Hexadecyl-CoA
Based on 1 Customer Validation
Hexadecyl-CoA is a non-hydrolyzable thioether analog of Palmitoyl-CoA. Hexadecyl-CoA competitively inhibits the acyl-CoA thioesterase activity of HNF-4α with a Ki of 0.3 μM, and inhibits DGAT2 activity with an IC50 of approximately 2 μM. Hexadecyl-CoA also inhibits ATGL activity and serves as a reference inhibitor for DGAT2 LC/MS enzyme activity assays. Hexadecyl-CoA can be used in studies related to lipid metabolism, triglyceride synthesis and lipolysis.
For research use only. We do not sell to patients.
- CAS No.: 71425-89-3
- Formula: C37H68N7O16P3S
- Molecular Weight:991.96
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
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HNF-4α1 0.3 μM (Ki) |
DGAT2 2 μM (IC50) |
In Vitro
Hexadecyl-CoA (50 μM; 20 min) inhibits TG hydrolase activity of ATGL in COS-7 cell lysates co-expressing ATGL and CGI-58, reducing activity to ~20% of control at 50 μM[1].
Hexadecyl-CoA (1-2.0 μM) potently inhibits the acyl-CoA thioesterase activity of recombinant rat HNF-4α1-LBD (aa132-455) with an apparent Ki of 0.3 μM, via competitive binding that requires its CoA-thioether structure[2].
Hexadecyl-CoA (10 μM) binds specifically to the acyl-CoA site (ACS) of recombinant rat HNF-4α1-LBD (aa132-455) with a stoichiometry of 0.8 nmol per nmol protein, is excluded from the fatty acid pocket (FAP), and requires the intact E-F domain for high-affinity binding[2].
Hexadecyl-CoA (up to 100 μM) does not displace the FAP-bound fluorescent marker C12-Bodipy from human HNF-4α1 recombinant, demonstrating it is excluded from the fatty acid pocket[2].
Hexadecyl-CoA (0.001-100 μM; 60 min) potently inhibits DGAT2 activity in human DGAT2-overexpressing Sf9 insect cell membranes with an IC50 of approximately 2 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 71425-89-3
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Appearance Solid
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Molecular Weight 991.96
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Formula C37H68N7O16P3S
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Color White to off-white
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SMILES
O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(OP(O)(OCC(C)([C@H](C(NCCC(NCCSCCCCCCCCCCCCCCCC)=O)=O)O)C)=O)=O)O[C@H]1N2C3=NC=NC(N)=C3N=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro:
H2O : ≥ 50 mg/mL (50.41 mM)
* "≥" means soluble, but saturation unknown.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 1.0081 mL | 5.0405 mL | 10.0811 mL | 25.2026 mL |
| 5 mM | 0.2016 mL | 1.0081 mL | 2.0162 mL | 5.0405 mL | |
| 10 mM | 0.1008 mL | 0.5041 mL | 1.0081 mL | 2.5203 mL | |
| 15 mM | 0.0672 mL | 0.3360 mL | 0.6721 mL | 1.6802 mL | |
| 20 mM | 0.0504 mL | 0.2520 mL | 0.5041 mL | 1.2601 mL | |
| 25 mM | 0.0403 mL | 0.2016 mL | 0.4032 mL | 1.0081 mL | |
| 30 mM | 0.0336 mL | 0.1680 mL | 0.3360 mL | 0.8401 mL | |
| 40 mM | 0.0252 mL | 0.1260 mL | 0.2520 mL | 0.6301 mL | |
| 50 mM | 0.0202 mL | 0.1008 mL | 0.2016 mL | 0.5041 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Hexadecyl-CoA Related Classifications
HY-E70277 Related Classifications
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