Hyaluronidase 2  (Synonyms: EC:3.2.1.35; HYAL2)

Hyaluronidase 2 (EC:3.2.1.35; HYAL2) is a hyaluronidase. Hyaluronidase 2 cleaves high-molecular-weight hyaluronan into intermediate, ~20 kDa, and tetrasaccharide fragments. Hyaluronidase 2 directly interacts with CD44 and ERM proteins, reduces CD44 hyaluronan binding capacity and ERM activation. Hyaluronidase 2 can be used for the research of gliomas, inflammatory bowel disease, congenital heart defects, heart failure^{[1][2][3][4][5]}.

Hyaluronidase 2 directly associates with CD44 in BB16 rat fibroblastic cells, an interaction that requires an acidic pericellular microenvironment but is unaffected by exogenous hyaluronan (HA)^[1].
Hyaluronidase 2 (48 h) induces purified human monocytes to produce proinflammatory cytokines IL-6 and IL-8, with this activity dependent on intact HA structure^[2].
Hyaluronidase 2 (18 h) mediates degradation of immobilized purified HA exclusively under acidic conditions (optimum pH 3.5)^[3].
Hyaluronidase 2 (6 weeks) negatively regulates RON receptor tyrosine kinase function in BEAS-2B immortalized human bronchial epithelial cells by maintaining RON in an inactive state through physical association^[5].
Hyaluronidase 2 (2 days) reduces ligand-independent RON tyrosine phosphorylation and MAPK activation induced by JSRV Env in RE7 Madin-Darby canine kidney cells stably expressing human RON, by maintaining association with RON and preventing Env-mediated disruption of this complex^[5].
Hyaluronidase 2 (48 h) physically associates with RON in HEK293 human embryonic kidney cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hyaluronidase 2 deficiency in mice increases plasma hyaluronan levels 10-fold and induces skeletal and hematological anomalies^[1].
Hyaluronidase 2 loss significantly increases susceptibility to DSS (HY-116282)-induced colitis in mice, as evidenced by greater weight loss, higher DAI scores, and increased inflammatory cell infiltration^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

[Hyaluronidase 2]

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Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Duterme C, et al. Two novel functions of hyaluronidase-2 (Hyal2) are formation of the glycocalyx and control of CD44-ERM interactions. J Biol Chem. 2009;284(48):33495-33508.  [Content Brief]

  [2]. de la Motte C, et al. Platelet-derived hyaluronidase 2 cleaves hyaluronan into fragments that trigger monocyte-mediated production of proinflammatory cytokines. Am J Pathol. 2009;174(6):2254-2264.  [Content Brief]

  [3]. Albeiroti S, et al. Platelet hyaluronidase-2: an enzyme that translocates to the surface upon activation to function in extracellular matrix degradation. Blood. 2015;125(9):1460-1469.  [Content Brief]

  [4]. Petrey AC, et al. Platelet hyaluronidase-2 regulates the early stages of inflammatory disease in colitis. Blood. 2019;134(9):765-775.  [Content Brief]

  [5]. Danilkovitch-Miagkova A, et al. Hyaluronidase 2 negatively regulates RON receptor tyrosine kinase and mediates transformation of epithelial cells by jaagsiekte sheep retrovirus. Proc Natl Acad Sci U S A. 2003;100(8):4580-4585.  [Content Brief]