IWP-3

IWP-3 potently induces cardiogenesis in day 4 mesodermal cells derived from human embryonic stem cells, with an EC₅₀ of 1214 nM. It drives mesodermal progenitor cells to form cardiomyocytes without expanding other mesodermal lineages^[1].
IWP-3 (10 μM) does not inhibit the kinase activity of bovine GST-CK1α, exerts moderate inhibitory effects on the kinase activities of human GST-CK1γ3 and human CK1ε, and significantly inhibits the purified kinase activities of rat GST-CK1δ and rat CK1δ^KD[2].
IWP-3 inhibits purified rat CK1δKD, GST-wtCK1δ and GST-M82FCK1δ with IC₅₀ values of 0.55 μM, 1.89 μM and 0.15 μM, respectively^[2].
IWP-3 (0.313 μM-10 μM; 48 h) inhibits the proliferation of MiaPaCa2, Panc-1, Panc-89, HEK293 and SW620 cancer cell lines, with EC₅₀ values ranging from 3.50 μM to 8.62 μM; in contrast, it exerts no inhibitory effect on the proliferation of A818-6, HT29 and Capan1 cells even at concentrations as high as 10 μM^[2].
IWP-3 (0.45-45 µM; 1-4 h) causes significant shortening of germband length at 2 h and 3 h of incubation and a significant reduction in cell proliferation at 2 h of incubation when applied to dissected Tribolium castaneum EFA-nGFP transgenic embryos^[3].
IWP-3 (45 µM; 2 h) significantly reduces total cell displacement and net cell movement across all germband regions, and decreases the proportion of cells moving along the anterior-posterior axis during 2-hour live-cell imaging when treated on dissected Tribolium castaneum EFA-nGFP transgenic embryos^[3].
IWP-3 is a Wnt production inhibitor with an IC₅₀ of 40 nM; it blocks palmitoylation of Wnt protein by inhibiting Porcn^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.