 mM	0.2643 mL	1.3213 mL	2.6426 mL	6.6064 mL
	15 mM	0.1762 mL	0.8809 mL	1.7617 mL	4.4043 mL
	20 mM	0.1321 mL	0.6606 mL	1.3213 mL	3.3032 mL
	25 mM	0.1057 mL	0.5285 mL	1.0570 mL	2.6426 mL
	30 mM	0.0881 mL	0.4404 mL	0.8809 mL	2.2021 mL
	40 mM	0.0661 mL	0.3303 mL	0.6606 mL	1.6516 mL
	50 mM	0.0529 mL	0.2643 mL	0.5285 mL	1.3213 mL
	60 mM	0.0440 mL	0.2202 mL	0.4404 mL	1.1011 mL
	80 mM	0.0330 mL	0.1652 mL	0.3303 mL	0.8258 mL
	100 mM	0.0264 mL	0.1321 mL	0.2643 mL	0.6606 mL

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Cnicin