JD-13
JD-13 is a HPV-1 inhibitor and anti-proliferative agent against cancer cells. JD-13 downregulates the expression of HSV-1 DNA polymerase-related genes UL30 and UL42, and inhibits viral replication. JD-13 induces apoptosis (apoptosis) in colorectal cancer cells and suppresses their colony formation. JD-13 can be applied to the research of herpes simplex virus infection and related diseases including colorectal cancer, lung cancer, esophageal cancer and breast cancer.
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- No. CAS: 2969172-58-3
- Fòrmula: C25H32N4O3
- Peso molecular:436.55
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Actividad biológica
JD-13 (0.125-0.5 μM; 72 h) potently inhibits acyclovir-resistant herpes simplex virus type 1 (HSV-1-153) infection in Vero cells, with near-complete inhibition at concentrations of 0.125, 0.25, and 0.5 μM[1].
JD-13 (0.125-0.5 μM; 24 h) effectively reduces herpes simplex virus type 1 genomic DNA copy numbers in Vero cells, with stronger activity than acyclovir at concentrations of 0.125, 0.25, and 0.5 μM after 24 hours of incubation[1].
JD-13 (0.5 μM) most potently inhibits herpes simplex virus type 1 protein expression in Vero cells when administered within the first 6 hours of infection[1].
JD-13 (0.5 μM; 2-6 h) downregulates the expression of herpes simplex virus type 1 DNA polymerase-related genes UL30 and UL42 in Vero cells, with significant inhibition observed at 4 hours for UL42 and 6 hours for both UL30 and UL42[1].
JD-13 (20-80 nM; 24 h) inhibits the colony formation ability of HCT116 and SW620 human colon cancer cells after a 24-hour treatment followed by 14 days of drug-free culture[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Vero cells
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Concentration:0.5 μM
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Incubation Time:24 h (added at 0 h post-infection); 21 h (added at 3 h post-infection); 18 h (added at 6 h post-infection); 15 h (added at 9 h post-infection); 12 h (added at 12 h post-infection)
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Result:Showed the strongest inhibitory effect on the expression of HSV-1 proteins gB and ICP0 when added within 6 hours post-infection.
Exhibited weakened inhibitory effect when added at 9 or 12 hours post-infection.
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Cell Line:Vero cells
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Concentration:0.5 μM
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Incubation Time:2 h; 4 h; 6 h
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Result:Had no significant effect on the expression of UL30 and UL42 at 2 hours post-treatment.
Significantly downregulated UL42 expression at 4 hours post-treatment.
Significantly downregulated both UL30 and UL42 expression at 6 hours post-treatment.
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Cell Line:HCT116, SW620, A549, Eca109, MDA-MB-231
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Concentration:Variable concentrations
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Incubation Time:48 h
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Result:Inhibited the viability of HCT116, SW620, A549, Eca109, and MDA-MB-231 cells in a concentration-dependent manner.
Had IC50 values of 35.49 nM for HCT116, 24.64 nM for SW620, 36.19 nM for A549, 35.82 nM for Eca109, and 52.14 nM for MDA-MB-231.
Showed significantly lower IC50 values than the reference compound JD-02.
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Cell Line:HCT116, SW620
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Concentration:20-80 nM
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Incubation Time:24 h (followed by 14 days of drug-free culture)
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Result:Significantly reduced the size and number of cell colonies formed by HCT116 and SW620 cells compared to the control group.
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Cell Line:HCT116, SW620
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Concentration:Low, medium, and high doses
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Incubation Time:48 h
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Result:Induced typical apoptotic characteristics including cell shrinkage, cytoplasmic condensation, highly condensed and marginalized nuclear chromatin, fragmented nuclei, and apoptotic bodies compared to the blank control group.
Increased the number of cells with strong blue fluorescent apoptotic nuclei in a concentration-dependent manner.
Chemical Information
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No. CAS 2969172-58-3
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Peso molecular 436.55
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Fòrmula C25H32N4O3
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SMILES
O=C(C1=CC=C(N2N=C(C)C3=C2CC(C)(C)CC3=O)C=C1NC45CCC(CC4)(CC5)O)N
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
JD-13 Related Classifications
HY-184394 Related Classifications
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