Propyl pyrazole triol
Based on 19 publication(s) in Google Scholar
Propyl pyrazole triol (PPT) is a selective estrogen receptor alpha (ERα) agonist. The relative binding affinity of Propyl pyrazole triol for ERα (ERα: 49%) around 410 times higher compared with estrogen receptor beta (ERβ: 0.12%).
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.11%
- CAS 番号: 263717-53-9
- 分子式: C24H22N2O3
- 分子量:386.44
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保管条件:Powder -20°C, 3 years ; In solvent -80°C, 2 years , -20°C, 1 year
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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MedChemExpress（MCE）の使用を引用している文献 Propyl pyrazole triol
More- Cancer Lett. 2021 Feb 1;498:54-69. [Abstract]
- Int J Biol Sci. 2022 Feb 21;18(5):1961-1973. [Abstract]
- Environ Health Perspect. 2024 Feb;132(2):27011. [Abstract]
- J Agric Food Chem. 2021 Dec 8;69(48):14544-14556. [Abstract]
- Ecotoxicol Environ Saf. 2023 Jul 1:259:115060. [Abstract]
- Eur J Pharmacol. 2022 Jun 5;924:174939. [Abstract]
- Int Immunopharmacol. 2024 Oct 18;143(Pt 2):113425. [Abstract]
- Mol Neurobiol. 2022 Nov;59(11):6934-6955. [Abstract]
- J Clin Endocrinol Metab. 2021 Apr 23;106(5):1410-1426. [Abstract]
- Molecules. 2020 Apr 23;25(8):1960. [Abstract]
- J Dairy Sci. 2026 Jan 20:S0022-0302(26)00013-5. [Abstract]
- Appl Biochem Biotechnol. 2022 Jun;194(6):2492-2502. [Abstract]
- PLoS One. 2026 May 14;21(5):e0349371. [Abstract]
- Biochem Biophys Res Commun. 2021 Nov 2:583:184-191. [Abstract]
- bioRxiv. 2025 Sep 12.
- Johns Hopkins University. 2025 Sep.
- Authorea. 2025 Mar 10.
- J Cancer Ther. 2022 Mar;13(3):117-130. [Abstract]
- Research Square Preprint. 2021 Jul.
Customer Validation & Images
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Flow Cytometry
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Flow Cytometry
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Bio/Physico-chemical Assay
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Cell Proliferation/Viability Assay
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Cell Migration/Invasion Assay
生物活性
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ERα |
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Cell Line
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Type | Value | Description | References |
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| MCF7 | IC50 |
27.2 μM
Compound: PPT
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Cytotoxicity in hormone-dependent human MCF7 cells assessed as growth inhibition incubated for 4 day s by crystal violet assay
Cytotoxicity in hormone-dependent human MCF7 cells assessed as growth inhibition incubated for 4 day s by crystal violet assay
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[PMID: 23043242] |
| U2OS | EC50 |
0.1 nM
Compound: PPT
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Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control
Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control
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[PMID: 23043242] |
The EC50 of Propyl pyrazole triol for the ERα-dependent response is 140 pM in ERα-U2OS-Luc cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 263717-53-9
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性状 Solid
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分子量 386.44
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分子式 C24H22N2O3
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Color White to off-white
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SMILES
CCCC1=C(C2=CC=C(O)C=C2)N(C3=CC=C(O)C=C3)N=C1C4=CC=C(O)C=C4
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別名
PPT
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years In solvent -80°C 2 years -20°C 1 year
Publications (19)
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Journal Impact Factor
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Most Recent
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Cancer Lett
The GPER1/SPOP axis mediates ubiquitination-dependent degradation of ERα to inhibit the growth of breast cancer induced by oestrogen. [Abstract]2021 Feb 1;498:54-69. PMID: 33069770
Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Feb 1;498:54-69. [Abstract]
ERα-specific agonist Propyl pyrazole triol (PPT) (1 nM; 48 h) enhanced luciferase activity in MCF-7 cells.
Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Feb 1;498:54-69. [Abstract]
ERα-specific agonist Propyl pyrazole triol (PPT) (1 nM; 48 h) induced proliferation of MCF-7 cells.
Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Feb 1;498:54-69. [Abstract]
ERα-specific agonist Propyl pyrazole triol (PPT) (1 nM; 48 h) induced migration and invasion of MCF-7 cells.
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Int J Biol Sci
SCM-198 Alleviates Endometriosis by Suppressing Estrogen-ERα mediated Differentiation and Function of CD4+CD25+ Regulatory T Cells. [Abstract]2022 Feb 21;18(5):1961-1973. PMID: 35342349
Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Feb 21;18(5):1961-1973. [Abstract]
Propyl pyrazole triol (PPT) (5 nM) promoted the expansion of CD4+CD25+ peritoneal regulatory T cells (Tregs).
