Hexadecyl-CoA
Based on 1 Customer Validation
Hexadecyl-CoA is a non-hydrolyzable thioether analog of Palmitoyl-CoA. Hexadecyl-CoA competitively inhibits the acyl-CoA thioesterase activity of HNF-4α with a Ki of 0.3 μM, and inhibits DGAT2 activity with an IC50 of approximately 2 μM. Hexadecyl-CoA also inhibits ATGL activity and serves as a reference inhibitor for DGAT2 LC/MS enzyme activity assays. Hexadecyl-CoA can be used in studies related to lipid metabolism, triglyceride synthesis and lipolysis.
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- CAS 番号: 71425-89-3
- 分子式: C37H68N7O16P3S
- 分子量:991.96
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
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HNF-4α1 0.3 μM (Ki) |
DGAT2 2 μM (IC50) |
体外実験
Hexadecyl-CoA (50 μM; 20 min) inhibits TG hydrolase activity of ATGL in COS-7 cell lysates co-expressing ATGL and CGI-58, reducing activity to ~20% of control at 50 μM[1].
Hexadecyl-CoA (1-2.0 μM) potently inhibits the acyl-CoA thioesterase activity of recombinant rat HNF-4α1-LBD (aa132-455) with an apparent Ki of 0.3 μM, via competitive binding that requires its CoA-thioether structure[2].
Hexadecyl-CoA (10 μM) binds specifically to the acyl-CoA site (ACS) of recombinant rat HNF-4α1-LBD (aa132-455) with a stoichiometry of 0.8 nmol per nmol protein, is excluded from the fatty acid pocket (FAP), and requires the intact E-F domain for high-affinity binding[2].
Hexadecyl-CoA (up to 100 μM) does not displace the FAP-bound fluorescent marker C12-Bodipy from human HNF-4α1 recombinant, demonstrating it is excluded from the fatty acid pocket[2].
Hexadecyl-CoA (0.001-100 μM; 60 min) potently inhibits DGAT2 activity in human DGAT2-overexpressing Sf9 insect cell membranes with an IC50 of approximately 2 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 71425-89-3
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性状 Solid
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分子量 991.96
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分子式 C37H68N7O16P3S
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Color White to off-white
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SMILES
O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(OP(O)(OCC(C)([C@H](C(NCCC(NCCSCCCCCCCCCCCCCCCC)=O)=O)O)C)=O)=O)O[C@H]1N2C3=NC=NC(N)=C3N=C2
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
溶剤 & 溶解度
体外:
H2O : ≥ 50 mg/mL (50.41 mM)
* "≥" means soluble, but saturation unknown.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.0081 mL | 5.0405 mL | 10.0811 mL | 25.2026 mL |
| 5 mM | 0.2016 mL | 1.0081 mL | 2.0162 mL | 5.0405 mL | |
| 10 mM | 0.1008 mL | 0.5041 mL | 1.0081 mL | 2.5203 mL | |
| 15 mM | 0.0672 mL | 0.3360 mL | 0.6721 mL | 1.6802 mL | |
| 20 mM | 0.0504 mL | 0.2520 mL | 0.5041 mL | 1.2601 mL | |
| 25 mM | 0.0403 mL | 0.2016 mL | 0.4032 mL | 1.0081 mL | |
| 30 mM | 0.0336 mL | 0.1680 mL | 0.3360 mL | 0.8401 mL | |
| 40 mM | 0.0252 mL | 0.1260 mL | 0.2520 mL | 0.6301 mL | |
| 50 mM | 0.0202 mL | 0.1008 mL | 0.2016 mL | 0.5041 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Hexadecyl-CoA 関連分類
HY-E70277 関連分類
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