SSK1
Based on 4 publication(s) in Google Scholar
SSK1, a senescence-specific killing compound, is a β-galactosidase-targeted proagent attenuates inflammation. SSK1 is activated by lysosomal β-galactosidase and selectively killed senescent cells through the activation of p38 MAPK and induction of apoptosis.
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- Pureza : 99.75%
- No. CAS: 2629250-69-5
- Fòrmula: C31H34F2N4O18
- Peso molecular:788.61
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Almacenamiento:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Publications Citing Use of MedChemExpress (MCE) SSK1
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Actividad biológica
Descripciòn
In Vitro
SSK1 (0.5 μM; 12-72 hours) activates the phosphorylation levels of both p38 MAPK and MKK3/MKK6 in senescent cells. SSK1 kills senescent cells through the activation of the p38 MAPK signaling pathway. SSK1 is able to induce mitochondrial DNA damage in senescent cells[1].
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SSK1 (0.01-1 μM; 3 days) selectively and potently eliminates β-galactosidase-positive senescent cells within a wide therapeutic window[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary mouse fibroblasts
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Concentration:0.5 µM
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Incubation Time:12 hours, 24 hours, 36 hours, 48 hours, 72 hours
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Result:Both p38 MAPK and MKK3/MKK6 were activated by phosphorylation in senescent cells.
In Vivo
? In aged mice (20-month-old), SSK1 (0.5 mg/kg; 3 days every 2 weeks for 8 weeks) effectively clears senescent cells in different tissues, decreases the senescence- and age-associated gene signatures, attenuates low-grade local and systemic inflammation, and restores physical function[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mice (3-6-month-old) were subjected to transtracheal injection of Bleomycin[1]
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Dosage:0.5 mg/kg
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Administration:Intraperitoneally injection; two days every week; for four weeks
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Result:SSK1 significantly reduced the percentage of SA-β-gal-positive cells in lung by 3.8-fold compared with that in vehicle-treated lung-injured mice
Chemical Information
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No. CAS 2629250-69-5
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Appearance Solid
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Peso molecular 788.61
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Fòrmula C31H34F2N4O18
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Color White to off-white
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SMILES
CC(O[C@H]([C@H]([C@H]1OC(C)=O)OC(C)=O)[C@@H](O[C@@H]1COC(C)=O)OC(C=CC(COC(NC(C=CN2[C@](C(F)([C@@H]3O)F)([H])O[C@@H]3CO)=NC2=O)=O)=C4)=C4[N+]([O-])=O)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Cell Death Discov
2026 Mar 11. PMID: 41813667 -
Clin Epigenetics
KDM4C-mediated senescence defense is a targetable vulnerability in gastric cancer harboring TP53 mutations. [Abstract]2023 Oct 17;15(1):163. PMID: 37848946 -
Epilepsia
Targeted senotherapy improves electrographic and behavioral outcomes in a mouse model of temporal lobe epilepsy. [Abstract]2026 Apr 13. PMID: 41972901 -
Mol Immunol
STK39 interacts with MAPK14 to facilitate cardiomyocyte pyroptosis in septic cardiomyopathy by inducing NLRP3 inflammasome. [Abstract]2026 Oct:198:121-136. PMID: 42600550
Solvente y solubilidad
In Vitro:
DMSO : ≥ 100 mg/mL (126.81 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (6.34 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2681 mL | 6.3403 mL | 12.6805 mL | 31.7013 mL |
| 5 mM | 0.2536 mL | 1.2681 mL | 2.5361 mL | 6.3403 mL | |
| 10 mM | 0.1268 mL | 0.6340 mL | 1.2681 mL | 3.1701 mL | |
| 15 mM | 0.0845 mL | 0.4227 mL | 0.8454 mL | 2.1134 mL | |
| 20 mM | 0.0634 mL | 0.3170 mL | 0.6340 mL | 1.5851 mL | |
| 25 mM | 0.0507 mL | 0.2536 mL | 0.5072 mL | 1.2681 mL | |
| 30 mM | 0.0423 mL | 0.2113 mL | 0.4227 mL | 1.0567 mL | |
| 40 mM | 0.0317 mL | 0.1585 mL | 0.3170 mL | 0.7925 mL | |
| 50 mM | 0.0254 mL | 0.1268 mL | 0.2536 mL | 0.6340 mL | |
| 60 mM | 0.0211 mL | 0.1057 mL | 0.2113 mL | 0.5284 mL | |
| 80 mM | 0.0159 mL | 0.0793 mL | 0.1585 mL | 0.3963 mL | |
| 100 mM | 0.0127 mL | 0.0634 mL | 0.1268 mL | 0.3170 mL |
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