DCG04
Based on 1 publication(s) in Google Scholar
DCG04 is a biotinylated activity-based probe. DCG04 labels calpain-1 and is applicable for affinity purification and mass spectrometry identification. DCG04 is used in cancer and malaria research.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.55%
- CAS 番号: 314263-42-8
- 分子式: C43H66N8O11S
- 分子量:903.10
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保管条件:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 DCG04
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生物活性
製品説明
体外実験
DCG04 reveals a progressive increase in cathepsin activity during RIP1-Tag2 tumorigenesis, identifying cathepsins B, C, L, and Z as active proteases in tumors[1].
DCG04 profiling of flow-sorted RIP1-Tag2 tumor cells shows that infiltrating innate immune cells are a significant source of cathepsin activity, with distinct activity profiles among endothelial, immune, and tumor cell fractions[1].
DCG-04 (50 μM) is an activity-dependent affinity label that covalently modifies multiple active cysteine proteases in DC2.4 dendritic cell extracts at pH 5.5[2].
DCG-04 affinity labeling enriches a 27-kDa cysteine protease from bovine chromaffin granules that is responsible for PE-processing activity[3].
DCG04 activity labeling confirms that immunodepletion and calpastatin loading abolish calpain-1 activity in resealed erythrocytes, which is restored by calpain-1 reconstitution[8].
DCG04 (4 μM; 4 h) is a component of the biotinylated probe cocktail used for ABPP-MS to display active cysteine proteases in N. benthamiana apoplastic fluid, revealing that bacterial infection reduces the activity of nine cysteine proteases[11].
DCG04 (~48 hours postinfection) labels a single ~80 kD membrane-associated protein in P. falciparum-infected erythrocytes, identified as human calpain-1[8].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 314263-42-8
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性状 Solid
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分子量 903.10
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分子式 C43H66N8O11S
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Color White to off-white
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SMILES
OC(C=C1)=CC=C1C[C@@H](C(NCCCCCC(N[C@H](C(N)=O)CCCCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O)=O)=O)NC([C@H](CC(C)C)NC([C@@H]4[C@H](O4)C(OCC)=O)=O)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
溶剤 & 溶解度
体外:
DMSO : 3.33 mg/mL (3.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (280 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1073 mL | 5.5365 mL | 11.0730 mL | 27.6824 mL |
DCG04 関連分類
HY-101779 関連分類
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