Pitstop 2
Based on 35 publication(s) in Google Scholar
Pitstop 2 is a clathrin inhibitor which inhibits clathrin-mediated endocytosis (CME) by associating with the terminal domain of clathrin. Pitstop 2 has the potential for anti-cancer research.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.42%
- No. CAS: 1419320-73-2
- Fòrmula: C20H13BrN2O3S2
- Peso molecular:473.36
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
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Publications Citing Use of MedChemExpress (MCE) Pitstop 2
More- Cell. 2026 Apr 2;189(7):1923-1941.e26. [Abstract]
- Mol Cancer. 2026 Feb 10;25(1):73. [Abstract]
- Immunity. 2022 Aug 9;55(8):1370-1385.e8. [Abstract]
- Adv Mater. 2026 Jun;38(33):e21108. [Abstract]
- Nat Immunol. 2025 Oct;26(10):1673-1685. [Abstract]
- Bone Res. 2025 Oct 20;13(1):88. [Abstract]
- Adv Funct Mater. 2025 Aug 3.
- Adv Funct Mater. 2024 May 1.
- Nat Commun. 2025 May 27;16(1):4887. [Abstract]
- Nat Commun. 2023 Mar 15;14(1):1437. [Abstract]
- ACS Nano. 2024 Feb 27;18(8):6463-6476. [Abstract]
- Nat Chem Biol. 2026 Feb 13. [Abstract]
- J Nanobiotechnology. 2025 Nov 23;23(1):797. [Abstract]
- Adv Sci (Weinh). 2025 May;12(17):e2417312. [Abstract]
- Carbohydr Polym. 2023 Apr 1:305:120533. [Abstract]
- J Pharm Anal. 2025 Jul;15(7):101182. [Abstract]
- Proc Natl Acad Sci U S A. 2025 Mar 25;122(12):e2502064122. [Abstract]
- Int J Biol Macromol. 2024 Aug 5;277(Pt 3):134517. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2026 Jan 7;20(5):101721. [Abstract]
- Environ Health. 2025 Nov 6.
- Small Sci. 2025 Dec 15.
- Bioeng Transl Med. 2022 Jul 30;8(1):e10377. [Abstract]
- Commun Biol. 2026 May 11. [Abstract]
- Commun Biol. 2024 Dec 31;7(1):1719. [Abstract]
- Biol Res. 2023 Mar 11;56(1):10. [Abstract]
- Aquaculture. 2026 May 4;623:744110.
- Antiviral Res. 2026 May:249:106403. [Abstract]
- J Biol Chem. 2026 Apr;302(4):111345. [Abstract]
- J Virol. 2025 Aug 25:e0022125. [Abstract]
- J Cell Sci. 2024 Oct 1;137(19):jcs262181. [Abstract]
- Traffic. 2023 Jan;24(1):20-33. [Abstract]
- Icahn School of Medicine at Mount Sinai. 2026.
- bioRxiv. 2026 Mar 12.
- Biomed Pharmacother. 2025 Dec:193:118716. [Abstract]
- Research Square Preprint. 2021 Oct.
Customer Validation & Images
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IF
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WB
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Cell Imaging/Staining
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RT-PCR
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Flow Cytometry
Actividad biológica
Pitstop 2 (20-40 μM; 30 min) inhibits the endocytosis of transferrin in J774A.1 macrophages and does not compromise cell viability[1].
Pitstop 2 (20-40 μM; 30 min) does not affect the internalization of cholera toxin B in J774A.1 macrophages[1].
Pitstop 2 (0.001-100 μM; 6 h) inhibits the mitotic spindle and impairs mitotic progression in HeLa cells[2].
Pitstop 2 (1-30 μM; 24 h) induces apoptosis and inhibits cell growth in dividing cancer cells[2].
Pitstop 2 (1-30 μM; 48 h) does not affect the growth and viability of non-tumourigenic NIH3T3 fibroblasts[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:1, 3, 10, 30 μM
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Incubation Time:24 hours
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Result:Reduced the total number of viable HeLa cells markedly in a dose-dependent manner.
