TOTO-3
Based on 1 Customer Validation
TOTO-3 is a cyanine nucleic acid intercalating fluorescent probe that binds to both RNA and DNA (Ex=642 nm, Em=660 nm). TOTO-3 cannot enter intact cells, but can penetrate cells with permeabilized plasma membranes, staining cytoplasmic RNA and nucleoli to distinguish intact cells from damaged ones. TOTO-3 is taken up by macrophages in an ultrasound-independent manner. TOTO-3 accumulates in tumor cells via ultrasound-mediated enhancement of plasma membrane permeability. TOTO-3 supports optical imaging for real-time assessment of plasma membrane integrity, nucleic acid detection, and monitoring of ultrasound-mediated intracellular delivery processes. TOTO-3 is applicable to studies related to rectal cancer.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 85.0%
- CAS 番号: 166196-17-4
- 分子式: C53H62I4N6S2
- 分子量:1354.85
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保管条件:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
TOTO-3 is a cell-impermeable dye, as it only enters and stains mouse rectal carcinoma line CMT-93 cells[1].
TOTO-3 (1 μM; 5 min at 37°C) discriminates between intact and membrane-compromised human HaCaT keratinocyte cells and human M4Beu melanoma cells, enabling selective analysis of viable cells for Ursolic acid (HY-N0140)-induced mitochondrial transmembrane potential changes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 166196-17-4
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性状 Solid
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分子量 1354.85
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分子式 C53H62I4N6S2
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Color Brown to black
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SMILES
CN1C2=CC=CC=C2S/C1=C\C=C\C3=CC=[N+](CCC[N+](C)(CCC[N+](C)(CCC[N+]4=CC=C(/C=C/C=C5SC6=C(C=CC=C6)N/5C)C7=C4C=CC=C7)C)C)C8=C3C=CC=C8.[I-].[I-].[I-].[I-]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
DMSO : 50 mg/mL (36.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7381 mL | 3.6904 mL | 7.3809 mL | 18.4522 mL |
| 5 mM | 0.1476 mL | 0.7381 mL | 1.4762 mL | 3.6904 mL | |
| 10 mM | 0.0738 mL | 0.3690 mL | 0.7381 mL | 1.8452 mL | |
| 15 mM | 0.0492 mL | 0.2460 mL | 0.4921 mL | 1.2301 mL | |
| 20 mM | 0.0369 mL | 0.1845 mL | 0.3690 mL | 0.9226 mL | |
| 25 mM | 0.0295 mL | 0.1476 mL | 0.2952 mL | 0.7381 mL | |
| 30 mM | 0.0246 mL | 0.1230 mL | 0.2460 mL | 0.6151 mL |
TOTO-3 関連分類
HY-D0920 関連分類
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