Propyl pyrazole triol purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Feb 21;18(5):1961-1973. [Abstract]
Propyl pyrazole triol (PPT) (5 nM) notably increased the production of IL-10 and TGF-β1 in CD4+CD25+ peritoneal regulatory T cells (Tregs).
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Environ Health Perspect
Combined Effects of Polystyrene Nanosphere and Homosolate Exposures on Estrogenic End Points in MCF-7 Cells and Zebrafish. [Abstract]2024 Feb;132(2):27011. PMID: 38381479 -
J Agric Food Chem
Estrogen Receptor α Regulates Metabolic-Associated Fatty Liver Disease by Targeting NLRP3-GSDMD Axis-Mediated Hepatocyte Pyroptosis. [Abstract]2021 Dec 8;69(48):14544-14556. PMID: 34817168 -
Ecotoxicol Environ Saf
Bisphenol A interferes with lncRNA Fhadlos2 and RUNX3 association in adolescent mouse ovary. [Abstract]2023 Jul 1:259:115060. PMID: 37229876 -
Eur J Pharmacol
Estradiol reduced 5-HT reuptake by downregulating the gene expression of Plasma Membrane Monoamine Transporter (PMAT, Slc29a4) through estrogen receptor β and the MAPK/ERK signaling pathway. [Abstract]2022 Jun 5;924:174939. PMID: 35398393 -
Int Immunopharmacol
Estrogen receptor α aggravates intestinal inflammation via promoting the activation of NLRP3 inflammasome. [Abstract]2024 Oct 18;143(Pt 2):113425. PMID: 39426237 -
Mol Neurobiol
Isobavachalcone's Alleviation of Pyroptosis Contributes to Enhanced Apoptosis in Glioblastoma: Possible Involvement of NLRP3. [Abstract]2022 Nov;59(11):6934-6955. PMID: 36053436 -
J Clin Endocrinol Metab
2021 Apr 23;106(5):1410-1426. PMID: 33524147 -
Molecules
Apigenin Inhibits Histamine-Induced Cervical Cancer Tumor Growth by Regulating Estrogen Receptor Expression. [Abstract]2020 Apr 23;25(8):1960. PMID: 32340124 -
J Dairy Sci
ERα-NOS/NO/cGMP/PKG-mTOR/KITL/FOXO3a signaling cascade mediates estrogen-induced primordial follicle activation in goats. [Abstract]2026 Jan 20:S0022-0302(26)00013-5. PMID: 41570914 -
Appl Biochem Biotechnol
ERα, but not ERβ and GPER, Mediates Estradiol-Induced Secretion of TSH in Mouse Pituitary. [Abstract]2022 Jun;194(6):2492-2502. PMID: 35138554 -
PLoS One
2026 May 14;21(5):e0349371. PMID: 42133665 -
Biochem Biophys Res Commun
Ginsenoside (20S)-protopanaxatriol induces non-protective autophagy and apoptosis by inhibiting Akt/mTOR signaling pathway in triple-negative breast cancer cells. [Abstract]2021 Nov 2:583:184-191. PMID: 34749235 -
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J Cancer Ther
2022 Mar;13(3):117-130. PMID: 36311820 -
溶剤 & 溶解度
DMSO : ≥ 100 mg/mL (258.77 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (12.94 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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参考文献
[1]. Influence of cellular ERalpha/ERbeta ratio on the ERalpha-agonist induced proliferation of human T47D breast cancer cells. [Content Brief]
[2]. Stauffer SR, et al. Pyrazole ligands: structure-affinity/activity relationships and estrogen receptor-alpha-selective agonists. J Med Chem. 2000 Dec 28;43(26):4934-47. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5877 mL | 12.9386 mL | 25.8772 mL | 64.6931 mL |
| 5 mM | 0.5175 mL | 2.5877 mL | 5.1754 mL | 12.9386 mL | |
| 10 mM | 0.2588 mL | 1.2939 mL | 2.5877 mL | 6.4693 mL | |
| 15 mM | 0.1725 mL | 0.8626 mL | 1.7251 mL | 4.3129 mL | |
| 20 mM | 0.1294 mL | 0.6469 mL | 1.2939 mL | 3.2347 mL | |
| 25 mM | 0.1035 mL | 0.5175 mL | 1.0351 mL | 2.5877 mL | |
| 30 mM | 0.0863 mL | 0.4313 mL | 0.8626 mL | 2.1564 mL | |
| 40 mM | 0.0647 mL | 0.3235 mL | 0.6469 mL | 1.6173 mL | |
| 50 mM | 0.0518 mL | 0.2588 mL | 0.5175 mL | 1.2939 mL | |
| 60 mM | 0.0431 mL | 0.2156 mL | 0.4313 mL | 1.0782 mL | |
| 80 mM | 0.0323 mL | 0.1617 mL | 0.3235 mL | 0.8087 mL | |
| 100 mM | 0.0259 mL | 0.1294 mL | 0.2588 mL | 0.6469 mL |
Propyl pyrazole triol 関連分類
HY-100689 関連分類
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