Chemical Information
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No. CAS 1419320-73-2
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Appearance Solid
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Peso molecular 473.36
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Fòrmula C20H13BrN2O3S2
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Color Yellow to brown
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SMILES
O=S(C1=C2C=CC=CC2=CC=C1)(NC(S/C3=C\C4=CC=C(Br)C=C4)=NC3=O)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
Publications (35)
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Journal Impact Factor
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Most Recent
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Cell
2026 Apr 2;189(7):1923-1941.e26. PMID: 41785850 -
Mol Cancer
Tumour-infiltrating microplastics disrupt the JAK-STAT-microbiota axis to promote immunotherapy resistance in colorectal cancer. [Abstract]2026 Feb 10;25(1):73. PMID: 41664174 -
Immunity
Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling. [Abstract]2022 Aug 9;55(8):1370-1385.e8. PMID: 35835107 -
Adv Mater
Conformationally Variable Peptides Trap and Detoxify Ox-LDL in Plaques for Attenuating Atherosclerosis in Multiple Species. [Abstract]2026 Jun;38(33):e21108. PMID: 42116761 -
Nat Immunol
Type A cholesterol-dependent cytolysins translocate to the trans-Golgi network for NLRP3 inflammasome activation. [Abstract]2025 Oct;26(10):1673-1685. PMID: 40913097
Pitstop 2 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1673-1685. [Abstract]
Representative immunofluorescence images, quantification of rPFO colocalization with the TGN and TGN dispersion in HeLa cells stably expressing scFv-sfGFP pre-treated with pitstop 2 (20 µM) for 1 h before addition of 0.90 nM 10xGCN4-rPFO. Scale bar, 5 μm.
Pitstop 2 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1673-1685. [Abstract]
Immunoblots of HEK293T cells stably expressing NLRP3, ASC, and caspase-1 (Casp1) pre-treated with 20 µM pitstop 2 for 1 h before addition of 0.90 nM rPFO for 40 min.
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Bone Res
Pak4-mediated crosstalk between necroptotic macrophages and tendon stem/progenitor cells contributes to traumatic heterotopic ossification formation. [Abstract]2025 Oct 20;13(1):88. PMID: 41111094 -
Pitstop 2 purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Aug 3.
Typical confocal images of RAW 264.7 cells incubated with 100 μg/mL FITC/M-BSA@PSiNPs for 6 h, in the absence or presence of 50 μmol/L Pitstop 2, 100 μmol/L Methyl-β-cyclodextrin (MβCD), 50 μmol/L amiloride, or 1 μmol/L CytoD (scale bar = 40 μm).
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Nat Commun
Clustering of NLRP3 induced by membrane or protein scaffolds promotes inflammasome assembly. [Abstract]2025 May 27;16(1):4887. PMID: 40425567 -
Nat Commun
Mechano-boosting nanomedicine antitumour efficacy by blocking the reticuloendothelial system with stiff nanogels. [Abstract]2023 Mar 15;14(1):1437. PMID: 36918575
Pitstop 2 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Mar 15;14(1):1437. [Abstract]
Relative cellular uptake of Rhodamine-labeled nanogels with different stiffness after inhibited by Pitstop 2 (1 μM, 5 μM, 10 μM, 20 μM; 0.5 h) at different dosage in RAW 264.7 cells.
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ACS Nano
Uncovering the Importance of Ligand Mobility on Cellular Uptake of Nanoparticles: Insights from Experimental, Computational, and Theoretical Investigations. [Abstract]2024 Feb 27;18(8):6463-6476. PMID: 38346263 -
Nat Chem Biol
2026 Feb 13. PMID: 41688711 -
J Nanobiotechnology
Size control of lipid nanoparticles via simulation-based design of a microfluidic chip and its effect on mRNA delivery in vitro and in vivo. [Abstract]2025 Nov 23;23(1):797. PMID: 41276828 -
Adv Sci (Weinh)
Plac1+ Tumor Cell-Treg Interplay Supports Tumorigenesis and Progression of Head and Neck Cancer. [Abstract]2025 May;12(17):e2417312. PMID: 40056047 -
Carbohydr Polym
Innate immune receptors co-recognition of polysaccharides initiates multi-pathway synergistic immune response. [Abstract]2023 Apr 1:305:120533. PMID: 36737186 -
J Pharm Anal
Effect of cholesterol on distribution, cell uptake, and protein corona of lipid microspheres at sites of cardiovascular inflammatory injury. [Abstract]2025 Jul;15(7):101182. PMID: 40735031 -
Proc Natl Acad Sci U S A
MITD1 is a brain-specific interferon-inducible factor that inhibits flavivirus replication. [Abstract]2025 Mar 25;122(12):e2502064122. PMID: 40112111
Pitstop 2 purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2025 Mar 25;122(12):e2502064122. [Abstract]
WNV entry into MITD1-expressing cells, assessed by RT-qPCR; PitStop2 at 50 µM for 2 h was used as control.
