HPPE (Synonyms: Bach1-IN-1)

Name: HPPE
Brand: MedChemExpress
SKU: HY-153040
Rating: 5.0 (3 reviews)

Cat. No.: HY-153040 Purity: 99.71%: Data Sheet
COA

SDS
Handling Instructions
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HPPE (compound 236) is an orally active, potential Bach1 inhibitor. Bach1 is a transcription factor of the cap'n'collar type alkaline region leucine zipper factor family (CNC-bZip) that regulates mitochondrial metabolism and reduces glucose utilization. HPPE can be used for research in psoriasis, multiple sclerosis, and COPD.

For research use only. We do not sell to patients.

CAS No. : 1325721-55-8

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE HPPE

: HPPE purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Jul 15;44(1):206. [Abstract]; Western blot analysis was used to detect the protein and phosphorylation levels of PRC1 in cells after treatment. H1299 cells were subcutaneously injected into nude mice to construct xenografts, followed by intratumoral injections (10 mg/kg: Bobcat339, HPPE, or PLK1-IN-10) starting from day 7.

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Description

IC₅₀ & Target

Bach1^[1]

In Vitro

HPPE (0-20 μM, 18 h) is competitively inhibited by the overexpressed WT Bach1 in HepG2 cells^[3].
HPPE (3 μM, 1 h)-induced Bach1 derepression requires nuclear export of Bach1 and exhibited Nrf2 stabilization activity in M17-5 neuroblastoma cells^[3].
HPPE (10 μM, 8 h) activates NRF2 driven transcription independently of BACH1 in HEK293T cells^[4].
HPPE (10 μM, 1 h) liberates cellular zinc and increases cellular ROS levels in HEK293T cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	M17-5 neuroblastoma cells
Concentration:	3 μM
Incubation Time:	1 h
Result:	Significantly up-regulated Hmox1 (a Bach1 target) protein levels and showed accumulation of Nrf2 protein in the cytosol and especially in the nucleus in M17-5 neuroblastoma cells.

Western Blot Analysis^[4]

Cell Line:	HEK293T cells
Concentration:	10 μM
Incubation Time:	8 h
Result:	Robustly stabilized NRF2 protein levels within 8 hours of treatment in HEK293T cells.

In Vivo

HPPE (5 and 10 mg/kg, oral gavage, twice a day for 6 days) ameliorates MPTP-induced dopaminergic neurodegeneration and associated oxidative stress and neuroinflammation in the acute MPTP mouse model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	The acute MPTP mouse model^[2]
Dosage:	5 and 10 mg/kg
Administration:	oral gavage, twice a day for 6 days
Result:	Significantly protected SNpc dopaminergic neurons against MPTP neurotoxicity.

Molecular Weight

479.48

Formula

C₂₁H₂₀F₃N₅O₃S

CAS No.

1325721-55-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC(N=C(N2C)NC3=NC4=C(S3)C=C(C(F)(F)F)C=C4)=C2C=C1)NCCOCCO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (208.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0856 mL	10.4280 mL	20.8559 mL
5 mM	0.4171 mL	2.0856 mL	4.1712 mL
10 mM	0.2086 mL	1.0428 mL	2.0856 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.71%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (245 KB)

Handling Instructions (2659 KB)

References

[1]. Attucks, et al. Bach 1 inhibitors in combination with Nrf2 activators and pharmaceutical compositions thereof. World Intellectual Property Organization, WO2016089648 A1 2016-06-09.

[2]. Zhang X, et al. Bach1: Function, Regulation, and Involvement in Disease. Oxid Med Cell Longev. 2018 Oct 2;2018:1347969. [Content Brief]

[3]. Ahuja M, et al. Bach1 derepression is neuroprotective in a mouse model of Parkinson's disease. Proc Natl Acad Sci U S A. 2021 Nov 9;118(45):e2111643118. [Content Brief]

[4]. Freeman R, et al. HPPE Activates NRF2 Signaling by Liberating Heavy Metal Stores. Chembiochem. 2024 Sep 6:e202400529. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0856 mL	10.4280 mL	20.8559 mL	52.1398 mL
	5 mM	0.4171 mL	2.0856 mL	4.1712 mL	10.4280 mL
	10 mM	0.2086 mL	1.0428 mL	2.0856 mL	5.2140 mL
	15 mM	0.1390 mL	0.6952 mL	1.3904 mL	3.4760 mL
	20 mM	0.1043 mL	0.5214 mL	1.0428 mL	2.6070 mL
	25 mM	0.0834 mL	0.4171 mL	0.8342 mL	2.0856 mL
	30 mM	0.0695 mL	0.3476 mL	0.6952 mL	1.7380 mL
	40 mM	0.0521 mL	0.2607 mL	0.5214 mL	1.3035 mL
	50 mM	0.0417 mL	0.2086 mL	0.4171 mL	1.0428 mL
	60 mM	0.0348 mL	0.1738 mL	0.3476 mL	0.8690 mL
	80 mM	0.0261 mL	0.1303 mL	0.2607 mL	0.6517 mL
	100 mM	0.0209 mL	0.1043 mL	0.2086 mL	0.5214 mL

HPPE Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HPPE1325721-55-8Bach1-IN-1Mitochondrial MetabolismBach1mitochondrial metabolismglucose utilizationpsoriasismultiple sclerosisCOPDInhibitorinhibitorinhibit

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HPPE (Synonyms: Bach1-IN-1)

Customer Review

HPPE Related Antibodies

3 Publications Citing Use of MCE HPPE

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References

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HPPE Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

HPPE Related Classifications

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