L-メチオニン
Based on 12 publication(s) in Google Scholar
L-Methionine is an L-isomer of orally active Methionine, an essential amino acid. Methionine is a strong liver antidote that acts as a liver protector. L-Methionine can inhibit cell proliferation and induce cell apoptosis. L-Methionine has antitumor and antioxidant activity.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.98%
- CAS 番号: 63-68-3
- 分子式: C5H11NO2S
- 分子量:149.22
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
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Help & FAQs
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MedChemExpress（MCE）の使用を引用している文献 L-Methionine
More- Nature. 2025 Jul;643(8070):192-200. [Abstract]
- Nat Genet. 2026 Jul 8.
- Nat Commun. 2025 Jan 31;16(1):1233. [Abstract]
- Adv Sci (Weinh). 2026 Mar 18:e22690. [Abstract]
- Adv Sci (Weinh). 2025 May;12(19):e2501623. [Abstract]
- Antioxidants (Basel). 2026 Sep 11;15(9):1162.
- Emerg Microbes Infect. 2025 Dec;14(1):2447620. [Abstract]
- Molecules. 2025 Mar 9;30(6):1224. [Abstract]
- Sci Rep. 2026 Jan 14;16(1):5201. [Abstract]
- Neoplasia. 2025 Jun:64:101160. [Abstract]
- J Chromatogr A. 2025 Aug 16:1755:466036. [Abstract]
- bioRxiv. 2024 August 18.
Customer Validation & Images
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WB
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ELISA
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IHC
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In Vivo Efficacy Study
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Flow Cytometry
Endogenous Metabolite アイソフォーム固有の製品をすべて表示
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生物活性
製品説明
IC50 & Target
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Microbial Metabolite |
Human Endogenous Metabolite |
体外実験
L-Methionine (5 mg/mL, 7 or 3 days) can inhibit the proliferation of BXPC-3 (mutant p53) and HPAC (wild-type p53) pancreatic cancer cells, interfere with cell cycle, and induce apoptosis of BXPC-3 cells[1].
L-Methionine (0.3-10 mM, 24 h) reduces the formation of free radicals in endothelial cells by inducing heme oxidase-1 and ferritin[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BXPC-3, HPAC
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Concentration:5 mg/mL
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Incubation Time:7 or 3 days
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Result:Reduced growth of BXPC-3 cells by 31 and 32%, respectively.
Reduced growth of HPAC cells by 35% and 18%, respectively.
Reduced S to G2 transition in BXPC-3 cells and intervened in both G1–S and S–G2 transitions in HPAC cells.
Increased late cell apoptosis at a percentage of 76% in BXPC-3 cells.
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Cell Line:ECV304
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Concentration:0.3, 1, 3, 5, 10 mM
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Incubation Time:24 h
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Result:Increased the level of HO-1 protein up to 9.5-fold and the HO-1 activity.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Wistar rats (body weight 70-80 g)[3]
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Dosage:215,268.8, 322.5, 430 mg/kg
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Administration:p.o.
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Result:Reduced the hepatic ROS levels and the hepatic MDA contents.
Increased the hepatic T-AOC, CAT and T-SOD.
Increased the hepatic activity of GCL, GR, GST and GPx.
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Apoptosis Solutions
- MTT Cell Proliferation Assay
化学情報
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CAS 番号 63-68-3
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性状 Solid
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分子量 149.22
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分子式 C5H11NO2S
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Color White to off-white
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SMILES
N[C@@H](CCSC)C(O)=O
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別名
L-Methionine
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Structure Classification
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (12)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
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Nat Commun
2025 Jan 31;16(1):1233. PMID: 39890804 -
Adv Sci (Weinh)
Cars2-Mediated Cysteine Catabolism Drives Brown Fat Development and Thermogenesis Through Persulfidating EBF2. [Abstract]2026 Mar 18:e22690. PMID: 41849685 -
Adv Sci (Weinh)
Methionine Metabolism Dictates PCSK9 Expression and Antitumor Potency of PD-1 Blockade in MSS Colorectal Cancer. [Abstract]2025 May;12(19):e2501623. PMID: 40125618
L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May;12(19):e2501623. [Abstract]
The expression and secretion of PCSK9 in human with methionine deprivation for 6 h and then methionine (0-50 μM) supplementation for 24h. Immunoblotting analysis of PCSK9 in human CRC cell lines (SW480, SW620, HCT116, and LoVo).
L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May;12(19):e2501623. [Abstract]
L-Methionine (0, 20, 50 μM). ELISA analysis of PCSK9 in culture supernatant of human CRC cell lines (SW480, SW620, HCT116, and LoVo).
