Osajin
Based on 1 Customer Validation
Osajin is the major bioactive isoflavone present in the fruit of Maclura pomifera with antitumor, antioxidant and anti-inflammatory activities.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.18%
- CAS 番号: 482-53-1
- 分子式: C25H24O5
- 分子量:404.46
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Apoptosis[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| ACHN | GI50 |
12.13 μM
Compound: 1, osajin
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Growth inhibition of human ACHN cells after 21 hrs by MTT assay
Growth inhibition of human ACHN cells after 21 hrs by MTT assay
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[PMID: 17662606] |
| BMDM | IC50 |
12 μg/mL
Compound: 133
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Antiinflammatory activity in LPS-stimulated mouse BMDM cells assessed as inhibition of NF-kappaB signalling pathway incubated for 3 hrs by Western blot analysis
Antiinflammatory activity in LPS-stimulated mouse BMDM cells assessed as inhibition of NF-kappaB signalling pathway incubated for 3 hrs by Western blot analysis
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[PMID: 37683361] |
| HCT-15 | GI50 |
9.65 μM
Compound: 1, osajin
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Growth inhibition of human HCT15 cells after 21 hrs by MTT assay
Growth inhibition of human HCT15 cells after 21 hrs by MTT assay
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[PMID: 17662606] |
| HeLa | IC50 |
6.53 μM
Compound: 1, osajin
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Inhibition of HDAC in HeLa cells
Inhibition of HDAC in HeLa cells
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[PMID: 17662606] |
| Hepatocyte | GI50 |
>500 μM
Compound: 1, osajin
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Growth inhibition of human hepatocytes after 21 hrs by MTT assay
Growth inhibition of human hepatocytes after 21 hrs by MTT assay
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[PMID: 17662606] |
| LOX IMVI | GI50 |
10.98 μM
Compound: 1, osajin
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Growth inhibition of human LOX-IMVI cells after 21 hrs by MTT assay
Growth inhibition of human LOX-IMVI cells after 21 hrs by MTT assay
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[PMID: 17662606] |
| MDA-MB-231 | GI50 |
11.34 μM
Compound: 1, osajin
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Growth inhibition of human MDA-MB-231 cells after 21 hrs by MTT assay
Growth inhibition of human MDA-MB-231 cells after 21 hrs by MTT assay
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[PMID: 17662606] |
| NCI-H23 | GI50 |
11.97 μM
Compound: 1, osajin
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Growth inhibition of human NCI-H23 cells after 21 hrs by MTT assay
Growth inhibition of human NCI-H23 cells after 21 hrs by MTT assay
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[PMID: 17662606] |
| PC-3 | GI50 |
12.32 μM
Compound: 1, osajin
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Growth inhibition of human PC3 cells after 21 hrs by MTT assay
Growth inhibition of human PC3 cells after 21 hrs by MTT assay
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[PMID: 17662606] |
| RAW264.7 | IC50 |
7.2 μg/mL
Compound: 133
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Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced iNOS mRNA expression
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced iNOS mRNA expression
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[PMID: 37683361] |
| Vero | CC50 |
11.44 μM
Compound: Osajin
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Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr
Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr
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10.1101/2020.03.20.999730 |
| Vero | IC50 |
3.87 μM
Compound: Osajin
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Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr
Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr
|
10.1101/2020.03.20.999730 |
Osajin significantly decreases the viability of human NPC cells (TW076, CG1 and TW04 cells) in a dose-dependent manner. Osajin induces apoptosis in human NPC cells through multiple apoptotic pathways, including the extrinsic death receptor pathway, and intrinsic pathways relying on mitochondria and endoplasmic reticulum stress[1]. Osajin exhibits growth inhibitory activity on six human cancer cell lines, including kidney, lung, prostate, breast, melanoma and colon cancer cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 482-53-1
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性状 Solid
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分子量 404.46
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分子式 C25H24O5
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Color White to off-white
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SMILES
O=C1C2=C(O)C(C/C=C(C)\C)=C3C(C=CC(C)(C)O3)=C2OC=C1C4=CC=C(O)C=C4
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別名
CID 95168; NSC 21565
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
プロトコル
NPC cells are seeded in 96-well plates (5000/well) and allowed to adhere for 24 h. The medium is then substituted by a fresh one containing different concentrations (0.01–10 µM) of osajin for another 24 h. For the time-course assay, the incubation times with 5 µM of osajin are 6, 12, 24, 36 and 48 h. After incubation, 10 µl of 5 mg/ml of MTT is added to each well and incubated for 4 h at 37°C. Cell viability is determined using the MTT-based assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Rats[1]
The Wistar rats are divided into four groups. The first treatment group receives osajin (5 mg/kg/day in 0.5% Avicel); the second treatment group receives pomiferin (5 mg/kg/day in 0.5% Avicel); the placebo group receives only 0.5 Avicel; the last is an untreated control group. Biochemical indicator of oxidative damage-lipid peroxidation product malondialdehyde, antioxidant enzymes-superoxide dismutase, glutathione peroxidase, total antioxidant activity in serum and myocardium are evaluated. The effect of osajin and pomiferin on cardiac function, left ventricular end-diastolic pressure, left ventricular pressure and peak positive +dP/dt ischemia and reperfusion, also is examined[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
純度とドキュメンテーション
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データシート (276 KB)
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SDS (458 KB)
- English - EN (458 KB)
- Français - FR (458 KB)
- Deutsch - DE (458 KB)
- Norwegian - NO (458 KB)
- Español - ES (458 KB)
- Swedish - SV (458 KB)
- Italian - IT (458 KB)
- Korean - KR (458 KB)
- Portuguese - PT (458 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Huang TT, et al. Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin. PLoS One. 2011 Apr 12;6(4):e18308. [Content Brief]
[2]. Son IH, et al. Pomiferin, histone deacetylase inhibitor isolated from the fruits of Maclura pomifera. Bioorg Med Chem Lett. 2007 Sep 1;17(17):4753-5. [Content Brief]
[3]. Florian T, et al. Effects of prenylated isoflavones osajin and pomiferin in premedication on heart ischemia-reperfusion. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2006 Jul;150(1):93-100. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
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