Gymnoside II
Gymnoside II is one of the main bioactive constituents isolated from Bletilla striata. Gymnoside II inhibits nano SiO2-induced A549 cell viability reduction, apoptosis, and ROS generation by activating Nrf2. Gymnoside II upregulates HO-1 and γ-GCSc, while downregulating the Bax/Bcl-2 ratio. Gymnoside II is applicable for research on nano SiO2-induced pulmonary injury.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- CAS No.: 899430-02-5
- Formule: C21H30O11
- Masse moléculaire:458.46
-
Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
- Protocole
- Pureté et documentation
- Références
-
Help & FAQs
Activité biologique
Description
IC50 & Target
[1]|
HO-1 |
Bcl-xL |
Bcl-2 |
In Vitro
Gymnoside II (1–50 µM; 24 h) alleviated the reduction in A549 cell viability induced by nano-level SiO2 and did not affect the proliferation of normal cells [1].
Gymnoside II (25 μM; pre-treatment for 2 h + co-culture for 24 h) inhibits nm SiO2-induced apoptosis in A549 cells by normalizing the expression of apoptotic proteins and reducing the percentage of apoptotic cells [1].
Gymnoside II (25 µM; pretreatment for 2 h followed by co-culture for 24 h) reduces nm SiO2-induced ROS generation in A549 cells[1].
Gymnoside II (25 µM; pretreatment for 2 h followed by coculture for 24 h) upregulates the expression of Nrf2, HO-1, and γ-GCSc and activates the Nrf2 signaling pathway in A549 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:A549 cells
-
Concentration:1, 10, 25, 50 μM (with or without nm SiO₂)
-
Incubation Time:24 h
-
Result:Elevated the decreased cell viability induced by nm SiO₂ to a relative normal level.
Did not affect normal A549 cell proliferation.
-
Cell Line:A549 cells
-
Concentration:25 μM (pretreated for 2 h, then co-cultured with nm SiO₂)
-
Incubation Time:24 h (co-culture with nm SiO₂)
-
Result:Reduced the apoptotic rate from 28.5% to 8.72% and reversed aberrant expression of pro-apoptotic (Bax, Cleaved-caspase 3) and anti-apoptotic (Bcl-2) proteins.
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Cell Cytotoxicity Assay
- Mammalian live/dead viability and cytotoxicity staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- ROS/oxidative-stress fluorescent staining
- Apoptosis Solutions
- CCK-8/WST-8 Cell Proliferation Assay
- MTT Cell Proliferation Assay
- Cell Viability Determination by MTT Colorimetric Assay
Chemical Information
-
CAS No. 899430-02-5
-
Masse moléculaire 458.46
-
Formule C21H30O11
-
SMILES
OC[C@H]([C@H]([C@@H]([C@H]1O)O)O)O[C@H]1OC2=CC=C(C=C2)COC(C[C@](C(O)=O)(O)CC(C)C)=O
-
Structure Classification
-
Initial Source
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Gymnoside II Related Classifications
HY-N16929 Related Classifications
Powered by Bioz