L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May;12(19):e2501623. [Abstract]
Mice were housed on either a normal diet (ND) with 0.86% methionine or a dietary methionine restriction (DMR) diet with 0.12% methionine from the day after injection until the endpoints. Immunohistochemistry analysis of PCSK9 in tumors from MC38‐ (n = 9) and Colon 26‐ (n = 4 (ND), n = 8 (DMR)) bearing mice fed with normal diet and methionine restriction diet (left). The abundance of PCSK9 was assessed (right).
L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May;12(19):e2501623. [Abstract]
Mice were housed on either a normal diet (ND) with 0.86% methionine or a dietary methionine restriction (DMR) diet with 0.12% methionine from the day after injection until the endpoints. Tumor volume of PCSK9‐KD (n = 10) or PCSK9‐KO (n = 9) MC38‐bearing mice fed with normal diet and methionine restriction diet. EAA, essential amino acid; NEEA, non‐essential amino acid; CM, complete medium; ND, normal diet; DMR, dietary methionine restriction.
L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May;12(19):e2501623. [Abstract]
Mice were housed on either a normal diet (ND) with 0.86% methionine or a dietary methionine restriction (DMR) diet with 0.12% methionine from the day after injection until the endpoints. Flow cytometric analysis of TNFα and Granzyme B in intratumoral CD8+T cells from Colon 26‐bearing mice fed with a methionine restriction diet alone or in combination with anti‐PD‐1 therapy.
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Emerg Microbes Infect
SARS-CoV-2 and HCoV-OC43 regulate host m6A modification via activation of the mTORC1 signaling pathway to facilitate viral replication. [Abstract]2025 Dec;14(1):2447620. PMID: 39745173 -
Molecules
Seeking Correlation Among Porin Permeabilities and Minimum Inhibitory Concentrations Through Machine Learning: A Promising Route to the Essential Molecular Descriptors. [Abstract]2025 Mar 9;30(6):1224. PMID: 40142001 -
Sci Rep
Interaction between ELMO1 DNA methylation and Med31 promotes H. pylori-induced gastric cancer EMT and intestinal metaplasia via M2 polarization. [Abstract]2026 Jan 14;16(1):5201. PMID: 41535333 -
Neoplasia
L-methionine promotes CD8+ T cells killing hepatocellular carcinoma by inhibiting NR1I2/PCSK9 signaling. [Abstract]2025 Jun:64:101160. PMID: 40158232 -
J Chromatogr A
Quantification of five intracellular and extracellular methionine pathway intermediates using stable isotope dilution UHPLC-MS/MS. [Abstract]2025 Aug 16:1755:466036. PMID: 40398135 -
溶剤 & 溶解度
体外:
H2O : 10 mg/mL (67.02 mM; Need ultrasonic)
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 12.5 mg/mL (83.77 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
純度とドキュメンテーション
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データシート (276 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Benavides MA, et al. L-Methionine inhibits growth of human pancreatic cancer cells. Anticancer Drugs. 2014 Feb;25(2):200-3. [Content Brief]
[2]. Erdmann K, et al. L-methionine reduces oxidant stress in endothelial cells: role of heme oxygenase-1, ferritin, and nitric oxide. AAPS J. 2005 Aug 29;7(1):E195-200. [Content Brief]
[3]. Wang Z, et al. l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats. J Sci Food Agric. 2019 Aug 15;99(10):4849-4862. [Content Brief]
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 6.7015 mL | 33.5076 mL | 67.0151 mL | 167.5379 mL |
| 5 mM | 1.3403 mL | 6.7015 mL | 13.4030 mL | 33.5076 mL | |
| 10 mM | 0.6702 mL | 3.3508 mL | 6.7015 mL | 16.7538 mL | |
| 15 mM | 0.4468 mL | 2.2338 mL | 4.4677 mL | 11.1692 mL | |
| 20 mM | 0.3351 mL | 1.6754 mL | 3.3508 mL | 8.3769 mL | |
| 25 mM | 0.2681 mL | 1.3403 mL | 2.6806 mL | 6.7015 mL | |
| 30 mM | 0.2234 mL | 1.1169 mL | 2.2338 mL | 5.5846 mL | |
| 40 mM | 0.1675 mL | 0.8377 mL | 1.6754 mL | 4.1884 mL | |
| 50 mM | 0.1340 mL | 0.6702 mL | 1.3403 mL | 3.3508 mL | |
| 60 mM | 0.1117 mL | 0.5585 mL | 1.1169 mL | 2.7923 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Methionine 関連分類
HY-N0326 関連分類
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