AP21998
Based on 1 Customer Validation
AP21998 is a non-immunosuppressive derivative of Rapamycin (HY-10219) and an inducer of exocytosis. By binding to mutant FKBP (F36M), AP21998 dissociates fusion protein aggregates in the endoplasmic reticulum into monomers, which then enter the Golgi apparatus to be cleaved by furin protease, ultimately releasing the polypeptide. AP21998 can be used in cancer research.
For research use only. We do not sell to patients.
- Purity: 99.40%
- CAS No.: 292606-90-7
- Formula: C43H59N3O10
- Molecular Weight:777.94
-
Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
AP21998 (1.5 μM; 2 h) exhibits activity to induce the expression and secretion of GFP and GDNF in D407 cells that are co-transduced with TEV and GFP secretion vectors, as well as those co-transduced with TEV and GDNF secretion vectors[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:D407 cells (lentiviral vector-cotransduced)
-
Concentration:1.5 μM
-
Incubation Time:2 h
-
Result:Increased GDNF secretion to levels approximately 20% higher than in cultures treated with AP21967 alone.
Produced the maximum measurable GDNF secretion.
Chemical Information
-
CAS No. 292606-90-7
-
Appearance Oil
-
Molecular Weight 777.94
-
Formula C43H59N3O10
-
Color Off-white to light yellow
-
SMILES
O=C(N1[C@@H](CCCC1)C(O[C@@H](C2=CC(OCC(NCCCN(C)C)=O)=CC=C2)CCC3=CC(OC)=C(C=C3)OC)=O)[C@H](C4=CC(OC)=C(C(OC)=C4)OC)CC
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (128.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.21 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.21 mM); Suspended solution
This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2854 mL | 6.4272 mL | 12.8545 mL | 32.1362 mL |
| 5 mM | 0.2571 mL | 1.2854 mL | 2.5709 mL | 6.4272 mL | |
| 10 mM | 0.1285 mL | 0.6427 mL | 1.2854 mL | 3.2136 mL | |
| 15 mM | 0.0857 mL | 0.4285 mL | 0.8570 mL | 2.1424 mL | |
| 20 mM | 0.0643 mL | 0.3214 mL | 0.6427 mL | 1.6068 mL | |
| 25 mM | 0.0514 mL | 0.2571 mL | 0.5142 mL | 1.2854 mL | |
| 30 mM | 0.0428 mL | 0.2142 mL | 0.4285 mL | 1.0712 mL | |
| 40 mM | 0.0321 mL | 0.1607 mL | 0.3214 mL | 0.8034 mL | |
| 50 mM | 0.0257 mL | 0.1285 mL | 0.2571 mL | 0.6427 mL | |
| 60 mM | 0.0214 mL | 0.1071 mL | 0.2142 mL | 0.5356 mL | |
| 80 mM | 0.0161 mL | 0.0803 mL | 0.1607 mL | 0.4017 mL | |
| 100 mM | 0.0129 mL | 0.0643 mL | 0.1285 mL | 0.3214 mL |
AP21998 Related Classifications
HY-179354 Related Classifications
Powered by Bioz