Mafenide (Acetate)
Based on 1 Customer Validation
Mafenide Acetate is a potent sulfonamide antimicrobial agent. Mafenide Acetate exhibits antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. Mafenide Acetate also exhibits antifungal activity against filamentous fungi (e.g., Lichtheimia and Aspergillus flavus). Mafenide Acetate can be used in the research of skin grafts on burn wounds, post-traumatic invasive fungal infections, and bacterially contaminated wounds.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.29%
- CAS 番号: 13009-99-9
- 分子式: C9H14N2O4S
- 分子量:246.28
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保管条件:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
Mafenide Acetate (5%) maintains antimicrobial activity with a zone of inhibition >2 mm against Staphylococcus aureus and Pseudomonas aeruginosa in vitro[1].
Mafenide Acetate (2.5%-7.5%; 1-24 h) exhibits dose-dependent antifungal activity against filamentous fungi (e.g., Lichtheimia spp., Aspergillus flavus)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human keratinocytes (HEK-001; ATCC CRL-2404), human dermal fibroblasts (PromoCell), human osteoblasts (PromoCell)
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Concentration:2.5%, 5%, 7.5%
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Incubation Time:0.5 h, 1 h, 3 h, 24 h
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Result:Showed a viability range of 30%-78% (fibroblasts).
Showed a viability range of 31%-77% (keratinocytes).
Showed a viability range of 22%-81% (osteoblasts).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Wistar albino rats (mean weight 200 g) with Pseudomonas aeruginosa-contaminated full-thickness skin graft (FTSG) model (2.5×2.5 cm FTSG harvested from back, wound bed inoculated with 107 CFU/mL Pseudomonas aeruginosa in 0.6 mL buffer solution)[4]
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Dosage:2.5% Mafenide Acetate solution
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Administration:Topical application via soaked dressings (hydrofiber or gauze); group 3 (hydrofiber + MA): dressing changed every 2 days; group 4 (gauze + MA): dressing changed twice a day; total cycle of 14 days
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Result:Showed a mean graft survival rate of 50.1% in group 3 and 66.3% in group 4.
Showed detachment of the dermal-epidermal junction, collagen disorganization, decreased fibroblast number, and decreased capillary count.
化学情報
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CAS 番号 13009-99-9
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性状 Solid
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分子量 246.28
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分子式 C9H14N2O4S
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Color White to off-white
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SMILES
O=S(C1=CC=C(CN)C=C1)(N)=O.CC(O)=O
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別名
酢酸マフェニド
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
DMSO : 100 mg/mL (406.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 100 mg/mL (406.04 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.15 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 70 mg/mL (284.23 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
純度とドキュメンテーション
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取扱説明書 (2659 KB)
参考文献
[1]. Ashkan Afshari, et al. The Effective Duration of Antimicrobial Activity of Mafenide Acetate After Reconstitution. J Burn Care Res. 2018 Aug 17;39(5):736-738. [Content Brief]
[2]. Haynes, B.W., Jr., Mafenide acetate in burn treatment. N Engl J Med, 1971. 284(23): p. 1324. [Content Brief]
[3]. Barsoumian A, et al. In vitro toxicity and activity of Dakin's solution, mafenide acetate, and amphotericin B on filamentous fungi and human cells. J Orthop Trauma. 2013 Aug;27(8):428-36. [Content Brief]
[4]. Baver Acaban M, et al. The effects of topical mafenide acetate application on skin graft survival in bacterial contaminated wounds. Burns. 2024 Mar;50(2):433-443. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 4.0604 mL | 20.3021 mL | 40.6042 mL | 101.5105 mL |
| 5 mM | 0.8121 mL | 4.0604 mL | 8.1208 mL | 20.3021 mL | |
| 10 mM | 0.4060 mL | 2.0302 mL | 4.0604 mL | 10.1510 mL | |
| 15 mM | 0.2707 mL | 1.3535 mL | 2.7069 mL | 6.7674 mL | |
| 20 mM | 0.2030 mL | 1.0151 mL | 2.0302 mL | 5.0755 mL | |
| 25 mM | 0.1624 mL | 0.8121 mL | 1.6242 mL | 4.0604 mL | |
| 30 mM | 0.1353 mL | 0.6767 mL | 1.3535 mL | 3.3837 mL | |
| 40 mM | 0.1015 mL | 0.5076 mL | 1.0151 mL | 2.5378 mL | |
| 50 mM | 0.0812 mL | 0.4060 mL | 0.8121 mL | 2.0302 mL | |
| 60 mM | 0.0677 mL | 0.3384 mL | 0.6767 mL | 1.6918 mL | |
| 80 mM | 0.0508 mL | 0.2538 mL | 0.5076 mL | 1.2689 mL | |
| 100 mM | 0.0406 mL | 0.2030 mL | 0.4060 mL | 1.0151 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mafenide Acetate 関連分類
HY-B0614A 関連分類
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