Micranoic acid B
Micranoic acid B is a terpenoid compound. Micranoic acid B exhibits cytotoxicity against cancer cells and shows comparable activity against multidrug-resistant and parental cancer cell lines. Micranoic acid B can be used for research on lung cancer, prostate cancer, and nasopharyngeal epidermoid carcinoma.
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- No. CAS: 659738-09-7
- Fòrmula: C22H32O3
- Peso molecular:344.50
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | GI50 |
12.79 μM
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Cytotoxicity against human lung carcinoma A549 cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay.
Cytotoxicity against human lung carcinoma A549 cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay.
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23883077 |
| PC-3 | GI50 |
11.83 μM
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Cytotoxicity against human prostate carcinoma PC-3 cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay.
Cytotoxicity against human prostate carcinoma PC-3 cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay.
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23883077 |
| KB | GI50 |
12.62 μM
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Cytotoxicity against human epidermoid carcinoma of the nasopharynx KB cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay.
Cytotoxicity against human epidermoid carcinoma of the nasopharynx KB cells assessed as 50% inhibition of cell growth incubated for 72 hrs by sulforhodamine B (SRB) colorimetric assay.
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23883077 |
In Vitro
Micranoic acid B (compound 5) (72 h) exhibits moderate cytotoxicity against A549, PC-3, KB and KBvin human cancer cell lines with GI50 values of 11.83-12.79 μM[4].
Micranoic acid B (compound 93) is a norcycloartane triterpenoid isolated from Schisandra micrantha that features an octanortriterpenoid backbone lacking the entire C-17 side chain[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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No. CAS 659738-09-7
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Peso molecular 344.50
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Fòrmula C22H32O3
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SMILES
C(CC(O)=O)[C@]12[C@]3([C@]([C@@]4(C)[C@](C)(CC3)C(=O)CC4)(CC[C@H]1C(C)=C)[H])C2
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureza y Documentación
Referencias
[2]. Xiao WL, et al. Triterpenoids from the Schisandraceae family. Natural product reports. 2008 Oct;25(5):871-91. [Content Brief]
[4]. Lu Y, et al. Cytotoxic and potential anticancer constituents from the stems of Schisandra pubescens. Pharmaceutical biology. 2013 Sep;51(9):1204-7. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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