Art. -Nr.: HY-153040

HPPE

(Synonyms: Bach1-IN-1)

3 Cited Publications

Kundenbewertung

Based on 3 publication(s) in Google Scholar

HPPE (compound 236) is an orally active, potential Bach1 inhibitor. Bach1 is a transcription factor of the cap'n'collar type alkaline region leucine zipper factor family (CNC-bZip) that regulates mitochondrial metabolism and reduces glucose utilization. HPPE can be used for research in psoriasis, multiple sclerosis, and COPD.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.71%
CAS. Nr.: 1325721-55-8
Formel: C₂₁H₂₀F₃N₅O₃S
Molecular Weight:479.48

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

HPPE Chemical Structure

CAS. Nr.: 1325721-55-8

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Bach1^[1]

In Vitro

HPPE (0-20 μM, 18 h) is competitively inhibited by the overexpressed WT Bach1 in HepG2 cells^[3].
HPPE (3 μM, 1 h)-induced Bach1 derepression requires nuclear export of Bach1 and exhibited Nrf2 stabilization activity in M17-5 neuroblastoma cells^[3].
HPPE (10 μM, 8 h) activates NRF2 driven transcription independently of BACH1 in HEK293T cells^[4].
HPPE (10 μM, 1 h) liberates cellular zinc and increases cellular ROS levels in HEK293T cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

M17-5 neuroblastoma cells
Concentration:

3 μM
Incubation Time:

1 h
Result:

Significantly up-regulated Hmox1 (a Bach1 target) protein levels and showed accumulation of Nrf2 protein in the cytosol and especially in the nucleus in M17-5 neuroblastoma cells.

Western Blot Analysis^[4]

Cell Line:

HEK293T cells
Concentration:

10 μM
Incubation Time:

8 h
Result:

Robustly stabilized NRF2 protein levels within 8 hours of treatment in HEK293T cells.

In Vivo

HPPE (5 and 10 mg/kg, oral gavage, twice a day for 6 days) ameliorates MPTP-induced dopaminergic neurodegeneration and associated oxidative stress and neuroinflammation in the acute MPTP mouse model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

The acute MPTP mouse model^[2]
Dosage:

5 and 10 mg/kg
Administration:

oral gavage, twice a day for 6 days
Result:

Significantly protected SNpc dopaminergic neurons against MPTP neurotoxicity.

Chemical Information

CAS. Nr. 1325721-55-8
Appearance Solid
Molecular Weight 479.48
Formel C₂₁H₂₀F₃N₅O₃S
Color White to off-white
SMILES

O=C(C1=CC(N=C(N2C)NC3=NC4=C(S3)C=C(C(F)(F)F)C=C4)=C2C=C1)NCCOCCO
Synonyms

Bach1-IN-1
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (3)

All Cell Proliferation/Viability AssayIn Vivo Efficacy StudyBio/Physico-chemical AssayFlow CytometryWBELISAIHCHistological Imaging/Staining

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Journal Impact Factor

J Nanobiotechnology

Ferrocene-derived magnetic fiber-particles from diesel exhaust: enhanced pulmonary toxicity via Bach1-SAT1-polyamine depletion. [Abstract]

2025 Apr 29;23(1):324. PMID: 40301891

HPPE purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Apr 29;23(1):324. [Abstract]

Viability of 16HBE cells following 48 h exposure to FMP (20 µg/ml) with or without pretreatment with 0.6 µM HPPE for 2 h. A 1 mM hydrogen peroxide treatment for 4 h was used as a positive control. (N = 5).

HPPE purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Apr 29;23(1):324. [Abstract]

Quantification of intracellular Fe^{2 +} levels in 16HBE cells following 48 h exposure to FMP (20 µg/ml) with or without pretreatment with 0.6 µM HPPE for 2 h (N = 3).

HPPE purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Apr 29;23(1):324. [Abstract]

HPPE (0.6 µM, 4 h). Quantification of ROS levels in 16HBE cells. Cells were treated with 50 µg/ml ROSUP for 2 h as a positive control. (N = 5).

HPPE purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Apr 29;23(1):324. [Abstract]

Representative Western blot images showing key molecular proteins (Bach1, ALOX15, SLC7A11, SAT1, GPX4) in 16HBE cells following 48 h exposure to FMP (20 µg/ml) with or without pretreatment with 0.6 µM HPPE for 2 h.

HPPE purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Apr 29;23(1):324. [Abstract]

ELISA quantifications of spermine (SP) and spermidine (SPD) levels in 16HBE cells following 48 h exposure to FMP (20 µg/ml) with or without pretreatment with 0.6 µM HPPE for 2 h (N = 5).

