L82
Based on 1 Customer Validation
L82 is a selective and uncompetitive DNA ligase 1 (DNA Lig1) inhibitor (hLig1 IC50=12 μM). L82 shows anti-proliferative activity to breast cancer cells.
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- Pureza : 98.89%
- No. CAS: 329227-30-7
- Fòrmula: C11H8ClN5O4
- Peso molecular:309.67
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
IC50 & Target
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DNA Ligase |
In Vitro
L82 (0-50 μM; 6 d) shows anti-proliferative activity to breast cancer cells[2].
L82 (50 μM; 0-48 h) shows cytostatic activity due to activation of the G1/S checkpoint in MCF7 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF10A, MCF7, HCT116, and HeLa cells
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Concentration:0-50 μM
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Incubation Time:6 days
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Result:Reduced the proliferation of a normal breast epithelial cell line MCF10A and the breast cancer cell lines MCF7, HeLa and HCT116, in a concentration-dependent manner.
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Cell Line:MCF7 cells
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Concentration:50 μM
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Incubation Time:0-48 hours
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Result:Showed a transient accumulation of cells at G2/M after 12 h, then showed an accumulation at G0/G1 that peaked after 24 h.
Decreased in the S phase cell in accompany with the increase in the G0/G1 phase.
Chemical Information
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No. CAS 329227-30-7
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Appearance Solid
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Peso molecular 309.67
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Fòrmula C11H8ClN5O4
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Color Yellow to orange
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SMILES
O=C1NN=CC(N/N=C/C2=CC=C(C([N+]([O-])=O)=C2)O)=C1Cl
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 33.33 mg/mL (107.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Howes TRL, et al. Structure-activity relationships among DNA ligase inhibitors: Characterization of a selective uncompetitive DNA ligase I inhibitor. DNA Repair (Amst). 2017 Dec;60:29-39. [Content Brief]
[2]. Chen X, et al. Rational design of human DNA ligase inhibitors that target cellular DNA replication and repair. Cancer Res. 2008 May 1;68(9):3169-77. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2292 mL | 16.1462 mL | 32.2924 mL | 80.7311 mL |
| 5 mM | 0.6458 mL | 3.2292 mL | 6.4585 mL | 16.1462 mL | |
| 10 mM | 0.3229 mL | 1.6146 mL | 3.2292 mL | 8.0731 mL | |
| 15 mM | 0.2153 mL | 1.0764 mL | 2.1528 mL | 5.3821 mL | |
| 20 mM | 0.1615 mL | 0.8073 mL | 1.6146 mL | 4.0366 mL | |
| 25 mM | 0.1292 mL | 0.6458 mL | 1.2917 mL | 3.2292 mL | |
| 30 mM | 0.1076 mL | 0.5382 mL | 1.0764 mL | 2.6910 mL | |
| 40 mM | 0.0807 mL | 0.4037 mL | 0.8073 mL | 2.0183 mL | |
| 50 mM | 0.0646 mL | 0.3229 mL | 0.6458 mL | 1.6146 mL | |
| 60 mM | 0.0538 mL | 0.2691 mL | 0.5382 mL | 1.3455 mL | |
| 80 mM | 0.0404 mL | 0.2018 mL | 0.4037 mL | 1.0091 mL | |
| 100 mM | 0.0323 mL | 0.1615 mL | 0.3229 mL | 0.8073 mL |
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