IOX4
Based on 7 publication(s) in Google Scholar
IOX4 is a selective HIF prolyl-hydroxylase 2 (PHD2) inhibitor with an IC50 value of 1.6 nM, induces HIFα in cells and in wildtype mice with marked induction in the brain tissue. IOX4 competes with and displaces 2-oxoglutarate (2OG) at the active site of PHD2.
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 1154097-71-8
- Formula: C15H16N6O3
- Molecular Weight:328.33
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) IOX4
More- Phytomedicine. 2025 Mar:138:156392. [Abstract]
- J Pharm Anal. 2026 Jun;16(6):101494.
- Cell Rep. 2023 Sep 19;42(10):113158. [Abstract]
- Drug Test Anal. 2022 Jul;14(7):1244-1254. [Abstract]
- Drug Test Anal. 2022 Feb;14(2):233-251. [Abstract]
- Dev Comp Immunol. 2023 Mar:140:104598. [Abstract]
- bioRxiv. 2023 Jun 9:2023.06.07.544110. [Abstract]
Customer Validation & Images
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WB
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Histological Imaging/Staining
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Bio/Physico-chemical Assay
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In Vivo Efficacy Study
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IF
Biological Activity
IC50: 1.6 nM (PHD2)[1]
IOX4 (1-100 μM; 5 hours) determines the EC50s for HIF1α induction are 114 μM, 86 μM and 49.5 μM in MCF-7, Hep3B and U2OS, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1154097-71-8
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Appearance Solid
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Molecular Weight 328.33
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Formula C15H16N6O3
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Color Light yellow to yellow
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SMILES
O=C(C1=CC=C(N2NC=C(N3N=NC=C3)C2=O)N=C1)OC(C)(C)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (7)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Polydatin mitigates thrombosis by inhibiting PHD2-induced proline hydroxylation on collagen, reducing platelet adhesion. [Abstract]2025 Mar:138:156392. PMID: 39826283 -
IOX4 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2026 Jun;16(6):101494.
HIF-1α expression post IOX4 (10 mg/kg; i.p.; once a week for six weeks) and DIO treatment in vivo (n = 3): representative Western blot (WB) images.
IOX4 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2026 Jun;16(6):101494.
Representative images (up) and hematoxylin and eosin (H&E) staining (down) of footpads after IOX4 (10 mg/kg; i.p.; once a week for six weeks) and DIO treatment (n = 3).
IOX4 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2026 Jun;16(6):101494.
Serum uric acid (S-UA) and urinary uric acid (U-UA) levels post IOX4 (10 mg/kg; i.p.; once a week for six weeks) and DIO treatment (n = 6–10).
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Cell Rep
2023 Sep 19;42(10):113158. PMID: 37733588
IOX4 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Sep 19;42(10):113158. [Abstract]
IOX4 (30 mg/kg; i.p.; once daily) activated by HIF1 reduced TUNEL-positive rates in 3-week-old C57BL/6J female mice.
IOX4 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Sep 19;42(10):113158. [Abstract]
TUNEL and immunofluorescence staining of p-AMPK under control, PMSG-, and IOX4 (30 mg/kg; i.p.; once daily)-treated conditions; scale bars, 100 mm.
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Drug Test Anal
Long-term monitoring of IOX4 in horse hair and its longitudinal distribution with segmental analysis using liquid chromatography/electrospray ionization Q Exactive high-resolution mass spectrometry for the purpose of doping control. [Abstract]2022 Jul;14(7):1244-1254. PMID: 35195358 -
Drug Test Anal
Comprehensive metabolic study of IOX4 in equine urine and plasma using liquid chromatography/electrospray ionization Q Exactive high-resolution mass spectrometer for the purpose of doping control. [Abstract]2022 Feb;14(2):233-251. PMID: 34612014 -
Dev Comp Immunol
The involvement of hypoxia inducible factor-1α on the proportion of three types of haemocytes in Chinese mitten crab under hypoxia stress. [Abstract]2023 Mar:140:104598. PMID: 36511346 -
bioRxiv
2023 Jun 9:2023.06.07.544110. PMID: 37333192
Solvent & Solubility
DMSO : 50 mg/mL (152.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (7.61 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.0457 mL | 15.2286 mL | 30.4572 mL | 76.1429 mL |
| 5 mM | 0.6091 mL | 3.0457 mL | 6.0914 mL | 15.2286 mL | |
| 10 mM | 0.3046 mL | 1.5229 mL | 3.0457 mL | 7.6143 mL | |
| 15 mM | 0.2030 mL | 1.0152 mL | 2.0305 mL | 5.0762 mL | |
| 20 mM | 0.1523 mL | 0.7614 mL | 1.5229 mL | 3.8071 mL | |
| 25 mM | 0.1218 mL | 0.6091 mL | 1.2183 mL | 3.0457 mL | |
| 30 mM | 0.1015 mL | 0.5076 mL | 1.0152 mL | 2.5381 mL | |
| 40 mM | 0.0761 mL | 0.3807 mL | 0.7614 mL | 1.9036 mL | |
| 50 mM | 0.0609 mL | 0.3046 mL | 0.6091 mL | 1.5229 mL | |
| 60 mM | 0.0508 mL | 0.2538 mL | 0.5076 mL | 1.2690 mL | |
| 80 mM | 0.0381 mL | 0.1904 mL | 0.3807 mL | 0.9518 mL | |
| 100 mM | 0.0305 mL | 0.1523 mL | 0.3046 mL | 0.7614 mL |
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