HPPE purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Apr 29;23(1):324. [Abstract]

Representative IHC images showing Bach1 and SAT1 protein expression in chicken lung tissues exposed to 0.6 mg/kg FMP, with or without pretreatment of 4 mg/kg HPPE (air cell injection, single dose). Each image was captured under a 400 × optical microscope, with the scale bar in the lower-right corner representing 100 μm. The lower-left corner of the image indicates a 200 × magnification of the field of view, with the scale bar representing 100 μm.

HPPE purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Apr 29;23(1):324. [Abstract]

Representative pathological pictures of chicken lung tissue sections from animals exposed to 0.6 mg/kg FMP, with or without pretreatment of 4 mg/kg HPPE (air cell injection, single dose), Yellow arrows represent positive areas.
J Exp Clin Cancer Res

CCNY-mediated phosphorylation and TET2-BACH1-driven DNA demethylation activate PRC1 to augment NSCLC progression. [Abstract]

2025 Jul 15;44(1):206. PMID: 40665337

HPPE purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Jul 15;44(1):206. [Abstract]

H1299 cells were subcutaneously injected into nude mice to construct xenografts, followed by intratumoral injections (10 mg/kg; Bobcat339, HPPE, or PLK1-IN-10) starting from day 7. Volume of xenograft tumors by week.
Br J Pharmacol

The neuronal ALAS2/5-ala axis mitigates chemotherapy-induced neurotoxicity via the BACH1/NRF2 pathway. [Abstract]

2026 Jun;183(11):2695-2719. PMID: 41652971

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (208.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0856 mL

10.4280 mL

20.8559 mL

5 mM

0.4171 mL

2.0856 mL

4.1712 mL

10 mM

0.2086 mL

1.0428 mL

2.0856 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Attucks, et al. Bach 1 inhibitors in combination with Nrf2 activators and pharmaceutical compositions thereof. World Intellectual Property Organization, WO2016089648 A1 2016-06-09.

[2]. Zhang X, et al. Bach1: Function, Regulation, and Involvement in Disease. Oxid Med Cell Longev. 2018 Oct 2;2018:1347969. [Content Brief]

[3]. Ahuja M, et al. Bach1 derepression is neuroprotective in a mouse model of Parkinson's disease. Proc Natl Acad Sci U S A. 2021 Nov 9;118(45):e2111643118. [Content Brief]

[4]. Freeman R, et al. HPPE Activates NRF2 Signaling by Liberating Heavy Metal Stores. Chembiochem. 2024 Sep 6:e202400529. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0856 mL	10.4280 mL	20.8559 mL	52.1398 mL
	5 mM	0.4171 mL	2.0856 mL	4.1712 mL	10.4280 mL
	10 mM	0.2086 mL	1.0428 mL	2.0856 mL	5.2140 mL
	15 mM	0.1390 mL	0.6952 mL	1.3904 mL	3.4760 mL
	20 mM	0.1043 mL	0.5214 mL	1.0428 mL	2.6070 mL
	25 mM	0.0834 mL	0.4171 mL	0.8342 mL	2.0856 mL
	30 mM	0.0695 mL	0.3476 mL	0.6952 mL	1.7380 mL
	40 mM	0.0521 mL	0.2607 mL	0.5214 mL	1.3035 mL
	50 mM	0.0417 mL	0.2086 mL	0.4171 mL	1.0428 mL
	60 mM	0.0348 mL	0.1738 mL	0.3476 mL	0.8690 mL
	80 mM	0.0261 mL	0.1303 mL	0.2607 mL	0.6517 mL
	100 mM	0.0209 mL	0.1043 mL	0.2086 mL	0.5214 mL

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HPPE

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Reprogramming Compound Library

Oxygen Sensing Compound Library

Orally Active Compound Library

Glutamine Metabolism Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Mitochondria-Targeted Compound Library

Glucose Metabolism Compound Library

Cuproptosis Compound Library

Cancer Stem Cells Compound Library

Mitochondrial Protection Compound Library

Metabolic Enzyme Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) HPPE

Biologische Aktivität

Chemical Information

Publications (3)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

HPPE

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Reprogramming Compound Library

Oxygen Sensing Compound Library

Orally Active Compound Library

Glutamine Metabolism Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Mitochondria-Targeted Compound Library

Glucose Metabolism Compound Library

Cuproptosis Compound Library

Cancer Stem Cells Compound Library

Mitochondrial Protection Compound Library

Metabolic Enzyme Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) HPPE

Biologische Aktivität

Chemical Information

Publications (3)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

HPPE Related Classifications

HY-153040 Related Classifications