Glycerol kinase, microorganism  (Synonyms: GyK)

Glycerol kinase, microorganism (GyK) acts as a NR4A1 inhibitor with enzymatic activity. It directly binds to and inhibits the transcription factor NR4A1, thereby negatively regulating hepatic gluconeogenesis and reducing blood glucose levels. Glycerol kinase, microorganism positively regulates UCP1 expression via partial dependence on the β-adrenergic receptor-cAMP-CREB pathway, promotes browning of white adipose tissue and thermogenesis, and further modulates intracellular fatty acid composition and energy metabolism. In diabetic mouse models, overexpression of Glycerol kinase effectively antagonizes NR4A1-induced hyperglycemia, demonstrating potential for improving glucose homeostasis. Glycerol kinase, microorganism can be used for studies on diabetes and obesity^[1][2].

Recombinant GST-tagged microorganism-sourced glycerol kinase (overnight at 4°C) physically interacts with the DNA-binding domain and ligand-binding domain of NR4A1 in a cell-free GST pull-down assay^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9030-66-4

Solid

White to off-white

[Glycerol kinase, microorganism]

2.7.1.30

>180 U/mg protein

One unit is defined as the amount of enzyme that convert 1 μmol of glycerol to glycerol-3-phosphate per min at pH 7.9 at 37°C.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Miao L, et al. Glycerol kinase interacts with nuclear receptor NR4A1 and regulates glucose metabolism in the liver. FASEB J. 2019;33(6):6736-6747.  [Content Brief]

  [2]. Iwase M, et al. Glycerol kinase stimulates uncoupling protein 1 expression by regulating fatty acid metabolism in beige adipocytes. J Biol Chem. 2020;295(20):7033-7045.  [Content Brief]