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Cat. No.: HY-12352

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HJC0416

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

HJC0416 is a potent and orally active STAT3 inhibitor. HJC0416 shows antiprolifeative activity and induces Apoptosis. HJC0416 decreases the expression of p-STAT3 (Tyr-705), Cyclin D1 and increases the expression of cleaved caspase-3 protein. HJC0416 shows anti-tumor activity.

For research use only. We do not sell to patients.

CAS No.: 1617518-22-5
Formula: C₁₈H₁₇ClN₂O₄S
Molecular Weight:392.86

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (276 KB)

HJC0416 Chemical Structure

CAS No.: 1617518-22-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

STAT3

All STAT Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
ASPC1	IC₅₀	0.04 μM Compound: 12, HJC0416	Antiproliferative activity against human AsPC1 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay Antiproliferative activity against human AsPC1 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay	[PMID: 24904966]
MCF7	IC₅₀	1.76 μM Compound: 12, HJC0416	Antiproliferative activity against ER-positive human MCF7 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay Antiproliferative activity against ER-positive human MCF7 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay	[PMID: 24904966]
MDA-MB-231	IC₅₀	1.97 μM Compound: 12, HJC0416	Antiproliferative activity against ER-negative and triple-negative human MDA-MB-231 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay Antiproliferative activity against ER-negative and triple-negative human MDA-MB-231 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay	[PMID: 24904966]
PANC-1	IC₅₀	1.88 μM Compound: 12, HJC0416	Antiproliferative activity against human PANC1 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay Antiproliferative activity against human PANC1 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay	[PMID: 24904966]

In Vitro

HJC0416 (0-10 µM; 48 h) shows antiprolifeative activity and induces apoptosis in MDA-MB-231 cells^[1].
HJC0416 (0-10 µM; 12 h) decreases the expression of p-STAT3 (Tyr-705), Cyclin D1 and increases the expression of cleaved caspase-3 protein^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

MCF-7, MDA-MB-231, AsPC1, Panc-1 cells
Concentration:

0-100 µM
Incubation Time:

72 h
Result:

Showed antiproliferative activity with IC₅₀s 1.76, 1.97, 0.04, 1.88 µM for MCF-7, MDA-MB-231, AsPC1, Panc-1 cells, respectively.

Apoptosis Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

1, 5, 10 µM
Incubation Time:

48 h
Result:

Induced cell apoptosis.

Western Blot Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

0, 1, 5, 10 µM
Incubation Time:

12 h
Result:

Suppressed the expression of phosphorylated STAT3 at Tyr-705, Cyclin D1, increased the expression of cleaved caspase-3.

In Vivo

HJC0416 (10 mg/kg for i.p.; 100 mg/kg for p.o.; daily for 7 days) reduces the tumor growth with no significant body weight loss in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

6 weeks, female nude mice (MDA-MB-231 cells)^[1]
Dosage:

10 mg/kg for i.p.; 100 mg/kg for p.o.
Administration:

I.p. or p.o.; daily for 7 days
Result:

Decreased the tumor volume for 67% as compared to the control mice for i.p.; the growth of xenograft tumors in mice was also significantly reduced at a dose of 100 mg/kg by 46%.

Chemical Information

CAS No. 1617518-22-5
Molecular Weight 392.86
Formula C₁₈H₁₇ClN₂O₄S
SMILES

O=C(NC1=CC=C(C=CS2(=O)=O)C2=C1)C3=CC(Cl)=CC=C3OCCCN
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (1)

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Journal Impact Factor

Cell Mol Gastroenterol Hepatol

Siglec-H-/- Plasmacytoid Dendritic Cells Protects Against Acute Liver Injury by Suppressing IFN-γ/Th1 Response and Promoting IL-21+ CD4 T Cells. [Abstract]

2024 Jun 5:101367. PMID: 38849082

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chen H, et al. Discovery of potent anticancer agent HJC0416, an orally bioavailable small molecule inhibitor of signal transducer and activator of transcription 3 (STAT3). Eur J Med Chem. 2014 Jul 23;82:195-203. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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