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Int J Biol Macromol
Growth inhibition of pancreatic cancer by targeted delivery of gemcitabine via fucoidan-coated pH-sensitive liposomes. [Abstract]2024 Aug 5;277(Pt 3):134517. PMID: 39111497 -
Cell Mol Gastroenterol Hepatol
OGT-enriched Hepatocyte-derived Extracellular Vesicles Promote Capillarization of Liver Sinusoidal Endothelial Cells in Metabolic Dysfunction-associated Steatotic Liver Disease. [Abstract]2026 Jan 7;20(5):101721. PMID: 41513003 -
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Bioeng Transl Med
Nanoalbumin-prodrug conjugates prepared via a thiolation-and-conjugation method improve cancer chemotherapy and immune checkpoint blockade therapy by promoting CD8+ T-cell infiltration. [Abstract]2022 Jul 30;8(1):e10377. PMID: 36684090 -
Commun Biol
2026 May 11. PMID: 42115777 -
Commun Biol
Proteolysis targeting chimera (PROTAC)-driven antibody internalization of oncogenic cell surface receptors. [Abstract]2024 Dec 31;7(1):1719. PMID: 39741170
Pitstop 2 purchased from MedChemExpress. Usage Cited in: Commun Biol. 2024 Dec 31;7(1):1719. [Abstract]
BT-474 and SKBR3 cells were assayed for Tmab internalization in presence of Pitstop2 (30 μM and 15 μM; 0-50 min).
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Biol Res
DYRK1-mediated phosphorylation of endocytic components is required for extracellular lumen expansion in ascidian notochord. [Abstract]2023 Mar 11;56(1):10. PMID: 36899423 -
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Antiviral Res
Salinomycin inhibits orthopoxvirus infection in vitro and in vivo by blocking endosomal acidification. [Abstract]2026 May:249:106403. PMID: 41941992 -
J Biol Chem
2026 Apr;302(4):111345. PMID: 41791708 -
J Virol
SFTSV utilizes AXL/GAS6 for entry via PI3K-PLC-dependent macropinocytosis activated by AXL-kinase. [Abstract]2025 Aug 25:e0022125. PMID: 40853130 -
J Cell Sci
The Scribble-SGEF-Dlg1 complex regulates E-cadherin and ZO-1 stability, turnover and transcription in epithelial cells. [Abstract]2024 Oct 1;137(19):jcs262181. PMID: 39350674 -
Traffic
AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice. [Abstract]2023 Jan;24(1):20-33. PMID: 36412210 -
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Biomed Pharmacother
Drug target proteome profiling identifies HES1-driven mitotic catastrophe in ovarian serous carcinoma. [Abstract]2025 Dec:193:118716. PMID: 41202420 -
Solvente y solubilidad
DMSO : 50 mg/mL (105.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (281 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Kumar GA, et, al. Leishmania donovani Internalizes into Host Cells via Caveolin-mediated Endocytosis. Sci Rep. 2019 Sep 2; 9(1): 12636. [Content Brief]
[2]. Smith CM, et, al. Inhibition of clathrin by pitstop 2 activates the spindle assembly checkpoint and induces cell death in dividing HeLa cancer cells. Mol Cancer. 2013 Jan 17; 12:4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1126 mL | 10.5628 mL | 21.1256 mL | 52.8139 mL |
| 5 mM | 0.4225 mL | 2.1126 mL | 4.2251 mL | 10.5628 mL | |
| 10 mM | 0.2113 mL | 1.0563 mL | 2.1126 mL | 5.2814 mL | |
| 15 mM | 0.1408 mL | 0.7042 mL | 1.4084 mL | 3.5209 mL | |
| 20 mM | 0.1056 mL | 0.5281 mL | 1.0563 mL | 2.6407 mL | |
| 25 mM | 0.0845 mL | 0.4225 mL | 0.8450 mL | 2.1126 mL | |
| 30 mM | 0.0704 mL | 0.3521 mL | 0.7042 mL | 1.7605 mL | |
| 40 mM | 0.0528 mL | 0.2641 mL | 0.5281 mL | 1.3203 mL | |
| 50 mM | 0.0423 mL | 0.2113 mL | 0.4225 mL | 1.0563 mL | |
| 60 mM | 0.0352 mL | 0.1760 mL | 0.3521 mL | 0.8802 mL | |
| 80 mM | 0.0264 mL | 0.1320 mL | 0.2641 mL | 0.6602 mL | |
| 100 mM | 0.0211 mL | 0.1056 mL | 0.2113 mL | 0.5281 mL |
Pitstop 2 Related Classifications
HY-115604 Related Classifications
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