Hypophyllanthin
Based on 1 Customer Validation
Hypophyllanthin is a major lignan in Phyllanthus spp, with strong anti-inflammatory activity. Hypophyllanthin directly inhibits P-glycoprotein (P-gp) activity and did not interfere with multidrug resistance protein 2 (MRP2) activity.
For research use only. We do not sell to patients.
- Purity: 98.84%
- CAS No.: 33676-00-5
- Formula: C24H30O7
- Molecular Weight:430.49
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Immunology/Inflammation Compound Library
HY-L007
-
Membrane Transporter/Ion Channel Compound Library
HY-L011
-
Natural Product Library
HY-L021
-
Anti-Cancer Compound Library
HY-L025
-
Pyroptosis Compound Library
HY-L059
-
Anti-Cancer Natural Product Library
HY-L107
-
Plant-Sourced Natural Product Library
HY-L115
-
Human Metabolite Library
HY-L123
-
Cancer Stem Cells Compound Library
HY-L135
-
Anti-Drug-Resistant Compound Library
HY-L169
-
Anti-Ovarian Cancer Compound Library
HY-L173
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Natural Product Library Plus
HY-L021P
-
Natural Product and Natural Product-Like Compound Library
HY-L021M
-
Bioactive Compound Library
HY-L001
-
RO5 Drug-like Natural Product Library
HY-L200
-
High-Throughput Bioactive Compound Library
HY-L205
-
High-Throughput Natural Product Library
HY-L206
-
Mass Spectrometry Human Metabolite Library
HY-L215
-
Natural Product Diversity Scaffold Library
HY-L245
-
Mass Spectrometry Natural Product Library
HY-L262
Biological Activity
P-gp[2]
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A10 | IC50 |
580 μM
Compound: 2
|
Displacement of [125I]endothelin-1 from ETA receptor in rat A10 cells by scintillation counting
Displacement of [125I]endothelin-1 from ETA receptor in rat A10 cells by scintillation counting
|
10.1021/np50124a006 |
| A-431 | ED50 |
>20 μg/mL
Compound: 2
|
Cytotoxicity against human A431 cells after 2 to 4 days by SRB assay
Cytotoxicity against human A431 cells after 2 to 4 days by SRB assay
|
[PMID: 8385184] |
| Col2 | ED50 |
>20 μg/mL
Compound: 2
|
Cytotoxicity against human Col2 cells after 2 to 4 days by SRB assay
Cytotoxicity against human Col2 cells after 2 to 4 days by SRB assay
|
[PMID: 8385184] |
| KB-V1 | ED50 |
>20 μg/mL
Compound: 2
|
Cytotoxicity against multidrug-resistant human KBV1 cells after 2 to 4 days by SRB assay in absence of vinblastine
Cytotoxicity against multidrug-resistant human KBV1 cells after 2 to 4 days by SRB assay in absence of vinblastine
|
[PMID: 8385184] |
| KB-V1 | ED50 |
3.8 μg/mL
Compound: 2
|
Cytotoxicity against multidrug-resistant human KBV1 cells after 2 to 4 days by SRB assay in presence of vinblastine
Cytotoxicity against multidrug-resistant human KBV1 cells after 2 to 4 days by SRB assay in presence of vinblastine
|
[PMID: 8385184] |
| LNCaP | ED50 |
>20 μg/mL
Compound: 2
|
Cytotoxicity against human LNCAP cells after 2 to 4 days by SRB assay
Cytotoxicity against human LNCAP cells after 2 to 4 days by SRB assay
|
[PMID: 8385184] |
| P388 | ED50 |
>20 μg/mL
Compound: 2
|
Cytotoxicity against mouse P388 cells after 2 to 4 days by SRB assay
Cytotoxicity against mouse P388 cells after 2 to 4 days by SRB assay
|
[PMID: 8385184] |
| SK-MEL-2 | ED50 |
>20 μg/mL
Compound: 2
|
Cytotoxicity against human SK-MEL-2 cells after 2 to 4 days by SRB assay
Cytotoxicity against human SK-MEL-2 cells after 2 to 4 days by SRB assay
|
[PMID: 8385184] |
| ZR-75-1 | ED50 |
>20 μg/mL
Compound: 2
|
Cytotoxicity against human ZR-75-1 cells after 2 to 4 days by SRB assay
Cytotoxicity against human ZR-75-1 cells after 2 to 4 days by SRB assay
|
[PMID: 8385184] |
Chemical Information
-
CAS No. 33676-00-5
-
Appearance Solid
-
Molecular Weight 430.49
-
Formula C24H30O7
-
Color White to off-white
-
SMILES
COC[C@@H]1[C@@H](COC)CC2=CC(OC)=C(OCO3)C3=C2[C@H]1C4=CC=C(OC)C(OC)=C4
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 33.33 mg/mL (77.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.81 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (279 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
[1]. Harikrishnan H, et al. Anti-Inflammatory Effects of Hypophyllanthin and Niranthin Through Downregulation of NF-κB/MAPKs/PI3K-Akt Signaling Pathways. Inflammation. 2018 Jun;41(3):984-995. [Content Brief]
[2]. Sukhaphirom N, et al. Phyllanthin and hypophyllanthin inhibit function of P-gp but not MRP2 in Caco-2 cells. J Pharm Pharmacol. 2013 Feb;65(2):292-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3229 mL | 11.6147 mL | 23.2293 mL | 58.0734 mL |
| 5 mM | 0.4646 mL | 2.3229 mL | 4.6459 mL | 11.6147 mL | |
| 10 mM | 0.2323 mL | 1.1615 mL | 2.3229 mL | 5.8073 mL | |
| 15 mM | 0.1549 mL | 0.7743 mL | 1.5486 mL | 3.8716 mL | |
| 20 mM | 0.1161 mL | 0.5807 mL | 1.1615 mL | 2.9037 mL | |
| 25 mM | 0.0929 mL | 0.4646 mL | 0.9292 mL | 2.3229 mL | |
| 30 mM | 0.0774 mL | 0.3872 mL | 0.7743 mL | 1.9358 mL | |
| 40 mM | 0.0581 mL | 0.2904 mL | 0.5807 mL | 1.4518 mL | |
| 50 mM | 0.0465 mL | 0.2323 mL | 0.4646 mL | 1.1615 mL | |
| 60 mM | 0.0387 mL | 0.1936 mL | 0.3872 mL | 0.9679 mL |
Powered by Bioz